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3Rs in Ecotoxicity Testing

To regulate new chemical products and pollution from industry and agriculture, government agencies mandate toxicity tests to determine how substances effect the environment. Organizations are prioritizing the development and validation of non-animal testing methods called new approach methodologies (NAMs) to conduct these toxicity tests. NAMs refers to any technology, methodology, or approach that provides information on chemical hazard, exposure, or risk assessment (e.g., "organ-on-a-chip", computer models, in chemico assays). On this page find links to research articles and testing tools provided by federal agencies. 

Alternatives in Ecotoxicology Literature

New Approach Methodologies (NAMs) in Ecotoxicology

microfluidic device

This bibliography provides citations regarding new approach methodologies (NAMs) used in the field of Ecotoxicology. NAMs are non-animal technologies and approaches used for chemical hazard, exposure and risk assessment testing such as in vitro assays, computational models, cell-based models, and organs-on-chips. The bibliography covers peer-reviewed literature published between 2010 and July 2024.

Ecotoxicity Testing Tools

Computational Toxicology Projects

DHHSNIEHS. National Toxicology Program Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM). 

Tools and data used to predict toxicity, such as the Integrated Chemical Environment (ICE). 

ECOTOX Knowledgebase

U.S. Environmental Protection Agency. 

A comprehensive system providing chemical environmental toxicity data on aquatic and terrestrial species. 

SeqAPASS: Sequence alignment to predict across-species susceptibility

U.S. Environmental Protection Agency.

An online database that allows researchers and regulators to extrapolate toxicity information across species. Requires a (free) account to access.

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