Research Publications (Food Safety)

Toxins, Vol. 11, Pages 502: Genetics, Toxicity, and Distribution of Enterohemorrhagic Escherichia coli Hemolysin

Toxins doi: 10.3390/toxins11090502

Authors:
Maike Schwidder
Laura Heinisch
Herbert Schmidt

Toxins, Vol. 11, Pages 505: Baicalein Inhibits Stx1 and 2 of EHE: Effects of Baicalein on the Cytotoxicity, Production, and Secretion of Shiga Toxins of Enterohaemorrhagic Escherichia coli

Toxins doi: 10.3390/toxins11090505

Authors:
Vinh
Shinohara
Yamada
Duc
Nakayama
Ozawa
Sato
Masuda
Honjoh
Miyamoto

Sandhya Ganesan, Craig R. Roy

Abstract

Aims

Given the significance of Salmonella enterica to both human and animal health, and a recent global dissemination of Salmonella 4,[5],12:i:‐, changes in the prevalent serovars and antimicrobial resistance in clinical isolates of Salmonella from cattle and pigs were investigated in Japan.

Abstract

Abstract

Available online 26 August 2019

Author(s): Valeria A. Torok, Kate R. Hodgson, Jessica Jolley, Alison Turnbull, Catherine McLeod

Available online 26 August 2019

Author(s): Denes K.A. Rosario, Yago A.A. Bernardo, Yhan S. Mutz, Brijesh Tiwari, Andreja Rajkovic, Patricia C. Bernardes, Carlos A. Conte-Junior

Toxins, Vol. 11, Pages 500: Distribution of Toxinogenic Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus from Different Ecological Niches in Algeria

Toxins doi: 10.3390/toxins11090500

Authors:
Assia Mairi
Abdelaziz Touati
Alix Pantel
Karima Zenati
Alex Yahiaoui Martinez
Catherine Dunyach-Remy
Albert Sotto
Jean-Philippe Lavigne

A comprehensive multiresidue pesticide analysis for Cannabis flower matrices using quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation, liquid chromatography paired with triple quadrupole mass spectrometry (LC–MS/MS), and gas chromatography paired with triple quadrupole mass spectrometry (GC–MS/MS) was established and validated for 367 pesticides with excellent recoveries (70–120%) for over 99% of analytes included.

Cronobacter strains harboring CRISPR-Cas systems are important foodborne pathogens that cause serious neonatal infections. CRISPR typing is a new molecular subtyping method to track the sources of pathogenic bacterial outbreaks and shows a promise in typing Cronobacter, however, this molecular typing procedure using routine PCR method has not been established. Therefore, the purpose of this study was to establish such methodology, 257 isolates of Cronobacter sakazakii, C. malonaticus, and C.

Abstract

Abstract

Abstract

Toxins, Vol. 11, Pages 493: A Novel Magnetic Molecular Imprinted Polymer for Selective Extraction of Zearalenone from Cereal Flours before Liquid Chromatography-Tandem Mass Spectrometry Determination

Toxins doi: 10.3390/toxins11090493

Authors:
Chiara Cavaliere
Michela Antonelli
Andrea Cerrato
Giorgia La Barbera
Aldo Laganà
Michele Laus
Susy Piovesana
Anna Laura Capriotti

Toxins, Vol. 11, Pages 495: Influence of Two Garlic-Derived Compounds, Propyl Propane Thiosulfonate (PTS) and Propyl Propane Thiosulfinate (PTSO), on Growth and Mycotoxin Production by Fusarium Species In Vitro and in Stored Cereals

Toxins doi: 10.3390/toxins11090495

Authors:
Kalliopi Mylona
Esther Garcia-Cela
Michael Sulyok
Angel Medina
Naresh Magan

Toxins, Vol. 11, Pages 499: Acrylamide Reduction Strategy in Combination with Deoxynivalenol Mitigation in Industrial Biscuits Production

Toxins doi: 10.3390/toxins11090499

Authors:
Suman
Generotti
Cirlini
Dall’Asta

Natural microcin J25 (MccJ25) represent promising alternatives to traditional antibiotics for the treatment of drug-resistant infections. However, little is known about the antibacterial activity of recombinant MccJ25 against foodborne pathogens. Here, the activity of recombinant MccJ25 was examined using a matrix of conditions in order to assess the efficacy of recombinant MccJ25 as a mitigation against foodborne pathogens, Salmonella species and Escherichia coli (E. coli) O157.

This study aimed to determine the antimicrobial activity of thymoquinone (TQ) against L. monocytogenes, and to examine its inhibitory effects on biofilm formation, motility, hemolysin production, and attachment-invasion of host cells. The minimum inhibitory concentrations (MICs) of TQ against eight different L. monocytogenes strains ranged from 6.25–12.50 μg/mL. Crystal violet staining showed that TQ clearly reduced biofilm biomass at sub-MICs in a dose-dependent manner.

Cyclospora cayetanensis is a coccidian parasite that has been associated with foodborne outbreaks of gastrointestinal illnesses. Raspberries have been implicated as vehicle of infection in some of these outbreaks. Most of the molecular techniques used for the detection of parasites commonly use the 18s rRNA as a target gene, which is highly conserved.

Coxiella burnetii is an obligate intracellular gammaproteobacterium and zoonotic agent of Q fever. We previously identified 15 small non-coding RNAs (sRNAs) of C. burnetii. One of them, CbsR12 (Coxiella b urnetii small RNA 12), is highly transcribed during axenic growth and becomes more prominent during infection of cultured mammalian cells. Secondary structure predictions of CbsR12 revealed four putative CsrA-binding sites in stem loops with consensus AGGA/ANGGA motifs.

Laboratory surveillance plays an important role in the detection and control of hepatitis A outbreaks and requires the application of rapid and accurate molecular diagnostic tools for hepatitis A virus (HAV) RNA detection, subgenotype identification, and sequence-based genotyping.

The conventional methodology for gastrointestinal pathogen detection remains time-consuming, expensive, and of limited sensitivity. The objective of this study was to evaluate the performance of the BD Max enteric viral panel (Max EVP) assay for identification of viral pathogens in stool specimens from individuals with symptoms of acute gastroenteritis, enteritis, or colitis.

Publication date: Available online 6 August 2019

Environmental Research

Author(s): Alix F. Leblanc, Eléonore A. Attignon, Emilie Distel, Spyros P. Karakitsios, Dimosthenis A. Sarigiannis, Sylvie Bortoli, Robert Barouki, Xavier Coumoul, Martine Aggerbeck, Etienne B. Blanc

Publication date: Available online 10 August 2019

Environmental Research

Author(s): Ron Shadbegian, Dennis Guignet, Heather Klemick, Linda Bui

Abstract

Background

There is consensus that early childhood lead exposure causes adverse cognitive and behavioral effects, even at blood lead levels (BLL) below 5 μg/dL. What has not been established is to what extent the effects of childhood lead exposure persist across grades.