Research Publications (Food Safety)

The purpose of this study is to apply the visual qualitative (LFD-LAMP) and advanced quantitative loop-mediated isothermal amplification (EVA-LAMP) methods to detect Listeria monocytogenes, which is an important pathogenic foodborne bacterium. This research designed a new set of primers to amplify the hlyA gene of L. monocytogenes. The LFD-LAMP products were detected by lateral flow device (LFD) with the naked eyes, and the test results were consistent with agarose gel electrophoresis.

Ingredients and complexity of food matrices are inhibitory factors for gene expression studies of bacterial pathogens directly from food matrix. The aim of this study was to develop a simple and fast method for RNA extraction from ground beef matrix as well as stx genes expression of Escherichia coli O157:H7 by relative quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR).

Publication date: March 2016
, Volume 61
Author(s): Yanjun Shen, Jingliang Kang, Weitao Chen, Shunping He

Publication date: March 2016
, Volume 61
Author(s): Sharmili Roy, Ibrahim Abd Rahman, Jose Hernandez Santos, Minhaz Uddin Ahmed

Publication date: March 2016
, Volume 61
Author(s): Asmaa Galal-Khallaf, Alba Ardura, Yaisel J. Borrell, Eva Garcia-Vazquez

As a supplement to or extension of methods used to determine trends in foodborne illness over time, we propose the use of Internet search metrics. We compared Internet query data for foodborne illness syndrome–related search terms from the most popular 5 Korean search engines using Health Insurance Review and Assessment Service inpatient stay data for 26 International Classification of Diseases, Tenth Revision, codes for foodborne illness in South Korea during 2010–2012.

Publication date: February 2016
, Volume 53, Part B
Author(s): J. Stoops, S. Crauwels, M. Waud, J. Claes, B. Lievens, L. Van Campenhout

Publication date: February 2016
, Volume 53, Part B
Author(s): Anais Auvolat, Nathalie Gnanou Besse

Ricarda Maria Schmithausen, Sophia Veronika Schulze-Geisthoevel, Franziska Stemmer, Mohamed El-Jade, Marion Reif, Sylvia Hack, Alina Meilaender, Gabriele Montabauer, Rolf Fimmers, Marijo Parcina, Achim Hoerauf, Martin Exner, Brigitte Petersen, Gabriele Bierbaum, Isabelle Bekeredjian-Ding

Publication date: Available online 30 September 2015

Author(s): B. Wade, A.L. Keyburn, T. Seemann, J.I. Rood, R.J. Moore

C. George et al.

MICROBIOLOGY Correction for “The opportunistic marine pathogen Vibrio parahaemolyticus becomes virulent by acquiring a plasmid that expresses a deadly toxin,” by Chung-Te Lee, I-Tung Chen, Yi-Ting Yang, Tzu-Ping Ko, Yun-Tzu Huang, Jiun-Yan Huang, Ming-Fen Huang, Shin-Jen Lin, Chien-Yu Chen, Shih-Shuen Lin, Donald V. Lightner, Han-Ching Wang, Andrew H.-J. Wang, Hao-Ching...

Methylmercury (MeHg) is a potent neurotoxin that is biomagnified approximately 1–10 million-fold in aquatic carnivores such as the Northern elephant seal (Mirounga angustirostris), whose excreta and molted pelage, in turn, constitute a source of environmental MeHg contamination at the base of marine food chains. The potential for this top-down contamination...

Translation of polyproline proteins leads to translation arrest. To overcome this ribosome stalling effect, bacteria depend on a specialized translation elongation factor P (EF-P), being orthologous and functional identical to eukaryotic/archaeal elongation factor e/aIF-5A (recently renamed “EF5”). EF-P binds to the stalled ribosome between the peptidyl-tRNA binding and tRNA-exiting sites, and stimulates peptidyl-transferase activity thus allowing translation to resume.

High accumulations of phytoplankton species that produce toxins are referred to as harmful algal blooms (HABs). HABs represent one of the most important sources of contamination in marine environments, as well as a serious threat to public health, fisheries, aquaculture-based industries, and tourism. Therefore, methods effectively controlling HABs with minimal impact on marine ecology are required.

e-First Articles.

Publication date: January 2016
, Volume 111
Author(s): Annachiara Berardinelli, Frederique Pasquali, Chiara Cevoli, Marcello Trevisani, Luigi Ragni, Rocco Mancusi, Gerardo Manfreda

The effect of Ziziphora clinopodioides (0.1 and 0.2%) and nisin (250 and 500 IU/g), both separately and in combination, on mesophilic, psychrotrophic and Enterobacteriaceae microorganisms and also on Staphylococcus aureus and Escherichia coli O157:H7 in raw beef patty during storage at refrigerated temperature for 9 days were evaluated. Carvacrol (64.22%), followed by thymol (19.22%), p-cymene (4.86%) and γ-terpinene (4.63%) were the most abundant components of the essential oil.

This study aimed to investigate the effect of different temperatures on survival of various strains of Yersinia enterocolitica during the storage of ultra-filtered (UF) white cheese. UF pasteurized cow milk was inoculated with 3 log colony-forming unit per gram of two standard strains (DSM 11502 and DSM 9499) and one native strain of Y. enterocolitica. Cheese samples were prepared according to the regular procedure and stored at 14, 8 and 25C.

Vibrio parahaemolyticus is an important seafood-borne pathogen. V. parahaemolyticus carrying either tdh or trh or both genes is considered a pathogenic strain. The objectives of this study were to examine the prevalence and concentration of V. parahaemolyticus in shrimp processed in a factory using most probable number (MPN) combined with either polymerase chain reaction (PCR) or loop-mediated isothermal amplification (LAMP) techniques.

This study investigated the effect of material typespolystyrene, polypropylene, glass, and stainless steel) and glucose addition on Staphylococcus aureus biofilm formation, and the relationship between biofilm formation measured by crystal violet (CV) staining and the number of biofilm cells determined by cell counts was studied. We also evaluated the efficacy of chlorine sanitizer on inhibiting various different types of S. aureus biofilms on the surface of stainless steel.

Publication date: February 2016
, Volume 53, Part B
Author(s): Dominic Dussault, Khanh Dang Vu, Monique Lacroix