Research Publications (Food Safety)

In this study, a type of magnetic photoaffinity-labeled activity-based protein profiling probe for sulfonamide drugs was first synthesized for the purpose of capturing the natural dihydropteroate synthase of Escherichia coli by using simple incubation and magnetic separation. After characterization of its identity with LC-ESI-MS/MS, this enzyme was used as a recognition reagent to develop a direct competitive pseudo-ELISA for the determination of the residues of 40 sulfonamides in pork.

Trimethylamine N-oxide (TMAO) is a pro-atherosclerotic product of dietary choline metabolism generated by a microbiome–host axis. The first step in this pathway is the enzymatic metabolism of choline to trimethylamine (TMA) by the gut microbiota. This reaction could be targeted to reduce atherosclerosis risk.

Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) in poultry is most often transmitted by the fecal–oral route, which can be attributed to high population density. Upon encountering the innate immune response in a host, the pathogen triggers a stress response and virulence factors to help it survive in the host.

by Chayanee Noosak, Pavarish Jantorn, Jirut Meesane, Supayang Voravuthikunchai, Dennapa Saeloh Sericin, a natural protein from silk cocoon, has been reported for various biological properties in the biomaterials field. Modified forms of sericin have been studied for bone tissue engineering, while its unmodified form has been scarcely reported. Therefore, the purpose of this study was to evaluate physical and biological properties of unmodified sericin for potential use in bone surgery.

Milk, the most consumed dairy product, is often contaminated by the carcinogenic aflatoxin M1. Here, we present a one-step automatic sample pretreatment method using immunomagnetic beads and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for the detection of aflatoxin M1 in milk with good linearity and sensitivity (detection limit = 0.0008 μg/kg and quantitation limit = 0.0027 μg/kg).

We investigated the occurrence of aflatoxin M1 (AFM1) in yogurt and milk, including pasteurized, ultra-high temperature (UHT), and extended shelf life (ESL) milk samples, from central-eastern China and assessed the risk of AFM1 exposure in consumers.

Listeria (L.) monocytogenes is a significant pathogen found in ready-to-eat meat and dairy products. Soft cheeses, such as Queso Fresco cheese (QFC), are particularly sensitive to listeria contamination, and occasionally serve as a source of food-borne illness outbreaks. In the present study, clinical and cheese isolates of L.

Zearalenone (ZEN) and fumonisins (FUM) jeopardize fertility and health in cattle; yet, their toxigenic effects on rumen health and microbiota, both being crucial for animal health, are not clarified. This study determined the effects of a short-term exposure to ZEN or FUM on the rumen ecosystem, and further evaluated acute implications on health parameters.

The coagulase-negative staphylococcal (CoNS) species Staphylococcus lugdunensis is unique in causing serious infections in humans that resemble those of Staphylococcus aureus rather than those of other CoNS species. The colonization and invasion of host tissue presupposes the presence of adherence factors, but only a few proteins mediating adhesion of S. lugdunensis to biotic surfaces are known yet. Here, we report on the functionality of the S.

The worldwide emergence and spread of infections caused by multidrug-resistant bacteria endangers the efficacy of current antibiotics in the clinical setting. The lack of new antibiotics in the pipeline points to the need of developing new strategies. Recently, gold-based drugs are being repurposed for antibacterial applications. Among them, gold(III) complexes have received increasing attention as metal-based anticancer agents.

Campylobacter jejuni is a leading cause of food-borne disease worldwide. The pathogenicity of C. jejuni is closely associated with the internalization process in host epithelial cells, which is related to a host immune response. Autophagy indicates a key role in the innate immune system of the host to exclude invasive pathogens. Most bacteria are captured by autophagosomes and degraded by autophagosome-lysosome fusion in host cells.

Process impairing foam formation occurs regularly in batch distillation devices of the spirit industry. It negatively influences process and product quality. Up to now, such foam-related problems have not been in the focus of scientific investigations. This study aimed at preventing impairing foam formations by adapting the thermal energy input in fruit and grain mash distillations in larger scale batch distillations.

Seawater could be considered a reservoir of antibiotic-resistant bacteria and antibiotic resistance genes. In this communication, we evaluated the presence of bacterial strains in seawater collected from different coasts of Sicily by combining microbiological and molecular methods. Specifically, we isolated viable bacteria that were tested for their antibiotic resistance profile and detected both antibiotic and heavy metal resistance genes.

Salmonella enterica serovar Paratyphi C is highly adapted to humans and can cause a typhoid-like disease with high mortality rates. In this study, three serovar-specific genes were identified by comparative genomics for Salmonella Paratyphi C, SPC_0871, SPC_0872, and SPC_0908.

Journal of Food Processing and Preservation, Accepted Article. There are many problems of peanuts, such as lipid instability, high microbial content and aflatoxin. In this study, effect of superheated steam (SS) treatment on the security quality of grounded peanut (GP) was evaluated. Results indicated that concentrations of total bacteria, mold and Bacillus spp. were maximum reduced 99.26%, 100% and 98.57%, respectively.

Letters in Applied Microbiology, Accepted Article. In this work, the antibacterial activity and mechanism of chloroform fraction obtained from aqueous extract of mugwort leaves against Staphylococcus aureus (S. aureus) were investigated. The extract showed obvious antibacterial activity against S.

Aflatoxin-contaminated grain consumption over the years has been known to cause serious health hazards on its consumers. This study investigated the effects of harvest seasons and drying methods on aflatoxins in freshly harvested maize.

Cryptosporidium spp. and Giardia duodenalis are opportunistic zoonotic protozoa transmitted through several routes. In this study, a total of 604 fecal samples were collected from pet dogs in Xinjiang, China to detect the presence of Cryptosporidium spp. and G. duodenalis by PCR amplification of the small subunit (SSU) rRNA gene. The incidence rate of Cryptosporidium spp. or G. duodenalis was 5.3% (32/604).

Toxoplasma gondii infects approximately 1-2 billion people, and manipulation of the macrophage response is critical to host and parasite survival. A cleaved (cl)-CD95L form can promote cellular migration and we have previously shown that cl-CD95L aggravates inflammation and pathology in systemic lupus erythematosus (SLE). Findings have shown that CD95L is upregulated during human infection, therefore we examined the effect of cl-CD95L on the macrophage response to T.

Zoning is typically adopted by food manufacturers and classified into three different zones including Zone 1 (food contact surface), Zone 2 (proximity to food and food contact surfaces) and Zone 3 (remote surfaces from processing). In this study, environmental surfaces belonging to these three Zones were sampled during food processing in a FVs processing facility located in Beijing, China.

Twenty types of fruits and vegetables representing a broad range of fresh produce categories were assessed for the growth potential and growth kinetics of Listeria monocytogenes under conditions reflecting commercial practices for storage and distribution and conditions reflecting sustained temperature abuse. Whole and fresh-cut produce was obtained from various commercial sources and inoculated with L.

In order to assess the health risks associated with the consumption of Sardina pilchardus (Walbaum, 1792) and to study the spatiotemporal dynamics of three potentially toxic metals, lead (Pb), cadmium (Cd) and mercury (Hg) concentrations were measured in the muscle and the liver of 872 specimens of sardine sampled between October 2017 and September 2018 along the Algerian coast at 3 sites (Algiers, Dellys and Bejaia) subject to high pollution pressure.

Toxoplasma gondii (T. gondii) is a facultative heterogeneous parasite that belongs to Apicomplexa and can infect almost all warm-blooded animals, including ruminants, birds and humans. To date, no information is available about the molecular investigation of T. gondii in large ruminants from Pakistan.

UPLC-MS/MS and GC-MS/MS were used to establish a method to simultaneously determine various pesticide residues in Panax notoginseng. Results showed that the limits of detection of 249 pesticides were all 5–10 μg/kg. The detection rate of pesticides in 121 P. notoginseng samples was 93.39%, and 19 pesticides were detected.

Docosahexaenoic acid (DHA, 22:6n-3) plays significant roles in enhancing human health and preventing human diseases. The heterotrophic marine dinoflagellate Crypthecodinium cohnii is a good candidate to produce high-quality DHA. To overcome the inhibition caused by the fermentation supernatant in the late fermentation stage of DHA-producing C. cohnii, fermentation supernatant-based adaptive laboratory evolution (FS-ALE) was conducted.