Research Publications (Food Safety)

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1422-1434, February 2022. Aims Staphylococcus aureus is an opportunistic pathogen of humans. No commercial vaccine is available to combat S. aureus infections. In this study, we have investigated the protective immune response generated by S.

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1503-1517, February 2022. This study evaluated detection methods for Salmonella Typhi (S.

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1518-1525, February 2022. Aim The study aimed to investigate the role of cattle slaughterhouse wastewater as a possible source for the environmental distribution of Listeria monocytogenes. Methods and Results Listeria spp. isolation was performed by collecting 117 wastewater samples from four different cattle slaughterhouses in Turkey.

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1467-1478, February 2022. Aims The biological events occurring during human digestion help to understand the mechanisms underlying the dose–response relationships of enteric bacterial pathogens.

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1449-1456, February 2022. Aims We investigated the fate of Salmonella in lettuce seeds grown in a hydroponic system and the potentials of applying photodynamic inactivation (PDI) to enhance microbial safety of hydroponic farming systems. Methods and Results Lettuce was grown from Salmonella-contaminated seeds, and rose bengal-mediated PDI was applied.

Journal of Applied Microbiology, Volume 132, Issue 2, Page 760-771, February 2022. Aims To investigate the relationships between individual health status of oysters, particularly with regard to parasitic infection, and variability in abundance of human-pathogenic Vibrio species. Methods and Results Aquacultured eastern oysters, Crassostrea virginica, were analysed individually for infection by the protozoan parasite Perkinsus marin

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1457-1466, February 2022. Aims To generate a murine experimental model of colonization by Campylobacter coli DSPV458. Methods and Results Twelve adult Balb/cCmedc female mice were housed in a treated group (T-G) and a control group (C-G) for 4 weeks. Both experimental groups received antibiotics for 5 days during the first week.

Leafy green vegetables are a common source of Shiga toxin-producing Escherichia coli O157:H7 (STEC O157) foodborne illness outbreaks. Ruminant animals, primarily cattle, are the major reservoir of STEC O157. Epidemiological, traceback and field investigations were conducted to identify potential outbreak sources. Product and environmental samples were tested for STEC.

Our previous survey revealed a poor microbial quality of, and the presence of Salmonella on leafy green vegetables grown/sold in Accra, Ghana.  This study validated the efficacy of some cleaning/sanitation methods commonly used by Ghanaian households (tap water, salt solution, lemon juice, and vinegar), by comparing them with the performance of several sanitation approaches used by the U.S.

Sugar beet is major crop for sugar industry. With growing awareness on unsystematic use of pesticides, health problem and environmental issues, pesticide residues in soil and crops had became necessary for their assessment. Studies under subtropical conditions on dissipation/residue analysis of clopyralid are not reported. Therefore, dissipation kinetics and terminal residues of clopyralid for two cropping seasons in soil and sugar beet crop were studied under field conditions.

Toxoplasma gondii infects warm-blooded animals, including humans, and the ingestion of undercooked meat of infected animals is an important source of infection. We investigated the presence of T. gondii by PCR and histopathology in tissues of 25 seropositive sheep slaughtered for human consumption in Rio de Janeiro and assessed the suitability of different tissues for molecular detection of the parasite. The animals were first screened for T.

In recent years, the shrimp farming industry encountered significant economic losses induced by Vibrio alginolyticus. In this study, the influence of Vibrio alginolyticus on intestinal histomorphology and microbiome composition in Litopenaeus vannamei were studied.

Over 1 billion people rely on seafood as their primary source of protein and Vibrio spp. is the most important pathogen of foodborne disease outbreak related to consumption of seafood. Moreover, the rise of drug-resistant Vibrio strains further increases the difficulty of clinical treatment.

Emergence of antibiotic resistant bacteria is evolving at an alarming pace; therefore, we must start turning to alternative approaches. One of these, could be the use of antibiotic adjuvants that enhances the effect of antibiotics towards resistant bacteria. A novel antibiotic adjuvant is cannabidiol (CBD), which we have previously shown can enhance the effect of bacitracin (BAC).

To estimate the mean dietary exposure to total As (tAs) and some arsenic species [inorganic arsenic (iAs), methylarsonic acid (MMAs) and dimethylarsinic acid (DMAs), and arsenobetaine (AsB)] across the entire Japanese population (≥1 year old), a national total diet study (TDS) based on composite sample with a market-basket approach was conducted in Japan from 10 regions throughout Japan in 2019.

Quantitative, rapid, selective and sensitive methods for the determination of total arsenic (tAs) and six arsenic compounds (arsenite (As(III)), arsenate (As(V)), arsenobetaine (AsB), arsenocholine (AsC), monomethylarsonic acid (MMA), dimethylarsonic acid (DMA)) in seafood were developed. The measurement of the tAs concentration was performed using quadrupole inductively-coupled plasma mass spectrometry (ICP-MS). Microwave-assisted extraction was used for the isolation of arsenic species.

Aflatoxins are produced as secondary metabolites by the toxigenic Aspergillus fungi. Among the aflatoxins, aflatoxin B1 (AFB1) is a common contaminant of global concern in human and animal food products. Prolonged exposure to AFB1 may provoke hepatocyte pyroptosis and oxidative stress, which leads to liver damage. Dietary polyphenols could protect the liver from a wide range of toxins.

Bee pollen contains a diversity of bioactive components. Nevertheless, since pollen is retrieved from a variety of plants, including the cultivated ones which are subjected to agrochemical treatments, its contamination is unavoidable. In this context, 45 samples of pollen were analysed with optimized analytical methods for trace and macro elements (ICP-MS), pesticides and metabolites residues (LC & GC-MS/MS) content.

Procambarus clarkii (crayfish) accumulates a high concentration of Arsenic (As) from the aquatic environment and causes considerable human health risks. In this study, Limosilactobacillus fermentum GR-3 strain was isolated from “Jiangshui” and applied for As(III) adsorption and antioxidant abilities.

Long-term arsenic exposure is associated with diabetes in adults, the mechanism of which involves insulin resistance. The relationship between arsenic and insulin resistance in adults is unclear. We analyzed the relationship between urinary arsenic and insulin resistance in US adults.

This study aimed to investigate the subclinical symptom and histological lesions of 21-day-old and 42-day-old broilers exposure to low concentration aflatoxin B1 (AFB1), and the preventive effect with adsorbent (Toxo-MX) supplementation. A total of 576 one-day-old Arbor Acres broilers were randomly allotted into 6 treatments 8 replicates and 12 birds per cage, fed with 0 ppb, 60 ppb and 120 ppb AFB1 contamination diet with or without Toxo-MX supplementation.

Foods contaminated by foodborne pathogens have always been a great threat to human life. Herein, we constructed an electrochemical immunosensor for Salmonella detection by using a Fe3O4@graphene modified electrode.

Escherichia coli O157:H7, the causative agent of thrombotic thrombocytopenic purpura and hemolytic uremic syndrome in humans, generates a effective harm to community health because of its high pathogenicity. A real-time recombinase-aided amplification (rRAA) is an emerging method for nucleic acid detection.

Dairy cows can be reservoirs of foodborne pathogens such as Salmonella that pose serious public health risks to humans.

Naturally fermented filed water-dropwort (Oenanthe javanica) extracts by steeping with oligosaccharides (FDE) show a hepatoprotective activity against the liver injury induced by carbon tetrachloride (CCl4) and ethanol. However, the role of FDE in non-alcoholic steatohepatitis (NASH) has not yet been reported. In the present study, we investigated the effect of FDE on NASH using a mouse model with a methionine/choline-deficient diet (MCD).