Research Publications (Food Safety)

The role of iron plaque in Miscanthus sacchariflorus seedling growth and cadmium ion (Cd2+) uptake, translocation, and tolerance was explored. The seedlings were cultivated for 10 d in half-strength Hoagland’s nutrient solution containing 0 mg L−1, 1.0 mg L−1, 5.0 mg L−1, 10.0 mg L−1, and 20.0 mg L−1 Cd2+ after iron plaque formation induced by 30 mg L−1 ferrous ion (Fe2+).

Toxoplasma gondii is one of the most common zoonotic protozoan parasites. It has three major infectious stages: rapidly multiplying tachyzoites (Tz), slowly replicating bradyzoites (Bz) and a resting/free-living stage, sporozoites (Sz). The regulatory mechanisms governing stage-specific gene expression are not fully understood. Few transcriptional start sites (TSS) are known for Sz. In this study, we obtained TSS of Sz using an oligo-capping method and RNA-seq analysis.

The antibiotic susceptibility patterns were correlated with particular spa types, and this correlation would help physicians select appropriate antibiotics for treatment of invasive MRSA infections. The aim of this research was to investigate invasive methicillin-resistant Staphylococcus aureus (MRSA) through delineatingthe molecular typing results and correlating them with antibiotic susceptibility.

Listeria monocytogenes causes listeriosis with a high mortality rate. This study systematically reviewed the antibiotic susceptibility of L. monocytogenes in the world. A literature search was done and the data of 33 studies that matched with the inclusion criteria, were used for meta-analysis. The random-effect model meta-analysis was applied to determine the frequency of overall L. monocytogenes and its antibiotic non-sensitive isolates.

Coastal zones are exposed to various anthropogenic impacts, such as different types of wastewater pollution, e.g., treated wastewater discharges, leakage from sewage systems, and agricultural and urban runoff. These various inputs can introduce allochthonous organic matter and microbes, including pathogens, into the coastal marine environment.

Advancements in contemporary medicine have led to an increasing life expectancy which has broadened the application of biomaterial implants. As each implant procedure has an innate risk of infection, the number of biomaterial-associated infections keeps rising. Staphylococcus aureus causes 34% of such infections and is known as a potent biofilm producer. By secreting micrococcal nuclease S. aureus is able to escape neutrophil extracellular traps by cleaving their DNA-backbone.

Toxoplasmosis is a major health issue worldwide especially for immune-deficient individuals and the offspring of newly infected mothers. It is caused by a unicellular intracellular parasite called Toxoplasma gondii. Although the drugs commonly used to treat toxoplasmosis are efficient, they present serious side effects and adverse events are common. Therefore, there is a need for the discovery of new compounds with potent anti-T. gondii activity.

Salmonella enterica serovar Typhimurium, with a broad-host range, is a predominant cause of non-typhoidal Salmonella infection in humans, and the infectious source is highly associated with food animals, especially poultry. Considering the horizontal transmission of S. Typhimurium from farm animals to humans, vaccination has been strongly recommended in industrial animals.

The in vitro antimicrobial activity of sophorolipids (SLs) against Eimeria maxima and Clostridium perfringens, and the in vivo effects of SLs on growth performance and gut health in necrotic enteritis (NE)-afflicted broiler chickens were studied.

Clostridium perfringens-induced necrotic enteritis (NE) is an economically important disease of broiler chickens. The present study evaluated the effect of C. perfringens on the intestinal histomorphometry, enteric microbial colonization, and host immune responses using 3 experimental NE reproduction methods.

The gastropod Rapana venosa is an edible mollusk suitable for biomonitoring of sea shelf contamination. In this work, the contents of five elements (Cu, Zn, As, Cd, Pb) in foot and in the rest of soft tissues of this mollusk were quantified, and the element contents in the edible foot were found below the maximum permissible levels set in several national and transnational regulations.

In order to explore the utilization of Eucalyptus sawdust (C) and develop its remediation potential in cadmium and arsenic contaminated soil, Eucalyptus sawdust were modified by FeCl3 and NaOH coprecipitation (MC). Characterization technology and pot experiment were used to explore the adsorption mechanism of cadmium and arsenic by MC and the effect of soil remediation.

Given the significance of food safety, it is highly urgent to develop a sensitive yet reliable sensor for the practical analysis of algal toxins. As most of the developed sensors are disturbed by interfering substances and the target toxin is detected in a single-signal manner based on the immunoassay technology.

Currently, no specific therapeutics are available for foodborne Shiga toxin-producing Escherichia coli (STEC) infections that cause severe gastroenteritis and life-threatening complications of hemolytic uremic syndrome (HUS). As STEC attachment to intestinal epithelium might increase the host absorption of Shiga toxins and severity of the disease, we were inspired to develop a bispecific neutralizer capable of blocking its Shiga toxin and adhesin intimin simultaneously.

by Woyinshet Worku, Moges Desta, Tadesse Menjetta Background Food-borne diseases related to the consumption of meat and its products had public health importance worldwide. The problem became worst in Ethiopia as the result of the tradition of eating raw cattle meat. Salmonella species and Escherichia coli are important food-borne pathogens associated with meat contamination.

Veterinary drug residues, heavy metals, and species authentication were performed on the demand of the regulatory agency in retail catfish fillets. A total of 362 domestic and imported catfish samples collected from various retail markets in the Northeast region of the United States were tested for the presence of chloramphenicol (CAP), malachite green/gentian violet (MG/GV), As, Cd, Pb, and Hg according to the USDA - FSIS protocols.

Listeria monocytogenes can grow under conditions at which fresh-cut fruit are stored, whereas Salmonella spp. has been associated with a number of outbreaks related to such products. It is therefore necessary to find products capable of reducing microbial counts while maintaining quality of the product. In this regard, ferulic acid (FA) has shown antimicrobial, antioxidant and many physiological functions in humans.

The behaviour of Listeria monocytogenes was investigated in soft pasteurized milk cheese elaborated with different salt concentrations (1.17 and 0.30% w/w) and in cured raw sheep milk cheese over storage up to 189 days at different isothermal conditions. Commercial 25-g cheese samples were inoculated with a 4-strain cocktail of L. monocytogenes (serovars 4b, 1/2a, 1/2b and 1/2c) at approximately 104 CFU/g.

Campylobacter hepaticus causes Spotty Liver Disease (SLD) in layer hens, resulting in mortality and productivity losses. Like other Campylobacter species, C. hepaticus is a fastidious organism that requires microaerobic conditions to grow and efficiently replicate. Despite its apparent vulnerability to environmental conditions, it is suspected that there are environmental sources of C.

Mycotoxins are secondary metabolites of fungi that cause severe damage to agricultural products and food in the food supply chain. These detrimental pollutants have been directly linked with poor socioeconomic patterns and human health issues. Among the natural micropollutants, ochratoxin A (OTA) and deoxynivalenol (DON) are widely distributed in food materials. The primary occurrence of these mycotoxins is reported in almost all cereal grains and fresh agro-products.

In the present study, a rapid and sensitive determination method of seven mycotoxins was developed using immunomagnetic solid-phase extraction (IMPSE) coupled with UHPLC-MS/MS. Monoclonal antibodies were conjugated with CNBr superparamagnetic beads, and the major parameters affecting the IMPSE efficiency were systematically investigated. Under the optimized conditions, the mycotoxins were purified with the IMSPE procedure within 15 min and simultaneously quantified by UHPLC-MS/MS.

Many factors affecting satellitism tests are unclear, and it is difficult to avoid misidentification, even if the medium is properly selected. We investigated the factors causing false-positive results for Haemophilus influenzae and false-negative results for Haemophilus parainfluenzae in the satellitism tests using Staphylococcus aureus as the source of nicotinamide adenine dinucleotide (NAD). H.

This study assessed the acid-adaptation of pathogenic and non-pathogenic strains of Escherichia coli in orange juice and the microbial resistance to the subsequent UV-C radiation treatment. Nine Shiga toxin-producing E. coli (STEC) and one strain of a non-pathogenic surrogate E. coli were used in this study. Each E.

Regarding the economic importance of bivalve farming, a great deal of interest has recently been devoted to studying the pathogenesis of infectious diseases of these mollusks to prepare for public health emergencies. Bacillus cereus is one of these pathogens; it is a ubiquitous soil bacterium responsible for many types of gastrointestinal diseases associated with food. This study was conducted to determine the pathogenic effect of B.

Journal of Food Science, EarlyView. Abstract The study aimed to evaluate the antimicrobial activity of 0.5 or 1% (w/w) chitosan and 1% (w/w) garlic against Salmonella spp., Escherichia coli O157:H7 and Listeria monocytogenes in hummus dip stored at 4, 10, or 25°C for 28, 21, or 7 days, respectively.