Research Publications (Food Safety)

Cryptosporidium is a common enteric parasite in chickens. A total of 812 fecal specimens were collected from 11 broiler farms in Zhejiang Province, China, and analyzed by nested PCR amplification based on the small subunit ribosomal RNA (SSU rRNA) gene. The overall infection rate of Cryptosporidium was 6.3% (51/812), and five of 11 farms were Cryptosporidium positive.

Sweet basil is a popular culinary herb used in many cuisines around the world and is widely grown commercially for retail as a live potted plant. However, basil is easily damaged by temperatures below 12 °C meaning plants must be transported from the grower to the retailer in a warm transport chain, adding considerable commercial cost in temperate countries.

Abstract Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the assessment of the application for renewal of Agal‐Pro BL/BL‐L®. The additive is a preparation of alpha‐galactosidase produced by a genetically modified strain of Saccharomyces cerevisiae (CBS 615.94) and endo‐1,4‐beta‐glucanase produced by a non‐genetically modified strain of Aspergillus niger (CBS 120604).

Novel modified atmosphere packaging (MAP) formulation maintains the qualities of strawberries. Abstract For fresh foods, the purpose and benefits of modified atmosphere packaging (MAP) generally include the preservation of antioxidants, the extension of shelf life, deferral of microbial spoilage, and retention of product color.

Biochar is an attractive and environmental friendly heavy metal sorbent, but is full-scale application is usually restricted by the limited sorption sites and surface area. Phosphate groups could strongly complex with heavy metal ions. Therefore, in the present study, phytic acid (PA) modified biochar (P-WBC) was prepared via the hydrolysis carbonization of wheat straw and PA at 300 °C and 700 °C.

Gelatin‐based encapsulation of Cinnamon EOs and bacteriocins is a practical application to extend the shelf‐life of meatballs. The antibacterial and antioxidant activity of encapsulated formulation has been evaluated. This new method can be used in industry for biopreservation of meat.

The TMA/TMAO pathway represents one of many microbe‐dependent pathways that will ultimately be linked to CHD pathogenesis Molecular docking results found that baicalein, fisetin, acacetin, and myricetin in flavonoid aglycones, and baicalin, naringin and hesperidin in flavonoid glycosides had good binding effects on TMA‐lyase, which were the most active and could be used as lead compounds for structural modification.

Abstract Background In an environment where the adoption of improved varieties resulting from plant breeding programs is limited, it is essential to identify end‐user preferences beforehand. A participatory survey was conducted in eight localities in Korhogo and Bouake regions, (central and northern Cote d'Ivoire, respectively) to identify producers' preferences and increase the adoption of improved varieties.

Staphylococcus aureus is one of the major foodborne pathogens contaminating milk and milk products. Isolates recovered from different stages in the cheese production chain and food processing environment (n = 16) in Poland were characterised using whole genome-sequencing and bioinformatic analyses. Most strains belonged to multi-locus sequence type (MLST) ST109 (6/37.5%) and ST130 (3/18.8%). Spa-typing of S.

A novel dual-signal fluorescent sensor was developed for detecting organophosphorus pesticides (OPs). It relies on the catalytic activities of acetylcholinesterase (AChE) and choline oxidase (ChOx) to generate hydrogen peroxide (H2O2) through the conversion of acetylcholine (ACh) to choline·H2O2 then oxidizes ferrocene-modified tetraphenylethylene (TPE-Fc) to its oxidized state (TPE-Fc+), resulting in enhanced cyan fluorescence due to aggregation.

Okadaic acid (OA), a marine biotoxin produced by microalgae, poses a significant threat to mariculture, seafood safety, and human health. The establishment of a novel, highly sensitive detection method for OA would have significant practical and scientific implications. Therefore, the purpose of this study was to develop an innovative approach for OA detection.

Hugan tablet is a Chinese medicine preparation. It is composed of Bupleuri Radix, Artemisiae Scopariae Herba, Isatidis Radix, Schisandrae Chinensis Fructus, Suis Fellis Pulvis, and Vigna radiata L. It has the effects of dispersing stagnated liver qi, strengthening the spleen and eliminating food to be used for the treatment of chronic hepatitis and early cirrhosis.

Summary Garlic peel extracts are known to have excellent physiological activity but research on the pre‐processing of garlic peel (GP) to enhance their bioactivity is limited.

A total DNA was extracted from a ‘mehovo sirene’ skin bag cheese, and subjected to amplicon‐based next‐generation sequencing. The raw data was bioinformatically processed to investigate the microbiome's alpha diversity. The Bulgarian ‘mehovo sirene’ cheese is a type of regional artisanal cheese prepared from raw ewe milk in a skin bag relying on spontaneous fermentation.

Route of contamination of raw milk and dairy products by Campylobacter spp., methods of detection and advantages of viability qPCR application to dairy matrices. The aim of this study was to investigate the survival of Campylobacter jejuni in dairy matrices by viability qPCR. Survival in UHT milk was examined first and the cheese outbreak strain, C. jejuni 11 218 showed enhanced tendency to become viable but nonculturable (VBNC).

Abstract The objective of this study is to evaluate the contamination by pesticides in food grown in the parish of Chugchilán. From 258 families that were part of the main project, a sub‐sample of 26 families was obtained and, for each of them, a sample of food was extracted and evaluated by chromatographic methods. The main pesticides found in the locality are from the group of organophosphates, carbamates, dithiocarbamates, and glyphosate.

Diagram for the development and characterization of biodegradable films based on gelatin/chitosan functionalized with eugenol and oregano essential oil and its application for the preservation of fresh cheese. Abstract The use of active substance‐containing biodegradable and edible coatings has become a potentially viable alternative for food preservation, especially to retard oxidative processes and the growth of pathogenic and spoilage microorganisms on the surface of the foodstuffs.

Effects of electrolyzed water‐ultrasound treatments on pesticide removal in fresh‐cut potatoes with different cutting methods. Abstract Electrolyzed water (EW) and ultrasound (US) treatment are emerging effective means of reducing pesticides in fresh‐cut vegetables. However, their synergetic efficacy of degrading pesticides in fresh‐cut potatoes has not yet been revealed.

Solar‐operated neera tapping device using Pelteir module as cooling system to collect neera in an effective manner without climbing the tree. Abstract The fresh sap harvested from the unbloomed inflorescence of the adult spadix of coconut tree (Cocus nucifera L.) is known as “neera.” It must be collected below room temperature to prevent naturally occurring fermentation.

Abstract Following a request from the European Commission, the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of a feed additive containing endo‐1,4‐β‐xylanase produced by Trichoderma citrinoviride DSM 34663 (Hostazym® X).

Abstract Background and Objectives High levels of deoxynivalenol (DON) contamination in foods can raise health risks for enterohepatic diseases. Here we report our investigation into the trichothecenes (DON, 3‐acetyl‐deoxynivalenol, 15‐acetyl‐deoxynivalenol), zearalenone (ZEA) and nivalenol (NIV) showing in the wheats‐processed noodles in Shandong, China (2019–2020).

Abstract Background Quinoa was suggested to substitute in noodles with positive effects of cardiovascular disease, metabolic, and gastrointestinal systems. However, quinoa fresh noodles (QFN) were hard to preserve but constrain industrial production. The deteriorative changes in the quality and relative parameters of QFN at 25 ± 1°C are lacking and needed to investigate.

Abstract Wild rats can host various zoonotic pathogens. Detection of these pathogens is commonly performed using molecular techniques targeting one or a few specific pathogens. However, this specific way of surveillance could lead to (emerging) zoonotic pathogens staying unnoticed. This problem may be overcome by using broader microbiome‐profiling techniques, which enable broad screening of a sample's bacterial or viral composition.

Abstract On August 2019 a staphylococcal food poisoning outbreak occurred in an elderly home in Piedmont, Italy. The epidemiological investigation performed among the persons that consumed the meal identified chicken salad as the most likely source of the outbreak.

Abstract Rattus norvegicus and Rattus rattus are commensal pest rodents, considered reservoirs and vectors of zoonotic pathogens. In livestock farms, the wide use of antimicrobials and their release into the environment lead to high long‐term residual concentrations, which may in turn lead to the occurrence of antimicrobial resistance (AMR).