Research Publications (Food Safety)

Ni and Cd are widely used together in the manufacture of cells and batteries. The incorrect disposal of these products can result in environmental contamination, posing risks to the organisms exposed to these contaminants. However, the effects of the mixture of Ni and Cd in freshwater fishes are still unclear in the current literature, especially in relation to biomarkers of oxidative stress.

The objective of this study was to use whole-genome sequencing (WGS) to screen for genes encoding for antibiotic resistance, fitness and virulence in Cronobacter sakazakii strains that had been isolated from food and powdered-milk-producing environments. Virulence (VGs) and antibiotic-resistance genes (ARGs) were detected with the Comprehensive Antibiotic Resistance Database (CARD) platform, ResFinder and PlasmidFinder tools. Susceptibility testing was performed using disk diffusion.

Staphylococcus aureus is a Gram-positive human opportunistic pathogen that may also cause food poisoning because of the ability of some strains to produce heat stable enterotoxins that can persist in foo

Campylobacter species are known to be able to produce biofilm, which represents an ideal protective environment for the maintenance of such fragile bacteria. Since the genetic mechanisms promoting biofilm formation are still poorly understood, in this study we assessed the ability of C. jejuni (n = 7) and C.

In this study, an activated charcoal-infused dental floss-based solid phase extraction procedure using as an efficient and easy–accessible carbon fiber adsorbent followed by dispersive liquid–liquid microextraction was introduced for the extraction and preconcentration of some widely used pesticides from fruit juices. The adsorbent was characterized by Fourier transform infrared spectrophotometry, scanning electron microscopy, and energy dispersive X-ray analysis.

Cadmium (Cd), commonly found in diet and drinking water, is known to be harmful to the human liver. Nevertheless, the effects and mechanisms of gestational Cd exposure on fetal liver development remain unclear.

The FDA Bacteriological Analytical Manual (BAM) Salmonella culture method takes at least 3 days for a presumptive positive result. The FDA developed a quantitative PCR (qPCR) method to detect Salmonella from 24-h preenriched cultures, using ABI 7500 PCR system. The qPCR method has been evaluated as a rapid screening method for a broad range of foods by single laboratory validation (SLV) studies.

In this study, a herd of Japanese Black (JB) breeding cattle with sporadic reproductive disorders was continuously monitored for an additional year to assess the effects of the urinary zearalenone (ZEN) concentration and changes in parameters (AMH and SAA) with time-lag variables and herd fertility (reproductive performance). This herd had high (exceeded the Japanese dietary feed regulations) urinary ZEN and rice straw ZEN concentrations (1.34 mg/kg).

The aim of this study was to determine the growth potential (δ) of Salmonella enterica in various parts (peel, stalk, pulp, and tip) of avocado and sugar apple at different maturation stages (green, mature, and ripe) over time. In addition, physicochemical parameters of the fruits were monitored using standard methods. There were statistically significant differences in the growth potential of S.

Vibrio anguillarum is a cause of vibriosis in marine fisheries worldwide, but only one previous study reported human pathogenicity of this species. Here, we report a 70-year-old man from Dalian, a coastal city in northeast China, who experienced a severe infection with V. anguillarum due to a bite on his left hand when handling hairtail, a marine fish. This patient had low immunity because of the long-term use of glucocorticoids due to nephrotic syndrome.

Journal of Fish Diseases, EarlyView. The spotted sea bass (Lateolabrax maculatus) is an economically important aquaculture fish species in East Asia. Because of its high nutritional value and delectable flavour, spotted sea bass has a high market demand (Chen et al., 2019).

Fish, like all other animals, are exposed to constant contact with microbes, both on their skin and on the surfaces of their respiratory and digestive systems. Fish have a system of non-specific immune responses that provides them with initial protection against infection and allows them to survive under normal conditions despite the presence of these potential invaders.

The Clostridium perfringens (C. perfringen) beta2 (CPB2) toxin produced by C. perfringens type C (CpC) can cause necrotizing enteritis in piglets. Immune system activation in response to inflammation and pathogen infection is aided by long non-coding RNAs (lncRNAs). In our previous work, we revealed the differential expression of the novel lncRNA LNC_001186 in CpC-infected ileum versus healthy piglets.

Journal of Food Safety, EarlyView. Ultrafine bubbles (UFB) are a novel concept that has the potential to enhance the potency of antimicrobials to eliminate biofilms.

by Hao-Ching Wang, Shin-Jen Lin, Han-Ching Wang, Ramya Kumar, Phuoc Thien Le, Jiann-Horng Leu Photorhabdus insect-related toxins A and B (PirA and PirB) were first recognized as insecticidal toxins from Photorhabdus luminescens. However, subsequent studies showed that their homologs from Vibrio parahaemolyticus also play critical roles in the pathogenesis of acute hepatopancreatic necrosis disease (AHPND) in shrimps.

Bovine milk is a low cost, high nutritional value product commercialised worldwide. To maintain high standard levels and preserve consumer health, several tests could be used to identify antimicrobial drug residues, run voluntarily or officially planned and performed, according to European legislative requirements.

The prevalence and resistance of C. jejuni in small ruminants in Jordan to antimicrobials of human clinical importance and the management factors that associate with C. jejuni in the flocks were determined. Flock-level prevalence of C. jejuni was 28.5%; individual animals’ prevalence was 11.0%.

Aflatoxin, with higher toxicity, is widely found in grains such as peanut and corn. This study proposes an unsupervised learning method to detect aflatoxin based on deep spectral features.

It is important to understand the distribution of strains of Bacillus cereus that produce the emetic toxin, cereulide, in raw milk, and to evaluate their impact on the safety of milk and dairy products. The distribution of cereulide-producing B. cereus was investigated for isolates from raw milk in Hokkaido, Japan, and the ability of strains to produce cereulide in milk was also studied.

The objective of this study was to examine the frequency and extent of antibiotic-resistant pathogens in seafood sold in Malaysia, using a systematic review and meta-analysis approach to analyze primary research studies. Four bibliographic databases were systematically searched for primary studies on occurrence. Meta-analysis using a random-effect model was used to understand the prevalence of antibiotic-resistant bacteria in retail seafood sold in Malaysia.

In this study, based on the high-throughput automatic sample pretreatment with immunoaffinity magnetic beads with oriented immobilized antibodies, grain and feed fumonisin (FB) content was detected using pre-column automatic derivatization of high-performance liquid chromatography (HPLC). The FB capacity of well-oriented antibody immunoaffinity magnetic beads was 1.5−1.8 times that of magnetic beads with randomly fixed antibody.

Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens causing severe diseases. The ability of STEC to produce disease is associated with Shiga toxin (Stx) production. We investigated the occurrence of STEC on bovine and pork carcasses and walls of trucks where they were transported, and we characterized virulence genes and serotypes of STEC strains.

In the present study, we aimed to evaluate the concentration and health risk of aflatoxin B1 (AFB1), heavy metals (HMs), and phthalate acid esters (PAEs) in peanut samples in Shandong Province, China, and 255 peanut samples were collected from 20 counties in 2020.

Salmonella is a poultry-associated pathogen that is considered one of the most important zoonotic bacterial agents of contaminated food of animal origin including poultry products. Many efforts are taken to eliminate it from the food chain, and phages are one of the most promising tools to control Salmonella in poultry production. We investigated the usefulness of the UPWr_S134 phage cocktail in reducing Salmonella in broiler chickens.

Salmonella is a food-borne pathogen often linked to poultry sources, causing gastrointestinal infections in humans, with the numbers of multidrug resistant (MDR) isolates increasing globally.