Research Publications (Food Safety)

The Global Antimicrobial Resistance Surveillance System (GLASS) is one of the pillars of the global action plan on antimicrobial resistance launched by the World Health Organization in 2015. This study was conducted to determine the feasibility and benefits of GLASS as a component of antimicrobial stewardship strategies in three provincial hospitals in Thailand.

Antimicrobial drug resistance is a major health issue plaguing healthcare worldwide and leading to hundreds of thousands of deaths globally each year. Tackling this problem requires discovery and development of new antibacterial agents.

Outbreaks and recalls associated with foods containing spices suggest a need for risk assessment of Salmonella in spices. In this study, the survivability of Salmonella Enteritidis PT 30, Salmonella cocktail (S. Enteritidis PT 30, S. Tennessee K4643 and S. Agona 447967) and Enterococcus faecium NRRL B-2354 in chili, cinnamon and black pepper at water activities (aw) 0.3 and 0.5 were evaluated during one-year storage at 21 °C.

Listeria monocytogenes (LM) is a bacterial pathogen known to cause rhombencephalitis in ruminants. Neutrophils accumulate intracerebrally as microabscesses, where bacterial phagocytosis occurs. In-situ investigations in ruminant brains indicate that LM can persist in neutrophils, but LM interaction with neutrophils is poorly studied.

Environmental Microbiology Reports, EarlyView. Antibiotic resistance (AR) in bacteria is an urgent and global health issue, encompassing clinical, agricultural, terrestrial and aquatic environments. AR is not only expressed through genetic resistance. It is also found in bacteria in a small fraction of populations exhibiting antibiotic ‘persister’ states, thereby acting as a reservoir for re-growth.

Journal of Applied Microbiology, Accepted Article. This study explored how dairy farm operating systems with free-stall or tie-stall housing and cow hygiene score influence the occurrence of zoonotic bacteria in raw milk. Samples from bulk tank milk, milk filters, feces, feed, teats and teat milk were collected from eleven farms with loose housing and seven farms with tie-stall housing every second month over a period of 11 months and analyzed for the presence of STEC by culturing c

International Journal of Food Science & Technology, EarlyView. A new gelatin-protocatechuic acid (PCA) film with Escherichia coli O157:H7 phage JN01 was developed and characterised. After incorporated with JN01, swelling value, water vapour permeability, water solubility and elongation at break of gelatin-PCA film were not significantly different.

Salmonellosis involving chicken meat is one of the most frequent foodborne diseases registered worldwide. Many studies report the prevalence of Salmonella spp. on chicken meat; however, data are limited or variable.

Salmonella enterica is a leading human pathogen responsible for foodborne outbreaks worldwide. In the last decade, foods with low water activity (aw) and high-fat content have been involved in an increased occurrence of foodborne outbreaks. This research focuses on the foodstuff tahini, which is often linked to Salmonella infection outbreaks and recalls.

This study presents epidemiological data on the prevalence of Salmonella spp. and E. coli in bivalve molluscs marketed in Sardinia (Italy). E. coli enumeration and Salmonella spp. occurrence at batch level were used to verify the association between microbiological food safety criteria in place in the European Community.

Frozen reformulated (FR) breaded chicken products have previously been implicated in causing human salmonellosis. A multi-country Salmonella enterica serovar Enteritidis outbreak involving several strains with >400 reported human cases in the UK occurred in 2020. Initially S. Infantis was detected in one sample from a case home but S. Enteritidis was then also isolated using a S.

Catecholamine stress hormones (norepinephrine, epinephrine, and dopamine) are signals that have been shown to be used as environmental cues, which affect the growth and virulence of normal microbiota as well as pathogenic bacteria. It has been reported that Escherichia coli and Salmonella use the two-component system proteins QseC and QseE to recognise catecholamines and so act as bacterial adrenergic receptors. In this study, we mutated the E.

Listeriosis is an emerging bacterial disease of animals and humans worldwide, caused by Listeria monocytogenes. The infected dairy cows continuously shed the microbes in their milk, a human being’s concern. This study was designed to molecular characterize the Listeria monocytogenes isolated from symptomatic cow's milk of tehsils Samundri, Gujar khan, and Alipur of Punjab. A total of 175 milk samples were collected, pre-enriched and cultured on PALCAM agar.

Clostridium perfringens is one of the highest prevailing spore-forming foodborne pathogens, which is widely distributed and causes severe disease and outbreaks in humans and animals. Raw meat and poultry are the main vehicles of this pathogen. In this study, we investigated the prevalence, antibiotic resistance pattern, toxin-encoding genes and genetic diversity of C.

Transboundary and Emerging Diseases, Accepted Article. Staphylococcus aureus is a pathogen that can affect multiple host species. Evidence of transmission between humans and animals and among different animal species has been reported in recent years. In this study, we investigated 284 free-living red deer (Cervus elaphus) in the Central Italian Alps to assess the prevalence and molecular characteristics of S.

Journal of Food Safety, EarlyView. Foodborne illness outbreaks caused by consuming cantaloupe melon contaminated with Salmonella spp. and Escherichia coli O157:H7 are recurrent. The objective of this work was to investigate the antimicrobial effect of the combination of chitosan (Q) and extracellular metabolites of Pediococcus pentosaceus CM175 (EmPp) on the surface of cantaloupe against E.

There have been reports of patients suffering from typhoid fever, particularly those involving infants and immunocompromised patients, which at times present with Salmonella induced brain infection. Although rare, it has frequently been associated with adverse neurological complications and increased mortality.

Adequate surrogate identification is critical for validating in-plant thermal process controls for Salmonella inactivation in different food matrices. This study compared the thermal inactivation parameters (D- and z-values) and evaluated the heat resistance of Enterococcus faecium (8459) as a surrogate for a 5-serovar Salmonella cocktail in cornmeal.

The objective of this work is to match available phylogenetic information for Bacillus cereus strains with published thermal resistance parameters (D90°C, z) and to use this information to develop refined inactivation models for B. cereus sensu lato. To do so, the thermal resistance parameters were retrieved for 57 strains of B. cereus that could be assigned to a phylogenetic group.

This study investigated the bactericidal effects of plasma-activated acetic acid (PAAA) on Salmonella Typhimurium and its impact on the physicochemical traits of chicken meat. Twenty milliliters of 0.8% (v/v) acetic acid (AA) were treated with plasma (2.2 kHz and 8.4 kVpp) for 30 min. The chicken skins, breasts, and drumsticks, inoculated with S. Typhimurium, were immersed in AA or PAAA and incubated for 10 min.

Salmonella enterica serovar Typhimurium (S. Typhimurium) continues to be a serious concern to the poultry industry as a bacterial food-borne zoonosis, which generally results in intestinal inflammation and barrier dysfunction or even death. Eugenol is a phenolic compound with various pharmacological activities involved antioxidant, anti-inflammatory and antibacterial effects, which is expected to be an effective non-antibiotic therapy.

To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.

Different feeding regimens in infancy alter the gastrointestinal (gut) microbial environment. The fecal microbiota in turn influences gastrointestinal homeostasis including metabolism, immune function, and extra-/intra-intestinal signaling.

Eurotium cristatum is a potential probiotic fungus that is used to enhance Fuzhuan tea quality through fermentation and could reduce obesity by modulating gut dysbiosis. This study aimed to investigate the effects and possible mechanisms of killed E. cristatum (KEC) and its polysaccharides (ECP) in ulcerative colitis (UC) relief. KEC and ECP were administered to mice with dextran sulfate sodium-induced UC.

Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) in poultry is most often transmitted by the fecal–oral route, which can be attributed to high population density. Upon encountering the innate immune response in a host, the pathogen triggers a stress response and virulence factors to help it survive in the host.