Research Publications (Food Safety)

The rising demand for minimally processed, natural, and healthier food products has led to the search for alternative and multifunctional bioactive food components. Therefore, the present study focuses on the functional proprieties of a peptide fraction derived from Saccharomyces cerevisiae metabolism.

This study investigated the combined effect of Ultraviolet (UV) light-emitting diode (LED) technology treatment with refrigerated storage of chicken breast meat over 7 days on Campylobacter jejuni, Salmonella enterica serovar Typhimurium, total viable counts (TVC)

Gram-negative Vibrio species are major foodborne pathogens often associated with seafood intake that causes gastroenteritis. On food surfaces, biofilm formation by Vibrio species enhances the resistance of bacteria to disinfectants and antimicrobial agents. Hence, an efficient antibacterial and antibiofilm approach is urgently required. This study examined the antibacterial and antivirulence effects of chromones and their 26 derivatives against V. parahaemolyticus and V. harveyi.

Abstract A supplemental effect of Achyranthes aspera extract (200 mg/kg phytosterol) in diets was evaluated for Pacific white shrimp (Penaeus vannamei) on growth performance, digestibility, innate immunity, antioxidant capacity, and disease resistance against Vibrio parahaemolyticus. A fish meal and soybean meal‐based control diet (Con; protein 38.0% and lipid 8.7%) was formulated and five other diets were prepared by adding A.

Cattle are recognized as the principal reservoir for Escherichia coli O157:H7 and preharvest food safety efforts often focus on decreasing shedding of this pathogen in cattle feces. Enogen® corn (EC; Syngenta Seeds, LLC) is genetically modified to produce enhanced concentrations of α-amylase in the corn kernel endosperm.

Salmonella is one of the most prevalent foodborne pathogens in poultry and its products. Its rapid detection based on volatile organic compounds (VOC) has been widely accepted. However, the variation in the VOCs of Salmonella-contaminated chicken during the early stage (48 h) remains uncertain.

Gelatin‐based encapsulation of Cinnamon EOs and bacteriocins is a practical application to extend the shelf‐life of meatballs. The antibacterial and antioxidant activity of encapsulated formulation has been evaluated. This new method can be used in industry for biopreservation of meat.

Staphylococcus aureus is one of the major foodborne pathogens contaminating milk and milk products. Isolates recovered from different stages in the cheese production chain and food processing environment (n = 16) in Poland were characterised using whole genome-sequencing and bioinformatic analyses. Most strains belonged to multi-locus sequence type (MLST) ST109 (6/37.5%) and ST130 (3/18.8%). Spa-typing of S.

Summary Garlic peel extracts are known to have excellent physiological activity but research on the pre‐processing of garlic peel (GP) to enhance their bioactivity is limited.

A total DNA was extracted from a ‘mehovo sirene’ skin bag cheese, and subjected to amplicon‐based next‐generation sequencing. The raw data was bioinformatically processed to investigate the microbiome's alpha diversity. The Bulgarian ‘mehovo sirene’ cheese is a type of regional artisanal cheese prepared from raw ewe milk in a skin bag relying on spontaneous fermentation.

Route of contamination of raw milk and dairy products by Campylobacter spp., methods of detection and advantages of viability qPCR application to dairy matrices. The aim of this study was to investigate the survival of Campylobacter jejuni in dairy matrices by viability qPCR. Survival in UHT milk was examined first and the cheese outbreak strain, C. jejuni 11 218 showed enhanced tendency to become viable but nonculturable (VBNC).

Diagram for the development and characterization of biodegradable films based on gelatin/chitosan functionalized with eugenol and oregano essential oil and its application for the preservation of fresh cheese. Abstract The use of active substance‐containing biodegradable and edible coatings has become a potentially viable alternative for food preservation, especially to retard oxidative processes and the growth of pathogenic and spoilage microorganisms on the surface of the foodstuffs.

Abstract Background Quinoa was suggested to substitute in noodles with positive effects of cardiovascular disease, metabolic, and gastrointestinal systems. However, quinoa fresh noodles (QFN) were hard to preserve but constrain industrial production. The deteriorative changes in the quality and relative parameters of QFN at 25 ± 1°C are lacking and needed to investigate.

Abstract On August 2019 a staphylococcal food poisoning outbreak occurred in an elderly home in Piedmont, Italy. The epidemiological investigation performed among the persons that consumed the meal identified chicken salad as the most likely source of the outbreak.

Abstract Rattus norvegicus and Rattus rattus are commensal pest rodents, considered reservoirs and vectors of zoonotic pathogens. In livestock farms, the wide use of antimicrobials and their release into the environment lead to high long‐term residual concentrations, which may in turn lead to the occurrence of antimicrobial resistance (AMR).

Abstract The incidence of salmonellosis, and other enteric zoonotic diseases, has been associated with various food and environmental exposures and socioeconomic factors. Increasingly, there is interest in exploring differences among serotypes of Salmonella to better inform public health prevention efforts.

Abstract Establishing efficient methods to combat bacterial biofilms is a major concern. Natural compounds, such as essential oils derived from plants, are among the favored and recommended strategies for combatting bacteria and their biofilm. Therefore, we evaluated the antibiofilm properties of peppermint oil as well as the activities by which it kills bacteria generally and particularly their biofilms.

Abstract The aim of the present study was to prepare a new antimicrobial Pickering emulsion of which the star anise essential oil was added to the oil phase, and to investigate the effect of stabilization by bio‐based active nanoparticles consisting of zein and pectin loaded with thymol.

Abstract Antibiotic drugs (ABD) are often used in shrimp farms to treat the disease when a bacterial diagnosis is presumed. This scenario was the impetus to carry out a field survey to define how ABD are employed in the State of Sonora, the second largest producer of shrimp in Mexico. Twenty‐nine farms were surveyed through their general managers.

Abstract An acute infectious disease of northern sheatfish (Silurus soldatovi) was found in a commercial aquaculture pond in Tianjin, China. Herein, two dominant bacterial strains were isolated from naturally diseased northern sheatfish and identified as Aeromonas veronii and Vibrio cholerae. Seven healthy and seven naturally diseased fish that were collected from the pond were used for the gut microbiota analysis by high‐throughput 16S rRNA gene sequencing.

Abstract The study aimed to evaluate the effect of tributyrin on the growth performance, survival, hypoxia tolerance, and nutrient digestibility of Pacific white shrimp fed 25% and 50% soybean meal (SBM) diets. Six experimental diets were formulated: (1) 25% SBM without tributyrin; (2) 25% SBM with 0.05% tributyrin; (3) 25% SBM with 0.1% tributyrin; (4) 50% SBM without tributyrin; (5) 50% SBM with 0.05% tributyrin; and (6) 50% SBM with 0.1% tributyrin.

Abstract Vibrio parahaemolyticus is a significant causal agent of acute hepatopancreatic necrosis disease (AHPND), with huge production losses of white leg shrimp, Litopenaeus vannamei, cultivated globally, including aquaculture farms in the Mekong Delta of Vietnam. Controlling this disease is critical because of the worldwide expansion of antimicrobial‐resistant V. parahaemolyticus isolates. The purpose of this study was to determine the frequency of multi‐antibiotic resistance (MAR) in V.

Abstract Soybean meal (SBM) is widely used in shrimp feed agro‐industry as a fish meal (FM) replacement. However, high levels of SBM may compromise animal health as a result of nutrient imbalance.

This paper investigates how the rise in ambient temperature designed according to climate change affects the attachment levels of Escherichia coli O157:H7, O104:H4 and O26 on cress leaves. This study revealed that temperature increase designed according to climate change did not cause any significant change in persistence of pathogens studied here on leaf tissue.

Fatty acid amides of palmitic (PAPY), lauric (LAPY), myristic (MYPY), and decanoic (DEPY) acids inhibited bacteria. Significant inhibition of Listeria and other bacteria increased at higher amide concentrations (10,000–20,000 ppm). LAPY reduced Listeria growth by 1.55 to >5.0 Logs in co‐inoculation assays and by 0.51 to >3.00 Log CFU/g on produce. Abstract Foodborne pathogens are a persistent threat to food and consumer safety.