Research Publications (Food Safety)

The present study evaluated the effects of carvacrol and thymol against Salmonella spp. biofilm on polypropylene. The efficacy of the compounds was assessed by quantifying Salmonella spp. cells during and after biofilm formation on polypropylene and performing scanning electron microscopy. During biofilm formation, carvacrol and thymol, at subinhibitory concentrations, reduced bacterial counts about 1–2 log, while established Salmonella spp.

Acute hepatopancreatic necrosis disease (AHPND) is a severe, newly emergent penaeid shrimp disease caused by Vibrio parahaemolyticus that has already led to tremendous losses in the cultured shrimp industry. Until now, its disease-causing mechanism has remained unclear. Here we show that an AHPND-causing strain of V. parahaemolyticus contains a 70-kbp...

Publication date: February 2016
, Volume 53, Part B
Author(s): Eun-Jeong Hong, Dong-Hyun Kang

Elizabeth B. Norton, Luis M. Branco, John D. Clements

Publication date: January 2016
Source:LWT - Food Science and Technology, Volume 65
Author(s): Dongying Hu, Haixia Wang, Lijuan Wang

The aim of this study was to evaluate the impact of nisin with lauryl arginine ester (LAE) or their combination by 1: 3 (w/w) on cheese preservation. The study was carried out against the most common foodborne pathogens in recombined feta cheese (RFC) and processed cheese spread (PCS).

Publication date: February 2016
, Volume 60
Author(s): R.F.A. Lokerse, K.A. Maslowska-Corker, L.C. van de Wardt, T. Wijtzes

Publication date: February 2016
, Volume 60
Author(s): A. Rodríguez, R. Gordillo, M.J. Andrade, J.J. Córdoba, M. Rodríguez

Publication date: February 2016
, Volume 60
Author(s): Bjørn Tore Lunestad, Sylvia Frantzen, Cecilie Smith Svanevik, Irja Sunde Roiha, Arne Duinker

Marinades containing polyphosphates have been previously implicated in the enhanced survival of Campylobacter spp. in poultry product exudates. The enhanced Campylobacter survival has been attributed primarily to the ability of some polyphosphates to change the pH of the exudate to one more amenable to Campylobacter.

Campylobacter jejuni (C. jejuni) is a leading cause of human bacterial gastroenteritis worldwide. Previous research has shown that circadian rhythm plays a critical role in host response to C. jejuni colonization. The CLOCK gene is one of the core genes regulating circadian rhythms and shows significant expression on 7 d post-C. jejuni inoculation. The objective of this study was to investigate temporal and spatial expression of chicken CLOCK gene post-C. jejuni inoculation.

The present study was carried out to investigate the effect of dietary supplementation of different levels of mannan-oligosaccharides (MOS) on performance, egg quality, immune responses, and gut microflora in laying hens exposed to Escherichia coli (E. coli) challenge. A total of 180 Hy-Line W-36 laying hens, 55 wk of age, were randomly distributed among 5 dietary treatments with 6 replicates of 6 hens each.

This study investigates the effects of different doses of serotonin, its precursor 5-hydroxytry-ptophan (5HTP), and m-hydroxybenzylhydrazine inhibitor (NSD1015), administered via intraperitoneal for 5 consecutive days, on behavior and average body weight of broilers. We also measured the humoral immune response and quantification of Salmonella Enteritidis in broilers chickens that received the drugs evaluated and a Lactobacillus pool.

In order to reduce the risk of enteric pathogens transmission in animal farms, the disinfection effectiveness of slightly acidic electrolyzed water (SAEW, pH 5.85 to 6.53) for inactivating Salmonella Enteritidis on the surface of plastic poultry transport cages was evaluated. The coupled effects of the tap water cleaning time (5 to 15 s), SAEW treatment time (20 to 40 s), and available chlorine concentrations (ACCs) of 30 to 70 mg/l on the reductions of S.

After a ban on the use of antibiotics as growth promoters in farm animals in the European Union in 2006, an interest in alternative products with antibacterial or anti-inflammatory properties has increased. In this study, we therefore tested the effects of extracts from Curcuma longa and Scutellaria baicalensis used as feed additives against cecal inflammation induced by heat stress or Salmonella Enteritidis (S. Enteritidis) infection in chickens.

Publication date: December 2015
, Volume 110
Author(s): S.M. Osés, A.M. Diez, E.M. Gómez, D. Wilches-Pérez, P.A. Luning, I. Jaime, J. Rovira

Publication date: 1 March 2016
, Volume 194
Author(s): Tai-Yuan Chen, Shu-Hao Kuo, Shui-Tein Chen, Deng-Fwu Hwang

Vibrio cholerae is an aquatic organism and facultative human pathogen that colonizes the small intestine. In the small intestine V. cholerae is exposed to a variety of antimicrobial compounds including bile. V. cholerae resistance to bile is multifactorial and includes alterations in the membrane permeability barrier that are mediated by ToxR; a membrane-associated transcription factor.

The ubiquitous bacterial second messenger c-di-GMP has recently become prominent as a trigger for biofilm formation in many bacteria. It is generated by diguanylate cyclases (DGC, with GGDEF domains) and degraded by specific phosphodiesterases (PDE, containing either EAL or HD-GYP domains). Most bacterial species contain multiples of these proteins with some having specific functions that are based on direct molecular interactions in addition to their enzymatic activities. E.

Maja Šikić Pogačar, Anja Klančnik, Franz Bucar, Tomaž Langerholc, Sonja Smole Možina

The response of some inoculated strains and native flora to PL treatment (Xenon lamp, 3 pulses s−1, 10 cm distance from the lamp, 71.6 J cm−2) in apple, orange and strawberry fresh juices with different absorbance, turbidity and particle size was investigated. Microbial growth dynamics during 12-day storage (5 °C) of PL-treated juices was also evaluated. PL treatments provoked 0.3–2.6 log reductions for inoculated microorganisms and 0.1–0.7 for native flora.

Despite the putative endemic status of swine influenza A virus (swIAV) infections, data on the occurrence of swine influenza outbreaks are scarce in Brazil. The aim of this study was to detect and subtype swIAVs from six outbreaks of porcine respiratory disease complex (PRDC) in southern Brazil. Nasal swabs were collected from 66 piglets with signs of respiratory disease in six herds. Lung tissue samples were collected from six necropsied animals.

Publication date: January 2016
Source:LWT - Food Science and Technology, Volume 65
Author(s): Ji-Yeon Lee, Dong-Hyun Kang

Publication date: January 2016
Source:LWT - Food Science and Technology, Volume 65
Author(s): María J. Martelo-Vidal, Inmaculada C. Fernández-No, Esther Guerra-Rodríguez, Manuel Vázquez

Publication date: January 2016
Source:LWT - Food Science and Technology, Volume 65
Author(s): Estefanía Muñoz-Atienza, Carlos Araújo, Rosa del Campo, Pablo E. Hernández, Carmen Herranz, Luis M. Cintas