Research Publications (Food Safety)

Publication date: January 2016
Source:LWT - Food Science and Technology, Volume 65
Author(s): Agapi I. Doulgeraki, Maria Papaioannou, George-John E. Nychas

Rod-shaped bacterial cells are polarized, with many organelles confined to a polar cellular site. In polar flagellates, FlhF and FlhG, a multiple-domain (B-N-G) GTPase and a MinD-like ATPase respectively, function as a cognate pair to regulate flagellar localization and number as revealed in Vibrio and Pseudomonas species.

Publication date: Available online 22 August 2015

Author(s): Anja Klančnik, Nataša Toplak, Minka Kovač, Hélène Marquis, Barbara Jeršek

Aims
This study aimed to evaluate the efficiency of four phosphate-solubilizing bacteria (PSB) on the growth and yield of rice under different soil conditions.

Aims
The aim of this study was to cost-effectively improve detection of foodborne pathogens in PCR inhibitory samples through the use of alternative DNA polymerases.

Methicillin-resistant Staphylococcus aureus (MRSA) presents major health risk for humans causing serious nosocomial and community acquired infections. Asymptomatic food-producing animal carriers and their meat may represent potential reservoirs for human infections. The aim of the present study was to investigate the prevalence of MRSA in small ruminants raised under free-range conditions and their meat at slaughter and retail level in Northern Greece.

Publication date: Available online 21 August 2015

Author(s): Régis Tuchtenhagen Sturbelle, Luciana Farias da Costa de Avila, Talita Bandeira Roos, Jessica Lopes Borchardt, Rita de Cassia Conceição, Odir Antonio Dellagostin, Fábio Pereira Leivas Leite

Publication date: Available online 21 August 2015

Author(s): Angelika Fruth, Rita Prager, Erhard Tietze, Wolfgang Rabsch, Antje Flieger

Publication date: Available online 21 August 2015

Author(s): Annette Jurke, N. Bannert, K. Brehm, V. Fingerle, V.A.J. Kempf, D. Kömpf, M. Lunemann, A. Mayer-Scholl, M. Niedrig, K. Nöckler, H. Scholz, W. Splettstoesser, D. Tappe, Silke F. Fischer

Pore-forming toxins (PFTs) are a distinct class of membrane-damaging cytolytic proteins that contribute significantly towards the virulence processes employed by various pathogenic bacteria. Vibrio cholerae cytolysin (VCC) is a prominent member of the beta-barrel PFT (beta-PFT) family. It is secreted by most of the pathogenic strains of the intestinal pathogen V. cholerae. Owing to its potent membrane-damaging cell-killing activity, VCC is believed to play critical roles in V.

Foodborne Pathogens and Disease , Vol. 0, No. 0.

Foodborne Pathogens and Disease , Vol. 0, No. 0.

The human pathogen Vibrio vulnificus is the leading cause of seafood-related deaths in the United States. Strains are genotyped on the basis of alleles that correlate with isolation source, with clinical (C)-genotype strains being more often implicated in disease and environmental (E)-genotype strains being more frequently isolated from oysters and estuarine waters. Previously, we have shown that the ecologically distinct C- and E-genotype strains of V.

Pathogenic enteric viruses are responsible for a wide range of infections in humans, with diverse symptoms. Raw and partially treated wastewaters are major sources of environmental contamination with enteric viruses.

The control of multihost pathogens, such as Coxiella burnetii, should rely on accurate information about the roles played by the main hosts. We aimed to determine the involvement of the red deer (Cervus elaphus) in the ecology of C. burnetii. We predicted that red deer populations from broad geographic areas within a European context would be exposed to C. burnetii, and therefore, we hypothesized that a series of factors would modulate the exposure of red deer to C. burnetii.

Phytophagous insects can encounter Salmonella enterica on contaminated plant surfaces and transmit externally adhered and internalized bacteria on and among leaves. Excretion of ingested S. enterica by the leafhopper Macrosteles quadrilineatus has been previously reported; however, the sites of persistence of ingested bacteria remain undetermined. Fluorescence microscopy revealed the presence and persistence of S. enterica in various organs of M.

Publication date: Available online 20 August 2015

Author(s): Wilson J. Bertu, Marie J. Ducrotoy, Pilar M. Muñoz, Virginie Mick, Amaia Zúñiga-Ripa, Ward Bryssinckx, Jacob K.P. Kwaga, Junaid Kabir, Susan C. Welburn, Ignacio Moriyón, Reuben A. Ocholi

The estuarine gram-negative rod and human diarrheal pathogen Vibrio cholerae synthesizes a VPS exopolysaccharide-dependent biofilm matrix that allows it to form a 3D structure on surfaces. Proteins associated with the matrix include, RbmA, RbmC, and Bap1. RbmA, a protein whose crystallographic structure suggests two binding surfaces, associates with cells...

Natural transformation is one mechanism of horizontal gene transfer (HGT) in Vibrio cholerae, the causative agent of cholera. Recently, it was found that V. cholerae isolates from the Haiti outbreak were poorly transformed by this mechanism. Here, we show that an integrating conjugative element (ICE)-encoded DNase, which we name...

H. M. Abdallah, E. A. Reuland, B. B. Wintermans, N. al Naiemi, A. Koek, A. M. Abdelwahab, A. M. Ammar, A. A. Mohamed, C. M. J. E. Vandenbroucke-Grauls

Objectives

The aim of the present study was to determine the prevalence and to characterize extended-spectrum β-lactamases- and/or carbapenemases-producing Enterobacteriaceae among Enterobacteriaceae isolated from retail chicken meat in Zagazig, Egypt.

Methods

Kirk S. B. Bergstrom, Vijay Morampudi, Justin M. Chan, Ganive Bhinder, Jennifer Lau, Hyungjun Yang, Caixia Ma, Tina Huang, Natasha Ryz, Ho Pan Sham, Maryam Zarepour, Colby Zaph, David Artis, Meera Nair, Bruce A. Vallance

Aims
Ileocaecal lymph nodes (ICLNs) of pigs, the key immune inductive site for bacterial systemic invasion, were examined in this study with emphasis on viable and cultivable bacteria.

A quantitative comparison between discriminatory indexes and concordance among multilocus variable-number tandem-repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), automated ribotyping, and phage typing has been performed, testing 238 Salmonella enterica serotype Enteritidis isolates not epidemiologically correlated.

Whole-genome sequencing (WGS) has emerged today as an ultimate typing tool to characterize Listeria monocytogenes outbreaks. However, data analysis and interlaboratory comparability of WGS data are still challenging for most public health laboratories. Therefore, we have developed and evaluated a new L. monocytogenes typing scheme based on genome-wide gene-by-gene comparisons (core genome multilocus the sequence typing [cgMLST]) to allow for a unique typing nomenclature.

We isolated Shiga toxin-producing Escherichia coli O157:H7 strains resistant to third-generation cephalosporins. The resistant strains harbored blaCMY-2, a plasmid-mediated AmpC β-lactamase. Genotyping of isolates revealed the possible spread of this problematic bacterium. Results suggested the importance of the investigation and surveillance of enterobacteria with plasmids harboring blaCMY-2.