Research Publications (Food Safety)

BACKGROUND The development of brewer's spent grain protein films with potential active packaging properties was investigated. Films were prepared by casting protein dispersions at different pHs (2, 8, 11), plasticizers (polyethylene glycol –PEG– or glycerol), and levels (0–0.25 g g−1) of PEG. Mechanical, water‐barrier and solubility, optical, antioxidant (reducing power, ABTS•+ and lipidic radical scavenging), and antimicrobial properties of films were determined.

Author(s): Zekun Jin, Li Li, Yin Zheng, Peipei An

Bacterial toxins are food safety hazards causing about 10% of all reported foodborne outbreaks in Europe. Pertinent to Gram‐positive pathogens, the most relevant toxins are emetic toxin and diarrheal enterotoxins of Bacillus cereus, neurotoxins of Clostridium botulinum, enterotoxin of Clostridium perfringens, and a family of enterotoxins produced by Staphylococcus aureus and some other staphylococci.

High moisture slurries used in production of table spreads may permit growth of  Staphylococcus aureus and  Bacillus cereus  and subsequent production of heat stable enterotoxin. Compliance with the Food Safety Modernization Act (FSMA), specifically 21 CFR Part 117, Subpart B and Section 117.8 (c)(2) and (c)(3) requires a hazard analysis to determine if preventive controls are needed. In this study, growth of  S. aureus  and  B.

ABSTRACT

The present study was conducted to administer a commercial multi‐strain probiotic (MP), Yilibao (Bacillus velezensis:Bacillus cereus:Lactobacillus casei = 2:2:1), in Nile tilapia, Oreochromis niloticus. In terms of aerobic Bacillus spp. counts, we produced five diets containing 0, 0.34, 1.68, 3.36 and 6.72 g/kg of MP dry product (Control, T1, T2, T3, T4).

Background Human-use probiotics have recently been associated with clinical infections and antibiotic resistance transfer, raising public concern over their safety. However, despite their extensive application in aquaculture and animal husbandry, the safety of animal-use probiotics remains poorly described. Methods We evaluated the safety of 92 animal-use probiotics from China.

The occurrence of multidrug-resistant pathogenic bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant Acinetobacter baumannii (MDRAB), extended-spectrum β-lactamase (ESBL) Escherichia coli, and Pseudomonas aeruginosa, has become a serious problem in animals and public.

Streptomyces is the most frequently described genus of Actinomycetes, a producer of biologically active secondary metabolites. Indeed, the Streptomyces species produces about 70% of antibiotics and 60% of antifungal molecules used in agriculture. Our study was carried out with the goal of isolating and identifying antimicrobial secondary metabolites from Streptomyces misionensisV16R3Y1 isolated from the date palm rhizosphere (southern Tunisia).

Bacillus cereus spores pose a challenge to the food industry owing to their ability to germinate in food during storage and produce toxins. The objective of the current study was to investigate the effect of pulsed electric field (PEF) at low electric field strength (i.e., 7.3, 8.1, 8.8 and 9.4 kV/cm) and moderate temperatures (65, 70, 75 and 80°C) on B. cereus spores.

Bacillus cereus sensu lato comprises Gram-positive spore-forming bacteria producing toxins associated with foodborne diseases. Three pore-forming enterotoxins, nonhemolytic enterotoxin (Nhe), hemolysin BL (Hbl), and cytotoxin K (CytK), are considered the primary factors in B. cereus sensu lato diarrhea. The aim of this study was to determine the potential risk of enterotoxicity among soil B.

Aims The objective of this study was to evaluate the antimicrobial effects of Radio frequency (RF) heating and the combination treatment of RF heating with ultraviolet (UV) radiation against foodborne pathogens in roasted grain powder (RGP). Methods and Results Foodborne pathogens inoculated on RGP were subjected to RF heating or RF‐UV combination treatments. After 120 s of RF heating, 4.68, 3.89, and 4.54 log reductions were observed for E. coli, S. Typhimurium, and B.

Some biological activities of dihydroquercetin (DHQ), including antioxidative, antibacterial and anticancer activities, and the oxidative stability of butter oil supplemented with DHQ were evaluated. The DPPH* radical‐scavenging activity was high for DHQ, with an IC50 of 63.83 ± 3.11 μg/ml, compared to that of Trolox, which had an IC50 of 117.02 ± 5.19 μg/ml, over the concentrations studied (10–100 μg/ml).

BACKGROUND Jiuqu are vital saccharifying and fermenting agents for Chinese fermented foods. Natural ventilation during Jiuqu fermentation causes changes in temperature, oxygen and moisture content, resulting in mass and heat gradients from the outer to inner areas of Jiuqu blocks. In the present study, microbiota stratification in Jiuqu was investigated by single molecule real‐time sequencing and culture isolation.

Author(s): Anuj H. Chheda , Madhavi R. Vernekar An indigenously isolated Bacillus cereus isolated from soil was found to be capable of producing ε-poly-l-lysine (ε-PL), a homo-poly-amino acid of l-lysine which is used as a safe food preservative. The production of the ε-PL was optimized by identifying the most significant components of the medium which affect its production under shake-flask conditions.

In this study, antagonistic effects of the chitinolytic enzyme producing isolates of Pseudomonas fluorescens PB27, Bacillus cereus B1 and Bacillus thuringiensis K1 against the aflatoxigenic fungus Aspergillus flavus were investigated. Germination of the spores of A. flavus was inhibited by up to nearly 20% after co-incubation with P. fluorescens PB27 for 72h, whereas the fungal spores were less affected when incubated with either B. cereus B1 or B. thuringiensis K1. When P.

Pepper bacterial wilt is caused by the bacterial pathogen, Ralstonia solanacearum. It is the most destructive disease of many Solanaceous crops such as potatoes, tobacco, pepper, tomatoes, and eggplant and is a significant source of crop loss worldwide. Physical, cultural and chemical controls have been employed to combat this destructive disease.

In dairy plants, clean-in-place (CIP) apparatus cannot be disassembled and, therefore, it is not easy to clean the inner surface of their pipes. In this study, the inhibitory effects of chemical agents on biofilms formed by three foodborne pathogens, Bacillus cereus , Escherichia coli , and Staphylococcus aureus , were evaluated in a dairy CIP system.

This study examined the microbicidal activity of 222-nm UV radiation (UV222), which is potentially a safer alternative to the 254-nm UV radiation (UV254) that is often used for surface decontamination. Spores and/or growing and stationary-phase cells of Bacillus cereus, Bacillus subtilis, Bacillus thuringiensis, Staphylococcus aureus, and Clostridioides difficile and a herpesvirus were all killed or inactivated by UV222 and at lower fluences than with UV254. B.

Anti‐digestive enzyme, antioxidant, anti‐proliferative and antibacterial activities of the extract from Moringa oleifera at different extraction temperatures. Summary In an ultrasonic bath and for 30 min in the presence of water as extraction solvent in a ratio of 1:10 (g/mL), effect of extraction temperature on extraction of caffeoylquinic acids (CQAs) from Moringa oleifera leaves (MOL) was investigated.

Rapid identification from positive blood cultures is standard of care (SOC) in many clinical microbiology laboratories. The GenMark Dx ePlex Blood Culture Identification Gram-Positive (BCID-GP) Panel is a multiplex nucleic acid amplification assay based on competitive DNA hybridization and electrochemical detection using eSensor technology.

Inactivation of Bacillales and Clostridiales spores is of interest, since some cause food spoilage and human diseases. A recent publication (Schlievert et al. 2018) reported that glycerol monolaurate (GML) in a non‐aqueous gel (GMLg) effectively killed spores of Bacillus subtilis, Bacillus cereus, and Clostridioides difficile, and Bacillus anthracis spores somewhat We now show that: 1) the B.

The microbiological quality of ready-to-eat (RTE) foods from school cafeterias in Chongqing, China, was evaluated in comparison to a guideline published by a provincial health commission.

Author(s): J. Rocha-Pimienta, S. Martillanes, R. Ramírez, J. Garcia-Parra, J. Delgado-Adamez

Microorganisms in dairy industries can form monospecies, dual-species, or multispecies biofilms, showing cooperative or competitive behaviors, which might contribute to the reduction of efficiency of cleaning and sanitization processes and eventually turn into a potential source of contamination. This study proposes to evaluate the behavior of Listeria monocytogenes in monospecies biofilms, cocultured with Bacillus cereus.