Research Publications (Food Safety)

The antimicrobial and antioxidant effects of Citrus aurantium L. flowers (Bahar narang) extract (BE; 500, 1,000 and 2,000 ppm) were studied in traditional yoghurt stew during storage at 4C for 28 days. The contents of total phenolics and flavonoids in BE were 81 ± 1.8 and 46 ± 2.4 mg/g, respectively. In vitro antibacterial activity of BE showed that BE had a strong antimicrobial activity against Escherichia coli O157:H7, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus.

The aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of Mentha spicata plant against common food-borne pathogenic bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Listeria monocytogenes, Salmonella typhimurium, and Escherichia coli O157:H7). Chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (GC-MS).

Hasmik Hayrapetyan, Marcel Tempelaars, Masja Nierop Groot, Tjakko Abee

Improper cooling of cooked rice at an inappropriate temperature or leaving cooked rice at room temperature can cause food poisoning attributed to Bacillus cereus. Natural food preservative of either squid or crab polymer chitosan solution was added to examine their antibacterial properties against Bacillus cereus in cooked rice during storage at 37 and 4 °C.

Publication date: 15 October 2015
, Volume 211
Author(s): Laure Pujol, Nicholas Brian Johnson, Catherine Magras, Isabelle Albert, Jeanne-Marie Membré

Publication date: 15 October 2015
, Volume 211
Author(s): Virginie Castiaux, Xiaojin Liu, Laurence Delbrassinne, Jacques Mahillon

Publication date: 15 October 2015
, Volume 211
Author(s): Adelson Alves de Oliveira Junior, Hyrla Grazielle Silva de Araújo Couto, Ana Andréa Teixeira Barbosa, Marcelo Augusto Guitierrez Carnelossi, Tatiana Rodrigues de Moura

Aims
To isolate bacteria from soil for the purpose of microbial pre-treatment of brown crab (Cancer pagurus) shell waste and the production of chitin.

Preservative agents determining the shelf life of cosmetic products must have effective antimicrobial activity while meeting safety requirements for topical use. In this study, we determined the antimicrobial activity of 1,2-hexanediol against several Gram-positive and Gram-negative bacteria. Antimicrobial susceptibility tests have shown that 1,2-hexanediol exhibits broad-spectrum activity against Gram-positive and Gram-negative bacteria with MICs of 0.5 – 2% (v v−1).

The aim of this study was to evaluate the performance of immunodetection methods and PCR analysis of enterotoxigenic Bacillus cereus strains. Methods and Results Eighty-eight enterotoxigenic B. cereus group strains linked to foodborne outbreaks and illnesses were studied with 30 exclusivity non-enterotoxigenic strains including B. amyoliquifaciens, B. subtilis, S. aureus, Salmonella, and E. coli for this assessment.

Publication date: March 2015
, Volume 101
Author(s): Lei Wang , Peng Jin , Jing Wang , Lulu Jiang , Shurong Zhang , Hansheng Gong , Hongxia Liu , Yonghua Zheng

A single laboratory validation study of a rapid and sensitive quantitative method for the analysis of cereulide toxin produced by Bacillus cereus using ultra high performance liquid chromatography-electrospray-tandem mass spectrometry is presented. The analysis of this cyclic peptide toxin was validated for pasta and rice samples using a newly presented synthetic cereulide peptide standard, together with 13C6-cereulide that previously have not been commercially available.

Publication date: February 2015
, Volume 100
Author(s): I. Leceta , S. Molinaro , P. Guerrero , J.P. Kerry , K. de la Caba

Publication date: 2 March 2015
, Volume 196
Author(s): Line Thorsen , Christine Kere Kando , Hagrétou Sawadogo , Nadja Larsen , Bréhima Diawara , Georges Anicet Ouédraogo , Niels Bohse Hendriksen , Lene Jespersen

When exposed to nutrient or non-nutrient germinants, individual Bacillus spores can return to life through germination followed by outgrowth. Laser tweezers Raman spectroscopy, and either differential interference contrast or phase contrast microscopy were used to analyze the slow dipicolinic acid (DPA) leakage (normally ~20% of spore DPA) from individual spores that takes place prior to the lag time, Tlag, when spores begin rapid release of remaining DPA.

Publication date: 16 March 2015
, Volume 197
Author(s): Hue Luu-Thi , Jorinde Corthouts , Ioannis Passaris , Tara Grauwet , Abram Aertsen , Marc Hendrickx , Chris W. Michiels

Publication date: 16 March 2015
, Volume 197
Author(s): Palmira De Bellis , Fiorenza Minervini , Mariaelena Di Biase , Francesca Valerio , Paola Lavermicocca , Angelo Sisto