Research Publications (Food Safety)

Journal of Applied Microbiology, Accepted Article. Aim This study was done to determine the effects of temperature, pH, and sodium chloride (NaCl) on antimicrobial activity of magnesium oxide (MgO) nanoparticles (NPs) against Escherichia coli O157:H7. Methods and Results Culture conditions were established by varying the pH (5.0, 7.2, and 9.0), NaCl concentration (0.5, 2.0, 3.5, and 5.0%, w/v), and incubation temperatures (4, 12, 22, and 37°C).

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1467-1478, February 2022. Aims The biological events occurring during human digestion help to understand the mechanisms underlying the dose–response relationships of enteric bacterial pathogens.

Journal of Applied Microbiology, Volume 132, Issue 1, Page 290-297, January 2022. Aims In this study, the efficacy of using vacuumed hydrogen peroxide vapour (VHPV) to inactivate foodborne pathogens in whole dried black pepper (Piper nigrum) and powdered dried red pepper (Capsicum annuum) was evaluated. Methods and Results Black and red pepper inoculated with Escherichia coli O157:H7 and Salmonella Typhimurium were su

Journal of Applied Microbiology, Accepted Article. Abstract
Aims
The biological events occurring during human digestion help understand the mechanisms underlying the dose-response relationships of enteric bacterial pathogens. To better understand these events, we investigated the growth and reduction behavior of bacterial pathogens in an in vitro model simulating the environment of the small intestine.

Journal of Applied Microbiology, Accepted Article. ABSTRACT
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In this study, the efficacy of using vacuumed hydrogen peroxide vapor (VHPV) to inactivate foodborne pathogens in whole dried black pepper (Piper nigrum) and powdered dried red pepper (Capsicum annuum) was evaluated.

This research investigated the influence of soil microbiota on E. coli O157:H7 survival in soil rinse and artificial soil. Additionally, the influence of selected soil bacteria on E. coli O157:H7 in soil environments was determined.

This study evaluated the effects of Bacillus amyloliquefaciens (B. amyloliquefaciens) TL106 isolated from Tibetan pigs’ feces, on the growth performance, immune response, intestinal barrier function, morphology of jejunum, cecum and colon, and gut microbiota in the mice with enterohemorrhagic Escherichia coli (EHEC)‐induced intestinal diseases.

Aims To analyze the non‐glycosylated protein fraction from Melipona beecheii honey for antimicrobial activity against Escherichia coli O157:H7. Methods and Results The proteins from M. beecheii honey were separated according to their degree of glycosylation using Concanavalin A‐affinity chromatography. The total protein extract and its fractions were analyzed by 1D and 2D electrophoresis.

Aims To identify and evaluate the application of two novel monoclonal antibody (mAb) 2G12 against outer membrane protein (Omp) C and mAb 12B1 targeting the O chain of the lipopolysaccharides (LPS) of Escherichia coli O157:H7 (ECO157). Methods and Results The sensitivity and specificity of these two antibodies were evaluated with eight ECO157 strains and 68 untargeted strains.

Aim To study the impact of incorporating micro‐nano‐bubbles (MNBs) in commonly used food antimicrobials (AMs) against Escherichia coli O157:H7 (EC) and Listeria monocytogenes (LM). Methods and Results Air, carbon dioxide (CO2) and nitrogen (N2) were used to incorporate MNBs in city water.

Aims The objective of the study was to determine levels of Escherichia coli O157:H7 colonization in the gastrointestinal tract (GIT) of naturally‐shedding cattle shedding the pathogen at low‐ or super‐shedder levels. Methods and Results Over two years, feedlot cattle were sampled multiple times for faecal shedding of E. coli O157:H7.

Aims To evaluate tomato epiphyte lactic acid bacteria (LAB) hydrophobicity and auto‐aggregation as an indicator of bacteria adhesion to tomato. Likewise use LAB adhesion and co‐aggregation as mechanisms to antagonize pathogen attachment. Methods and Results Fifty‐four LAB were screened to evaluate their hydrophobic, auto‐ and co‐aggregative properties against Salmonella Typhimurium, Saintpaul, Montevideo and Escherichia coli O157:H7.

Aims The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy post‐weaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). Methods and Results Out of the 128 isolates, L. plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. L.

Aims To investigate the effects of ohmic heating (OH) and water bath heating (WB) on the membrane permeability, membrane structure, intracellular organization and leakage of intracellular substances of Escherichia coli O157:H7 at the same inactivation level and at a heating temperature of 72°C. Methods and Results Flow cytometry analysis indicated that membrane permeability of E. coli O157:H7 by OH was comparable to WB at 72°C.

Aims The aim of this study was to evaluate the microbiological quality of commercially prepared RTE sushi by enumerating aerobic mesophilic bacteria (AMB) and thermotolerant coliforms (TC) and detecting Escherichia coli and Salmonella spp. An isolate was identified as E. coli O157:H7 which was evaluated for its virulence and antimicrobial resistance profiling as well as its ability to form biofilms on stainless steel.

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To investigate the effects of ohmic heating (OH) and water bath heating (WB) on the membrane permeability, membrane structure, intracellular organization, and leakage of intracellular substances of E. coli O157:H7 at the same inactivation level and heating temperature of 72 °C.

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Greenhouse trials were conducted with different cultivars of baby leaf spinach, rocket, and Swiss chard and inoculation of Escherichia coli O157:H7 gfp+, to determine whether plant species and cultivar has an impact on establishment of this strain.

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The purpose of this research was to determine optimum conditions for broth recovery of heat‐injured Salmonella Typhimurium and Escherichia coli O157:H7.

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Molecular subtyping is commonly used in foodborne disease surveillance and microbial source tracking. There is a knowledge gap regarding the molecular ecology of foodborne pathogens in non-food associated environments. The objective of this study was to isolate and subtype foodborne pathogens from pristine natural environments with minimal anthropogenic inputs.

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Enterohaemorrhagic Escherichia coli (EHEC) serotype O157:H7 as a major human pathogen is responsible for food borne outbreaks, bloody diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS) and even death. In this study, the antibacterial activity of the Zataria multiflora essential oil (ZMEO) and nanoliposome-encapsulated ZMEO was evaluated on the pathogenicity of Escherichia coli O157:H7.

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In this study, the effects of addition of salt to treatment with acids (one of several organic acids and salt in various solutions including rich or minimal broth, buffer, or distilled water) on the reduction of Escherichia coli O157:H7 were investigated. The protein expression profiles corresponding to acid stress (acetic acid) with or without salt addition were studied using a comparative proteomic analysis of E. coli O157:H7.

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The objective of this study was to determine if the fecal microbiome has an association with E. coli O157:H7 prevalence and enumeration.

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The objective of this work was to study the growth potential of E. coli O157:H7 and Salmonella spp. in leafy vegetable extracts at different temperature conditions.

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The aim of the present study was to examine the effects of caprylic acid (CA) on pathogens in apple juice having intrinsic organic acids, and to determine any synergistic effects.

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The aim of this study was to study inactivation of different pathogenic bacteria on agar model surface using TiO2-UV photocatalysis (TUVP).