Research Publications (Food Safety)

Journal of Applied Microbiology, Accepted Article. Aims The aim of the current study was to investigate the effect of plasma-mediated oxidative stress on the post-treatment viability of Listeria monocytogenes at the physiological and molecular levels. Methods and Results 107 CFU/mL L. monocytogenes in 10 mL phosphate-buffered saline (PBS) were treated with atmospheric nonthermal plasma for 0, 30, 60, 90 and 120 s, respectively.

Journal of Applied Microbiology, Accepted Article. To isolate the slow-growing or viable but non-culturable competitive exclusion (CE) microorganisms from composts and then verify the anti-Listeria monocytogenes activities of those CE isolates in compost. CE strains were isolated from composts using double- or triple-layer agar methods, purified, and then characterized. Both compost extracts and solid compost samples were spiked with a cocktail of 3 L.

Journal of Applied Microbiology, Accepted Article. This study explored how dairy farm operating systems with free-stall or tie-stall housing and cow hygiene score influence the occurrence of zoonotic bacteria in raw milk. Samples from bulk tank milk, milk filters, feces, feed, teats and teat milk were collected from eleven farms with loose housing and seven farms with tie-stall housing every second month over a period of 11 months and analyzed for the presence of STEC by culturing c

Journal of Applied Microbiology, Volume 132, Issue 3, Page 1866-1876, March 2022. Aims This study aimed to evaluate the effectiveness of selected essential oils (EOs) and hydrolates (Hs) against Listeria monocytogenes biofilms on polystyrene (PS) and stainless steel (SS) surfaces. Methods and Results Among others, Origanum hirtum EO, Corydothymus capitatus EO and Citrus aurantium H were selected to

Journal of Applied Microbiology, Accepted Article. Aims To reveal the antibacterial mechanism of the combination of thymol and cinnamaldehyde to Listeria monocytogenes ATCC 19115 on autoclaved chicken breast. Methods and Results In this study, Listeria monocytogenes ATCC 19115 on autoclaved chicken breast was exposed to the stress of 125 μg/mL thymol and 125 μg/mL cinnamaldehyde, and transcriptome analysis was used to reveal the crucial ant

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1518-1525, February 2022. Aim The study aimed to investigate the role of cattle slaughterhouse wastewater as a possible source for the environmental distribution of Listeria monocytogenes. Methods and Results Listeria spp. isolation was performed by collecting 117 wastewater samples from four different cattle slaughterhouses in Turkey.

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1467-1478, February 2022. Aims The biological events occurring during human digestion help to understand the mechanisms underlying the dose–response relationships of enteric bacterial pathogens.

Journal of Applied Microbiology, Accepted Article. Aims
This study aimed to evaluate the effectiveness of selected essential oils (EOs) and hydrolates (Hs) against L. monocytogenes biofilms on polystyrene (PS) and stainless steel (SS) surfaces.

Journal of Applied Microbiology, Accepted Article. Abstract
Aims
The biological events occurring during human digestion help understand the mechanisms underlying the dose-response relationships of enteric bacterial pathogens. To better understand these events, we investigated the growth and reduction behavior of bacterial pathogens in an in vitro model simulating the environment of the small intestine.

Journal of Applied Microbiology, Accepted Article. Aim
The study aimed to investigate the role of cattle slaughterhouse wastewater as a possible source for the environmental distribution of Listeria monocytogenes.

Journal of Applied Microbiology, Accepted Article. The purpose of this study was to detect growth enhancing or inhibiting activity between bacterial populations from raw milk under different conditions (temperature, medium).

Aims In this study, benzalkonium chloride (BAC) microcapsules were developed for surface disinfection purpose and were evaluated against Listeria monocytogenes and Escherichia coli biofilms. Methods and Results Microcapsules were prepared with two different strategies: uncomplexed BAC‐microcapsules (UBM), containing BAC and maltodextrins and complexed BAC‐microcapsules (CBM) containing BAC complexed by pectin and maltodextrins.

Aims To investigate the effects of the lectin from Punica granatum sarcotesta (PgTeL) on growth, viability, cell structure, biofilm formation and chitinase activity of Listeria monocytogenes. In addition, the effect of PgTeL on the adhesion and invasion of human cells (HeLa) was determined.

Aims This study evaluated the microbiological quality and safety of minimally processed parsley sold in southeastern Brazilian food markets. Methods and Results One hundred samples were submitted to the enumeration of Enterobacteriaceae by plating on MacConkey agar. Colonies of Enterobacteriaceae were randomly selected and identified by MALDI‐TOF MS. Samples were also tested for Listeria monocytogenes and Salmonella spp.

Aim To study the impact of incorporating micro‐nano‐bubbles (MNBs) in commonly used food antimicrobials (AMs) against Escherichia coli O157:H7 (EC) and Listeria monocytogenes (LM). Methods and Results Air, carbon dioxide (CO2) and nitrogen (N2) were used to incorporate MNBs in city water.

Aims To understand the genetics involved in surface attachment and biofilm formation of Listeria monocytogenes. Methods and Results An in vitro screen of a Himar1 transposon library of L. monocytogenes strain 15G01 identified three transposants that produced significantly different biofilm levels when compared to the wild‐type strain; two mutants exhibited enhanced biofilm formation and one produced less biofilm biomass than the wild‐type.

Aim of the study Effect of internalized phthalyl starch nanoparticles (PSNs) on the antimicrobial ability of Lactococcus lactis (LL) KCTC 2013.

ABSTRACT Aims This study measured the survival of Listeria monocytogenes and Salmonella enterica subsp. enterica serovar Javiana over a 10–day period on four soil‐free cultivation matrix (SFCM) types in the absence of microgreens and fertilizers. Methods and Results Coco coir (CC), a Sphagnum peat/vermiculite mix, Biostrate®, and hemp mat samples were inoculated with 3 x 106 CFU mL‐1 bacteria, incubated at room temperature, and analyzed on day 0, 1, 3, 6, and 10.

Aims The aim of this study was to test the efficacy of new and currently used biocides in the mushroom industry for inactivating L. monocytogenes biofilm. Methods and results A lab‐scale study was initially carried out to test the efficacy of eleven biocidal products against a cocktail of five L. monocytogenes strains that were grown to three‐day biofilms on stainless steel coupons. Biocidal efficacy was then tested under clean and dirty conditions based on the EN 13697:2015 method.

Aims The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy post‐weaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). Methods and Results Out of the 128 isolates, L. plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. L.

Aims Exposure of Listeria monocytogenes to osmotic stress can induce increased resistance to subsequent lethal exposure to cell envelope stressors, such as nisin and bile salts. We wanted to determine if similar cross‐protection phenotypes could occur when L. monocytogenes strains were treated with osmotic stress and exposed to sub‐lethal levels of the cell envelope stressor, bile. Method and Results Growth phenotypes were measured for six L.

Aims To study the antimicrobial agents of the Bacillus velezensis strain HC6 and assess the application potential of Bacillus velezensis HC6 in maize. Methods and Results We applied a dual culture technique to test the antimicrobial activity of B. velezensis HC6 against bacteria and fungi of common contaminated crops. B. velezensis HC6 showed antagonistic action on pathogenic fungi, including Aspergillus and Fusarium, as well as pathogenic bacteria (especially Listeria monocytogenes).

Aims The aim of this study was to develop a rapid detection and differentiation method for pathogenic Listeria species in stone fruits. Methods and Results We utilized activated charcoal enrichment media (ACM) to induce overexpression and hypersecretion of pathogenic Listeria virulence proteins which can subsequently be detected via immunoblot analysis. Plum and nectarine slices spiked with either L. monocytogenes or L.

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An extensive source investigation was conducted on a dairy farm with neurolisteriosis and subclinical mastitis cases to identify infection source and potential transmission routes of Listeria monocytogenes.

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The control of Listeria monocytogenes biofilm formation using lactocin AL705 bacteriocin at sub‐minimum inhibitory concentrations (MICs) through an antiquorum sensing strategy, was preliminarily investigated.