Research Publications (Food Safety)

Author(s): Fangshen Guo, Fangyuan Wang, Haiyan Ma, Zhouzheng Ren, Xiaojun Yang, Xin Yang

Author(s): Mauricio Redondo-Solano, Carol Valenzuela-Martinez, Vijay K. Juneja, Dennis E. Burson, Harshavardhan Thippareddi

ABSTRACT

Author(s): Dongryeoul Bae, Jeong-Woo Lee, Jong-Pyo Chae, Jae-Won Kim, Jong-Su Eun, Kyung-Woo Lee, Kun-Ho Seo

Clostridium perfringens poses a serious threat to small ruminants by causing moderate to severe enterotoxaemia. Due to its ability to produce a wide arsenal of toxins, it is ranked among the most prevalent and important pathogens in livestock. This study focused on the molecular characterization of different Clostridium perfringens types along with their antimicrobial resistance profile. An overall higher prevalence of C.

Author(s): Lucilla Iacumin, Giuseppe Comi

Clostridium (C.) perfringens is the causative agent of several diseases and enteric infections in animals and humans. The pathogenicity of the bacterium is largely mediated by the production of a wide range of toxins. Individual C. perfringens strains produce only subsets of this toxin repertoire, which permits the classification in seven toxinotypes (A–G). In addition, a variety of minor toxins further characterizes the single strains.

Author(s): Xinheng Zhang, Qiqi Zhao, Xiaotong Ci, Sheng Chen, Zi Xie, Hongxin Li, Huanmin Zhang, Feng Chen, Qingmei Xie

Author(s): Jingjing Sun, Cheng Zhang, Bingkun Zhang

ABSTRACT Aims This study aimed to determine the toxin genotypes, virulence determinants, and antibiogram of Clostridium perfringens isolated from poultry, animals, and humans. Biofilm formation and the efficacy of disinfectants on C. perfringens biofilms were studied. Methods and Results Thirty C. perfringens isolates (20 clinical and 10 from chicken carcasses) were genotyped by PCR and all isolates were genotype A (cpa+).

In Japan, strategies for ensuring food safety have been developed without reliable scientific evidence on the relation between foodborne infections and foods. The aim of this research was to provide information on the proportions of foodborne diseases caused by seven major causative pathogens ( Campylobacte r spp., Salmonella spp., EHEC, V. parahaemolyticus , Clostridium perfringens , Staphylococcus aureus , and Norovirus) attributable to foods using analysis of outbreak surveillance data.

Timely and accurate methods for detecting Clostridium perfringens-associated diseases (CPAD) are crucial to improve patient care. A number of studies have evaluated the accuracy of nucleic acid amplification tests (NAAT) in detecting CPAD, but decisive results about their effectiveness have not been reported. We conducted a meta-analysis to evaluate the diagnostic performance of NAAT for detecting C. perfringens in clinical diarrheal samples.

Author(s): Baikui Wang, Altaf Hussain, Yuanhao Zhou, Zihan Zeng, Qi Wang, Peng Zou, Li Gong, Pengwei Zhao, Weifen Li

Aim The aim of the present study was to examine the vaccine immune response in ewes supplemented with Bacillus toyonensis BCT‐7112T during a period of 5‐day supplementation before vaccination against a recombinant Clostridium perfringens epsilon toxin (rETX). Methods and Results Ewes were vaccinated with 200 µg of rETX adjuvanted with 10% aluminum hydroxide. The treat group was orally supplemented with B.

This study investigated the effects of dietary Bifidobacterium bifidum (BFD) and mannan‐oligosaccharide (MOS), as a synbiotic, on the production performance, gut microbiology, serum biochemistry, antioxidant profile, and health indices of broiler chicken.

Author(s): Zahra H. Mohammad, Larry Payton, Sujata A. Sirsat

In this work, the fermented chili pastes of normal samples (NS) and abnormal samples (ANS, aerogenic samples) were analyzed. The gas produced in the ANS was carbon dioxide. The kinds of bacteria genus were the same in both samples. However, concentrations of Bacillus and Clostridium in ANS were higher than those in NS. This finding indicated they might be the potential bacteria for gas production.

Author(s): Yanhua Wang

Gas gangrene, caused mainly by the anaerobic bacterium Clostridium perfringens (C. perfringens), causes death within 48 h of onset. Limited therapeutic strategies are available, and it is associated with extremely high mortality. Both C. perfringens alpha toxin (CPA) and perfringolysin O (PFO) are important virulence factors in the development of gas gangrene, suggesting that they are therapeutic targets.

Author(s): Li Xiu, Yu Liu, Wei Wu, Suo Chen, Zhaobing Zhong, Hairong Wang

The Florida Complaint and Outbreak Reporting System (FL-CORS) database is used by the Florida Department of Health’s Food and Waterborne Disease Program (FWDP) as one of the tools to detect foodborne disease outbreaks. We present a descriptive and spatial network analysis of the FL-CORS data collected during 2015-2018.

ABSTRACT

Bacterial toxins are food safety hazards causing about 10% of all reported foodborne outbreaks in Europe. Pertinent to Gram‐positive pathogens, the most relevant toxins are emetic toxin and diarrheal enterotoxins of Bacillus cereus, neurotoxins of Clostridium botulinum, enterotoxin of Clostridium perfringens, and a family of enterotoxins produced by Staphylococcus aureus and some other staphylococci.

To identify the free‐living amoeba (FLA) and amoeba resistant bacteria (ARB) accumulated in zebra mussels and in the water in which they are found, mussels were collected at two locations in the Ebro river basin (North East Spain). Free‐living amoebas and bacteria were isolated from mussel extracts and from natural water.

Here, we report the complete genome sequence of Clostridium perfringens 2016TE7641_69, isolated from the intestine of a turkey reared in a conventional poultry flock located in central Italy, where animals were showing enteric disorders suggesting subclinical necrotic enteritis.