Research Publications (Food Safety)

Salmonella enterica subsp. enterica Typhimurium and its monophasic variant I 1;4,[5],12:i:- (MVST) are responsible for thousands of reported cases of salmonellosis each year in Canada, and countries worldwide. We investigated S.

A total of 55 food and clinical S. Schwarzengrund isolates were assayed for plasmid content, among which an IncFIB-IncFIC(FII) fusion plasmid, conferring streptomycin resistance, was detected in 17 isolates. Among the 17 isolates, 9 were food isolates primarily collected from poultry meat, and 8 clinical isolates collected from stool, urine, and gallbladder.

The spread of superbugs in dairy products can jeopardize global public health. To date, information on the incidence rates of virulent and β-lactams-resistant (BLR) Salmonella in cheeses from rural areas of Egypt has been lacking. Biochemical, serological, antibiotic susceptibility, and multiplex PCR (M-PCR) tests were performed to identify and characterize Salmonella isolates.

Rapid growth in commercial poultry production is one of the major sources of Salmonella infections that leads to human salmonellosis. The two main Salmonella enterica serovars associated with human salmonellosis are enteritidis and typhimurium. The aim of this study was to determine the prevalence of S. enterica serovars Enteritidis and S. Typhimurium as well as their Salmonella pathogenicity islands (SPI) and antibiotic resistance profiles in broiler chicken feces from slaughterhouses.

Dust sprinkling was an effective way to experimentally colonise layer chickens with Salmonella Typhimurium. Salmonella infection with 1000 CFU/g of dust altered the gut microbiota. This model mimics Salmonella infection in the field. Abstract Role of dust in Salmonella transmission on chicken farms is not well characterised.

This research underscored the crucial role of Alcalase and Flavourzyme in generating antibacterial peptides. Certain fractions of mung bean and adzuki bean protein hydrolysates contained bioactive peptides against S. aureus and S. Typhimurium. Interestingly, small cationic peptides containing lysine with an amphipathic nature were a key factor in inhibiting S. aureus.

The microbial quality and safety on two types of unpasteurised juices in Lleida was evaluated. No Salmonella spp., L. monocytogenes, or E. coli were detected. Summary The growing consumption of untreated juices has increased the outbreaks related to Salmonella spp., Escherichia coli O157:H7 and Listeria monocytogenes. Although these pathogens are not in favourable conditions in fruit juices, cases of survival in acidic environments have been reported in fruit and vegetable juices.

The graphical abstract presents a detailed analysis of data from the RASFF concerning herbs and spices. On the left, various herbs and spices are depicted, representing the subjects of the notifications. Below this, a pie chart categorizes the types of RASFF notifications, with border rejections constituting the majority. On the right, the graphic lists the top 10 notifying countries, countries of origin, and notified products, with chili being the most frequently reported product.

Decontamination of egg‐associated pathogens using plasma‐activated water and plasma‐activated hydrogen peroxide, along with examining their characteristics and effects on egg quality. Abstract This study investigated the effectiveness of plasma‐activated water (PAW) and plasma‐activated hydrogen peroxide (PAHP) in reducing egg‐associated pathogens.

Endolysins are bacteriophage (or phage)-encoded enzymes that catalyse the peptidoglycan breakdown in the bacterial cell wall. The exogenous action of recombinant phage endolysins against Gram-positive organisms has been extensively studied. However, the outer membrane acts as a physical barrier when considering the use of recombinant endolysins to combat Gram-negative bacteria.

Applied and Environmental Microbiology, Ahead of Print. Salmonella Typhimurium is a zoonotic pathogen that poses a major threat to public health. This generalist serotype can be found in many hosts and the environment where varying selection pressures may result in the accumulation of antimicrobial resistance determinants.

by Aliyu Dantani Abdullahi, Kridsada Unban, Chalermpong Saenjum, Pratthana Kodchasee, Napapan Kangwan, Hathairat Thananchai, Kalidas Shetty, Chartchai Khanongnuch Bacterial pathogens have remained a major public health concern for several decades.

Chlorine dioxide (ClO2) gas is widely recognized as a potent antimicrobial agent effective against various microorganisms. Chia seeds are prone to microbial contamination, and thermal treatments are not feasible due to potential quality issues. The study is aimed at investigating the effectiveness of chlorine dioxide (ClO2) gas followed by mild heat for Salmonella reduction in chia seeds.

The activation of the immune system reduces the nutrient availability for growth and increases the variability among pigs. Functional amino acids (AA) supplementation can reduce the negative effects of immune activation. Additionally, providing daily tailored diets using individual precision feeding (IPF) was shown to account for individual variability.

Abstract Aims Our aim is to characterize through whole‐genome sequencing (WGS) the antimicrobial resistance (AMR) and heavy metal tolerance (HMT) genes content, plasmid presence, virulence potential and genomic diversity of the rare non‐typhoid Salmonella enterica serovar Orion (S. Orion) from 19 countries of the African, American, Eastern Mediterranean, European, Southeastern Asia and Western Pacific regions. Methods and Results Totally 324 S.

The study compared the effects of the flow regimes (laminar, transitional, and turbulent), treatment time, and the volume of water and plasma‐activated water bubbles circulation on the inactivation of the mixed‐species biofilms of Salmonella Typhimurium and Aeromonas australiensis grown on the inner surfaces of the polyvinyl chloride pipes. Abstract Biofilm formation in broiler drinking water systems is a public health concern.

Microbiology Resource Announcements, Ahead of Print. A Salmonella isolate from retail pork was whole genome sequenced using Illumina NovaSeq6000, with a 5,320,119 bp genome and 51.06% GC content. Several antibiotic resistance genes and plasmids, including blaTEM-1, aac(6')-IIc, IncHI2, and p0111 were obtained from subsequent analysis.

Microbiology Resource Announcements, Ahead of Print. Whole-genome sequencing (WGS) was used to characterize four Salmonella enterica Enteritidis isolates from poultry (n=2) and human (n=2) from Ouagadougou, Burkina Faso. Antimicrobial resistance genes, chromosomal mutations, and mobile genetic elements were identified by analysis of WGS data using sequence homology.
 

Validation of traditional crust pepperoni pizza baking process. Abstract The objective of this study was to validate a simulated commercial baking process for traditional crust pepperoni pizza to inactivate Salmonella when contamination was introduced through inoculated flour and pepperoni slices.

Background

The pet industry is expanding worldwide, particularly raw meat-based diets (RMBDs). There are concerns regarding the safety of RMBDs, especially their potential to spread clinically relevant antibiotic-resistant bacteria or zoonotic pathogens.

Aim

Background For pathogen detection in food, there are occasions where samples cannot be processed immediately after selective enrichment or need to be reexamined days or weeks later for confirmation or retest. Objective This study aimed to investigate the effect of different prolonged period of storage of selective enrichments of food at 4 ± 2°C and room temperature (20–22°C) on the detection and isolation of Salmonella.

This study investigates if a mixture of ribwort, sorrel, and yarrow extracts can reduce the thermal resistance of Salmonella Enteritidis in ground chicken. The thermal death kinetics calculated by curve fitting to the log‐linear and Weibull models revealed that the herbal extract mixture may provide Salmonella‐free cooked chicken products.