Research Publications (Food Safety)

Author(s): Taekeon Jung, Yugyung Jung, Jaehyeon Ahn, Sung Yang

Author(s): S. Kumar, M. Singh, D.E. Cosby, N.A. Cox, H. Thippareddi

Author(s): Pratima Adhikari, Sudhir Yadav, Douglas E. Cosby, Nelson A. Cox, Joshua A. Jendza, Woo Kyun Kim

Salmonella enterica subsp. enterica serovar Enteritidis has been the prevalent serovar isolated from gastroenteritis cases in Brazil since the 1990s. Here, we report the draft genomes of 30 S. Enteritidis isolates originating from a variety of patients and implicated foods during outbreaks between 1999 and 2006 in Brazil.

Chagas disease, caused by the hemoflagellate protist Trypanosoma cruzi, affects nearly 6 million people worldwide, mainly in Latin America. Hematophagous triatomine insects (“kissing bugs”) are the primary vectors of T. cruzi throughout the Americas and feed on a variety of animals, including humans. Control of triatomines is central to the control of T. cruzi infection.

The enteric pathogen Salmonella enterica can interact with parts of the plant immune system despite not being a phytopathogen. Previous transcriptomic profiling of S. enterica associating with tomato suggested that Salmonella was responding to oxidative and nitrosative stress in the plant niche.

by Lene Bungenstock, Amir Abdulmawjood, Felix Reich

Author(s): Beibei Zhang, Guang Li, Muhammad Suhaib Shahid, Liping Gan, Hao Fan, Zengpeng Lv, Shaojia Yan, Yuming Guo

Author(s): G.K. Walker, M.M. Suyemoto, L.B. Borst, J. Brake

ABSTRACT

In this study, a novel dual priming oligonucleotide (DPO) system‐based polymerase chain reaction (PCR; DPO system‐based PCR) assay, which detected the fimY gene of Salmonella, was developed for the fast food testing. The DPO system‐based PCR assay allowed a wide range of annealing temperatures at 48–68°C to efficiently amplify fimY gene with an analytical detection limit of 1.2 × 102 CFU/ml for Salmonella in pure cultures and artificially contaminated food matrix.

Reactive oxygen species (ROS) are small oxygen-derived molecules that are used to control infections by phagocytic cells. In macrophages, the oxidative burst produced by the NOX2 NADPH-oxidase is essential to eradicate engulfed pathogens by both oxidative and non-oxidative killing.

M. Jiang et al. In July 2018, an outbreak of 10 cases of Salmonella enterica serovar Enteritidis infection occurred in Shenzhen, China. Outbreak investigation complemented by whole-genome sequencing traced the source to food ordered online. Our investigation highlights the role of online food delivery platforms as a new mode of foodborne disease transmission.

Bacterial resistance to antibiotics is a major concern worldwide, leading to an extensive search for alternative drugs. Promising candidates are antimicrobial peptides (AMPs), innate immunity molecules, shown to be highly efficient against multidrug resistant bacteria. Therefore, it is essential to study bacterial resistance mechanisms against them. For that purpose, we used experimental evolution, and isolated a Salmonella enterica serovar typhimurium-resistant line to the AMP 4DK5L7.

Dairy cows are known reservoirs of Salmonella enterica and human salmonellosis has been attributed to the consumption of contaminated dairy and beef products as well as poultry meat and eggs. Although many S. enterica serovars are known to colonize the gastrointestinal tract of cattle, the interactions between dairy commensal (or persistent) and transient Salmonella serovars with bovine epithelial cells are not well understood.

Author(s): G. Bhuvana Priya, Ravi Kant Agrawal, A. Arun Prince Milton, Madhu Mishra, S.K. Mendiratta, Ashish Luke, Sofia Inbaraj, Bhoj Raj Singh, Deepak Kumar, Ravi Kumar GVPPS, Swaraj Rajkhowa

In this study, a real-time loop-mediated isothermal amplification (Rti-LAMP) combined with immunomagnetic beads separation (IMS) assay and SDS rinse was developed for the rapid and sensitive detection of Salmonella in ready-to-eat (RTE) meat without prior enrichment. Polyclonal antibody and specific immunomagnetic beads (IMBs) for Salmonella flagellum were prepared.

ABSTRACT Current study aims to characterize the influence of sub-minimum inhibitory concentration (sub-MIC) of ciprofloxacin on Salmonella intracellular survival and host virulence. Herein, Salmonella resistance patterns to various antibiotics were in agreement with those reported in previous studies.

ABSTRACT Nondairy fermented products, such as fruit and vegetable juices, are gaining popularity among consumers worldwide for health‐related and economic reasons. The purpose of this study was to determine the changes in microbiological quality; antimicrobial and antioxidant activity; and phenolic, vitamin C, minerals, and cadmium content occurring during spontaneous fermentation of curly kale juice.

We previously described how four deleterious synonymous mutations in the Salmonella enterica rpsT gene (encoding ribosomal protein S20) result in low S20 levels that can be compensated by mutations that restore [S20]. Here, we have further studied the cause for the deleterious effects of S20 deficiency and found that the S20 mutants were also deficient in four other 30S proteins (S1, S2, S12, and S21), which is likely due to an assembly defect of the S20 deficient 30S subunits.

ABSTRACT

Author(s): Gabriella Mendes-Oliveira, Tony Z. Jin, Osvaldo H. Campanella

This study was designed to investigate the prevalence and characteristics of Salmonella in three Chinese pig abattoirs (A, B, and C) in Wuhan city in 2016. Four types of pig samples were collected and cultured for Salmonella. Salmonella was detected from 329 samples among the 1440 tested (22.9%). There was no significant difference in the overall prevalence between the first visit and the second visit and among the three abattoirs.

Author(s): Amal Senevirathne, Chamith Hewawaduge, John Hwa Lee

Worldwide emergence of Salmonella enterica serovar Newport (S. Newport) infection in humans, in parallel with a significant increasing prevalence of antimicrobial resistance (AR), is a serious public health concern. However, the prevalence of S. Newport resistance in China remains largely unknown. A retrospective study of 287 S. Newport clinical isolates collected during 1997–2018 was undertaken for characterization of AR profiles using the micro-dilution assay.