Research Publications (Food Safety)

Author(s): Samir Subedi, Lihui Du, Amritha Prasad, Barun Yadav, M.S. Roopesh

Intensive poultry production due to public demand raises the risk of contamination, creating potential foodborne hazards to consumers. The prevalence and microbial load of the pathogens Campylobacter, Salmonella, Staphylococcus aureus, and Escherichia coli was determined by standard methods at the farm level.

Chicken breast meat is considered as the main source of Salmonella infection in humans. The aim of this study was to isolate lytic bacteriophages specific for Salmonella enterica serovars Enteritidis and examine their efficacy in a cocktail for the biocontrol of Salmonella spp. in raw chicken breast meat. Four lytic phages belonging to the Myoviridae and Siphoviridae families were isolated from a river proximate to a duck farm. They exhibited broad lytic activities against 11 strains of S.

Aims Salmonella cells desiccated in an environment with low‐water activity (aw) show longer survival times and enhanced resistance to heat. However, little is known about the cellular ultrastructure of Salmonella in low‐aw environment in relation to the survival and persistence during desiccation.

Author(s): B. Tenderis, B. Kılıç, H. Yalçın, A. Şimşek

Author(s): Nasim Hatamzade Isfahani, Shaban Rahimi, Mohammad Javad Rasaee, Mohammad Amir Karimi Torshizi, Taghi Zahraei Salehi, Jesse L. Grimes

Author(s): Shaban Rahimi, Sophia Kathariou, Oscar Fletcher, Jesse L. Grimes

Author(s): Graham A.J. Redweik, Zachary R. Stromberg, Angelica Van Goor, Melha Mellata

Author(s): Muhammad Saeed, Muhammad Naveed, Jakob Leskovec, Asgahr Ali kamboh, Ihsanullah Kakar, Kifayat Ullah, Fawwad Ahmad, Muhammad Sharif, Asif Javaid, Mubasher Rauf, Mohamed E. Abd El-Hack, Mervat A. Abdel-Latif, Sun Chao

Author(s): Michał Patrzałek, Maja Kosecka-Strojek, Klaudia Lisowska-Łysiak, Magdalena Trela, Mariusz Kot, Magdalena Gawlak, Dawid Liszka, Mieczysław Sajewicz, Barbara Tombarkiewicz, Krzysztof Pawlak, Jacek Międzobrodzki, Marcin W. Lis

Author(s): Yuqiao Jin, Juming Tang, Mei-Jun Zhu

Author(s): Shengqi Rao, Guangwei Xu, Xiangning Lu, Ruyi Zhang, Lu Gao, Qingyan Wang, Zhenquan Yang, Xinan Jiao

ABSTRACT

Probiotics are important bacteria due to their benefit on human health. In this study, four strains of lactic acid bacteria from chicken bile were isolated and the strain with the best antimicrobial activity was selected for further identification and evaluation on its probiotic traits and safety. The strain was identified as Enterococcus faecium by biochemical characterization and 16S rDNA gene sequencing. The strain, named E.

Salmonella enterica serovar Typhimurium colonizes and invades host intestinal epithelial cells using the Type Three Secretion System (T3SS) encoded on Salmonella Pathogenicity Island 1 (SPI1). The level of SPI1 T3SS gene expression is controlled by the transcriptional activator HilA, encoded on SPI1. Expression of hilA is positively regulated by three homologous transcriptional regulators, HilD, HilC and RtsA, belonging to the AraC/XylS family.

Group A streptococcus (GAS) is a versatile pathogen that causes a wide spectrum of diseases in humans. Invading host cells is a known strategy for GAS to avoid antibiotic killing and immune recognition. However, the underlying mechanisms of GAS resistance to intracellular killing need to be explored. Endothelial HMEC-1 cells were infected with GAS, methicillin-resistant Staphylococcus aureus (MRSA) and Salmonella Typhimurium under nicotinamide (NAM)-supplemented conditions.

The aim of the present study was to estimate the prevalence of Salmonella and investigate the dominant serovars distribution in raw beef and to screen the isolated serovars for the prescense of beta-lactamases and virulence genes. A total of 150 samples of raw beef sold at butcher shops (n = 75) and supermarkets (n = 75) in Karachi city were collected (50 samples each from muscles, lymph nodes, and minced beef). The samples were cultured according to the ISO-6579-1guidlines.

Author(s): Corrella S Detweiler

Author(s): Silvia Guillén, María Marcén, Pilar Mañas, Guillermo Cebrián

Author(s): Siyuan Zhou, Tony Jin, Shiowshuh Sheen, Guohua Zhao, LinShu Liu, Vijay Juneja, Kit Yam

Author(s): Feng Xu, Chongtao Ge, Hao Luo, Shaoting Li, Martin Wiedmann, Xiangyu Deng, Guangtao Zhang, Abigail Stevenson, Robert C.s Baker, Silin Tang

Author(s): Danielle M. Schultze, Ricardo Couto, Feral Temelli, Lynn M. McMullen, Michael Gänzle

Aims The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy post‐weaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). Methods and Results Out of the 128 isolates, L. plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. L.

Here, we present the annotated genome of Shemara, a siphophage of Salmonella enterica. The Shemara genome is 44 kb with 83 predicted protein-coding genes. At the nucleotide and amino acid levels, Shemara is most similar to phages in the Guernseyvirinae subfamily.