Research Publications (Food Safety)

Salmonella enterica is a major global foodborne pathogen that causes gastroenteritis and, in some cases, death. Salmonella serovar Anatum has been increasingly associated with foodborne salmonellosis outbreaks. In this report, we announce two complete genome sequences of Salmonella Anatum isolated from papaya fruit.

Author(s): ZAHRA H. MOHAMMAD, ELSA A. MURANO, ROSANA G. MOREIRA, ALEJANDRO CASTILLO

High prevalence of antimicrobial resistance (AMR) to drugs of critically/highly importance of human and veterinary medicine was observed among the recovered Escherichia coli, Salmonella spp., and Staphylococcus aureus, circulating over the different stages of poultry in India. Upward trend in AMR rates was observed in poultry‐retail shops than in poultry‐farms. Multi‐drug resistance was observed in >90% of all the isolates.

by Catalina Pardo-Roa, Geraldyne A. Salazar, Loreani Noguera, Francisco J. Salazar-Echegarai, Omar P. Vallejos, Isidora Suazo, Bárbara M. Schultz, Irenice Coronado-Arrazola, Alexis M. Kalergis, Susan M. Bueno

Author(s): Elvira Frías, Yenea Iglesias, Avelino Alvarez-Ordóñez, Miguel Prieto, Montserrat González-Raurich, Mercedes López

Author(s): Haijing Hu, Kun Jia, Huhu Wang, Xinglian Xu, Guanghong Zhou, Shuwen He

Author(s): Jin-Young Han, Won-Jae Song, Joo Hyun Kang, Sea C. Min, Sangheum Eom, Eun Jeong Hong, Seungmin Ryu, Seongbong Kim, Sangwoo Cho, Dong-Hyun Kang

Fluoroquinolones are used to treat serious bacterial infections, including those caused by Escherichia coli and Salmonella enterica. The emergence of plasmid-mediated quinolone resistance (PMQR) represent a new challenge to the successful treatment of Gram-negative infections. As part of a long-term strategy to generate a reference database of closed plasmids from antimicrobial resistant foodborne bacteria, we performed long-read sequencing of 11 E.

Salmonella Typhimurium, a bacterial pathogen with high metabolic plasticity, can adapt to different environmental conditions; these traits enhance its virulence by enabling bacterial survival. Neutrophils play important roles in the innate immune response, including the production of microbicidal reactive oxygen species (ROS).

Author(s): Tareq M. Osaili, Fayeza Hasan, Dinesh Kumar Dhanasekaran, Reyad S. Obaid, Anas A. Al-Nabulsi, Sowmya Rao, Hera Fatima, Mutamed Ayyash, Ioannis Savvaidis, Richard Holley

Bacterial lipopolysaccharides are major components and contributors to the integrity of Gram-negative outer membranes. The more conserved lipid A-core part of this complex glycolipid is synthesized separately from the hypervariable O-antigenic polysaccharide (OPS) part and they are joined in the periplasm prior to translocation to the outer membrane.

Bactericidal effects of various concentrations of phenyllactic acid on Shiga toxin–producing Escherichia coli (STEC), including E. coli O157:H7, O26:H11, O103:H2, and O121:H19, and on Salmonella Typhimurium DT104 in pure culture and microplates assays were studied. Beef cuts were surface sprayed with phenyllactic acid or lactic acid for inactivation of E. coli O157:H7 and Salmonella Typhimurium. The 1.5% phenyllactic acid inactivated all inoculated E.

Author(s): Chang-Wei Lei, Yu Zhang, Zhuang-Zhuang Kang, Ling-Han Kong, Yi-Zhi Tang, An-Yun Zhang, Xin Yang, Hong-Ning Wang

Author(s): Mariana Cap, Paola Fleitas Paredes, Diego Fernández, Marina Mozgovoj, Sergio R. Vaudagna, Anabel Rodriguez

Author(s): Stéfani T.A. Dantas, Carlos H. Camargo, Monique R. Tiba-Casas, Ricardo C. Vivian, José P.A.N. Pinto, José C.F. Pantoja, Rodrigo T. Hernandes, Ary Fernandes Júnior, Vera L.M. Rall

Author(s): Brian Friker, Marina Morach, Sabrina Püntener, Nicole Cernela, Jule Horlbog, Roger Stephan

Abstract

In recent years, popularity of raw milk has increased in many industrialised countries.

Stenotrophomonas maltophilia is an emerging opportunistic pathogen that, on the one hand, causes severe nosocomial infection in immunocompromised populations with a high mortality rate and, on the other hand, is present ubiquitously in the environment. This study, for the first time to the best of our knowledge, isolated and characterized S. maltophilia from leafy green vegetables produced by hydroponic farms and from a hydroponic farming facility in Singapore. Eleven S.

Salmonella is one of the most common food-borne pathogens. It can be transmitted between chickens, as well as to people by contaminated poultry products. In our study, we distinguished chickens with different resistances mainly based on bacterial loads. We compared the cecal tonsil transcriptomes between the susceptible and resistant chickens after Salmonella infection, aiming to identify the crucial genes participating in the antibacterial activity in the cecal tonsil.

In The Lancet Infectious Diseases, the GBD 2017 Non-Typhoidal Salmonella Invasive Disease Collaborators1 estimate the global burden of invasive non-typhoidal salmonella disease using Bayesian meta-regression tools. The results show a high but decreasing number of cases since 2005 with a continuously high case fatality rate, consistent with previous estimates.

Single-nucleotide polymorphisms (SNPs) are widely used for whole-genome sequencing (WGS)-based subtyping of foodborne pathogens in outbreak and source tracking investigations. Mobile genetic elements (MGEs) are commonly present in bacterial genomes and may affect SNP subtyping results if their evolutionary history and dynamics differ from that of the bacterial chromosomes.

Author(s): Clarissa Silveira Luiz Vaz, Daiane Voss-Rech, Luana Alves, Arlei Coldebella, Liana Brentano, Iara Maria Trevisol

Author(s): Mathieu Gand, Wesley Mattheus, Nancy H.C. Roosens, Katelijne Dierick, Kathleen Marchal, Sigrid C.J. De Keersmaecker, Sophie Bertrand