Research Publications (Food Safety)

Salmonella biofilms act as a continuous source for cross-contamination in the food processing environments. In this study, a stable rugose morphotype of Salmonella was first induced by sequential exposure to subinhibitory concentrations (SICs) of sodium hypochlorite (NaOCl) (ranging from 50 to 300 ppm over 18-day period) in tryptic soy broth.

The enteric pathogen, Salmonella enterica is a major cause of human gastroenteritis globally and with increasing bacterial resistance to antibiotics, alternative solutions are urgently needed. Single domain antibodies (sdAbs), the smallest antibody fragments that retain antigen binding specificity and affinity, are derived from variable heavy-chain only fragments (VHH) of camelid heavy-chain-only immunoglobulins.

Author(s): Min-Jin Jeon, Jae-Won Ha

Author(s): Yuanyuan Song, Xuetong Fan

Our previous study showed that the srfABC operon, which was originally identified in Salmonella enterica as an SsrB-regulated operon clustered with the flagellar class 2 operon, exhibited significant cytotoxicity against insect midgut CF-203 cells and injectable insecticidal activity against Helicoverpa armigera larvae. The srfABC operon was widely distributed among bacteria, which raises the question of their biological roles in different species.

Author(s): Christina Sakarikou, Dimitra Kostoglou, Manuel Simões, Efstathios Giaouris

Author(s): Diana Alves, Miguel A. Cerqueira, Lorenzo M. Pastrana, Sanna Sillankorva

Author(s): Andrea R. McWhorter, Kapil K. Chousalkar

Growth behavior of Salmonella (A–E) and Shigella (F–J) in tomato–cucumber, modified tomato–cucumber, tahini tomato–cucumber, coleslaw, and toum sauce salads, respectively, stored for 5 days at 4, 10, and 24°C. Abstract This study evaluated the behavior of Salmonella and Shigella (5–6 log CFU/g) in tomato–cucumber (TC) salad without additives (control), TC with 1.0% lemon juice and 0.5% salt, TC with 10% wt/wt tahini, coleslaw, and toum sauce at 4, 10, or 24°C for 5 days.

In the United States, the consumption of fresh fruits and vegetables has increased during recent years as consumers seek to make healthier lifestyle choices. However, the number of outbreaks associated with fresh produce that involve cases in more than one state (multistate) has increased concomitantly.

Salmonella enterica is a natural bacterial pathogen of humans and animals that causes systemic infection or gastroenteritis. During systemic infection, Salmonella generally resides within professional phagocytes, typically macrophages, whereas gastroenteritis is caused by infection of epithelial cells. We are only beginning to understand which host pathways contribute to Salmonella survival in particular cell types.

Author(s): Paula Vasconcelos Costa, Luiza Vasconcellos, Ingrid Camelo da Silva, Valéria de Mello Medeiros, Stephen James Forsythe, Marcelo Luiz Lima Brandão

Author(s): Ling Jin, Ting Li, Bin Wu, Tan Yang, Dengchao Zou, Xuehua Liang, Liwen Hu, Ganhui Huang, Jinsheng Zhang

DNA phosphorothioation is widely used in antisense drugs. DNA PT modification also exists in bacteria. Using a purified enzyme complex, we show here for the first time how the inert DNA backbone is activated for the exchanging of oxygen with sulfur. Abstract Phosphorothioation (PT) involves the replacement of a nonbridging phosphate oxygen on the DNA backbone with sulfur. In bacteria, the procedure is both sequence‐ and stereo‐specific.

ABSTRACT We evaluated the combination of immunomagnetic separation (IMS), multiple displacement amplification (MDA), and real-time PCR to detect Salmonella from poultry environmental samples. The limits of detection (LODs) of IMS-MDA real-time PCR with different culture enrichment hours (0, 4, 6, and 8 h) were determined in artificially inoculated litter samples from a specific pathogen-free (SPF) poultry farm.

Salmonella and Campylobacter are leading human foodborne pathogens commonly associated with poultry and poultry products, and several methods to control these pathogens have been applied to poultry production.

ABSTRACT The objective of this study was to assess the effect of dietary yeast products on broiler chickens challenged with salmonella lipopolysaccharide (LPS). The chicks were divided into 8 treatments with 6 replicates and 9 birds per replicate.

ABSTRACT Salmonella enteritidis (S. enteritidis) infection has been recognized as one of the most common bacterial causes of human gastroenteritis worldwide and is closely associated with eggs. Slightly acidic electrolyzed water (SAEW) is an emerging environmentally friendly technology for disinfecting eggshell surfaces to remove dirt and pathogenic microorganisms.

ABSTRACT This study was conducted to determine the effects of essential oils and organic acids (EOA) on Salmonella Enteritidis (S. Enteritidis) challenged chickens. One-day-old specific pathogen-free (SPF) chicks (250) were randomly assigned to 5 groups, with 50 birds in each group. The treatment groups were as follows: 1) basal diet, negative control group (NC); 2) basal diet + S.

ABSTRACT Pullorum disease caused by Salmonella Pullorum is one of the most important infectious diseases in the poultry industry worldwide, which leads to serious economic losses in many developing countries because of its high mortality rate in young chicks. The traditional slide agglutination test with low cost, fast reaction, and on-site detection has been widely used in the diagnosis of Pullorum disease.

ABSTRACT The present study evaluated the efficacy of ethanol treatment (0, 30, 50, or 70%) alone or in combination with ultrasound (37 kHz, 380 W) for the reduction of natural indigenous mesophilic aerobic bacteria (MAB), coliforms, and inoculated Salmonella Typhimurium on chicken skin.

ABSTRACT The efficiency of combination treatment of slightly acidic electrolyzed water (SAEW) and ultraviolet light (UV) for inactivation of Salmonella enteritidis (S. enteritidis) on the surface of plastic poultry coops and other facility surfaces was evaluated in the presence of organic matter. The bactericidal activities of SAEW, UV + SAEW, and composite phenol (CP) for inactivating S. enteritidis were also compared.