Research Publications (Food Safety)

A prolonged outbreak of Salmonella enterica serotype Enteritidis occurred in northern France between December 2014 and April 2015. Epidemiological investigations following the initial notification on 30 December 2014 of five cases of salmonellosis (two confirmed S. Enteritidis) in young children residing in the Somme department revealed that all cases frequented the same food bank A.

To improve understanding of Salmonella Typhimurium LT2 inactivation in ground poultry carcasses, a series of experiments were carried out at multiple temperatures. Subsequently, a non-linear model was developed to predict Salmonella inactivation at composting and low rendering temperatures. The Salmonella inactivation study was conducted using bench-top experiments at 38, 48, 55, 62.5, 70, and 78°C in mixed and non-mixed reactors using ground poultry carcasses as a feedstock.

Summary

Aim

“Label Rouge” broiler free-range carcasses have been monitored since 1991, and broiler flocks since 2010, for contamination by the main foodborne zoonotic bacteria.

Escherichia coli and Salmonella spp. are considered to be the major pathogens associated with human transmissible infectious diseases in the air of poultry houses. Chlorine dioxide (ClO2) is an effective biocide against a wide range of microorganisms. Accordingly, this study investigated the efficiency of gaseous ClO2 application for disinfecting broiler houses by collecting air samples before and after fumigation using a passive method.

Salmonella enterica subsp. enterica serotype Enteritidis is a major cause of human salmonellosis worldwide; however, little is known about the genetic relationships between S. Enteritidis clinical strains and S. Enteritidis strains from other sources in Chile. We compared the whole genomes of 30 S. Enteritidis strains isolated from gulls, domestic chicken eggs, and humans in Chile, to investigate their phylogenetic relationships and to establish their relatedness to international strains.

Abstract

Aims

The object of this study was to determine the impact of only modifying the processing and/or particle size of pig feed on Salmonella shedding and faecal microbiota.

Abstract

Madushini N. Dharmasena, Catherine M. Feuille, Carly Elizabeth C. Starke, Arvind A. Bhagwat, Scott Stibitz, Dennis J. Kopecko

Abstract

Abstract

Abstract

Horizontal acquisition of novel chromosomal genes is considered to be a key process in the evolution of bacterial pathogens. However, identification of gene presence or absence could be hindered by the inconsistencies in bacterial genome annotations. Here, we perform a cross-annotation of omnipresent core and mosaic accessory genes in the chromosome of Salmonella enterica serovar Typhimurium, across a total of 20 fully-assembled genomes deposited into GenBank.

Bacterial Ghost-based vaccine development has been applied to a variety of gram-negative bacteria. Developed Salmonella Enteritidis (S. Enteritidis) ghost are promising vaccine candidates because of their immunogenic and enhanced biosafety potential. In this study, we aimed to evaluate the immunostimulatory effect of a S. Enteritidis ghost vaccine on the maturation of chicken bone marrow-derived dendritic cells (chBM-DCs) in vitro. The immature chBM-DCs were stimulated with S.

Robert A. Kazmierczak, Bettina Gentry, Tyler Mumm, Heide Schatten, Abraham Eisenstark

Mako Yamamoto, Ming Zhao, Yukihiko Hiroshima, Yong Zhang, Elizabeth Shurell, Fritz C. Eilber, Michael Bouvet, Makoto Noda, Robert M. Hoffman

Abstract

Competitive exclusion (CE) cultures, given as a single dose on the day of hatch, together with good hygienic practices has been shown to be a novel approach to control Salmonella in poultry. The ability of the CE product Broilact and 2 probiotics, FloraMax-B11 and Colostrum, to prevent Salmonella colonization in newly hatched chickens was evaluated employing a slightly modified Mead-model chicken assay.

Publication date: February 2017
, Volume 72, Part A

Author(s): Zeynal Topalcengiz, Michelle D. Danyluk

Publication date: February 2017
, Volume 72, Part A

Author(s): Keting Li, Shuyao Ye, Walid Q. Alali, Yin Wang, Xin Wang, Xiaodong Xia, Baowei Yang

Summary

Bacteriophage-encoded endolysins are highly diverse enzymes that cleave the bacterial peptidoglycan layer. Current research focuses on their potential applications in medicine, in food conservation, and as biotechnological tools. Despite the wealth of applications relying on the use of endolysin, little is known about the enzymatic properties of these enzymes, especially in the case of endolysins of bacteriophages infecting Gram-negative species.

Our goal was to develop a robust tagging method that can be used to track bacterial strains in vivo. To address this challenge, we adapted two existing systems: a modular plasmid-based reporter system (pCS26) that has been used for high-throughput gene expression studies in Salmonella and Escherichia coli and Tn7 transposition.

Human infections with Salmonella enterica subspecies enterica serovar Senftenberg are often associated with exposure to poultry flocks, farm environments, or contaminated food. The recent emergence of multidrug-resistant isolates has raised public health concerns. In this study, comparative genomics and phenotypic analysis were used to characterize 14 Salmonella Senftenberg clinical isolates recovered from multiple outbreaks in Shenzhen and Shanghai, China, between 2002 and 2011.

Classification by serotyping is the essential first step in the characterization of Salmonella isolates and is important for surveillance, source tracking, and outbreak detection. To improve detection and reduce the burden of salmonellosis, several rapid and high-throughput molecular Salmonella serotyping methods have been developed.

A total of 192 Hy-Line Brown 40-week-old Salmonella-free layers were randomly assigned to 4 dietary treatments in a 5-wk experiment to test the efficacy of probiotics on egg production and quality, excreta and intestinal microbiota of laying birds challenged with Salmonella gallinarum. Dietary treatment comprised of (1) NC; basal diet, negative control, (2) PC; basal diet + oral S.