Research Publications (Food Safety)

Salmonella spp. are among the most prevalent foodborne pathogens. Rapid identification of etiologic agents during foodborne outbreaks is of great importance. In this study, we report a traceback investigation of a Salmonella outbreak in China. Metagenomic sequencing of suspected food samples was performed on MinION and MiSeq platforms. Real-time nanopore sequencing analysis identified reads belonging to the Enterobacteriaceae family.

The effect of the application of different disinfectants on the microbial load and sensory quality of fresh-cut lettuce was evaluated during washing, and after subsequent storage at 4 °C under modified atmosphere packaging (MAP).

Salmonella species are common foodborne pathogenic bacteria. At present, most detection methods for Salmonella are unsuitable for on-site applications because they require large instruments or complicated procedures. This study developed a novel method of the on-site detection for Salmonella in food by combining the CRISPR/Cas12a system with recombinant polymerase amplification (RPA).

The reduction in antibiotic growth promoter use in poultry, due to antibiotic resistance concerns, has created a need for natural solutions that control enteric pathogens like Salmonella.

With the development of multidrug resistance in Salmonella spp. in recent years, ciprofloxacin, ceftriaxone, and azithromycin have become the principal antimicrobial agents used for the treatment of Salmonella infections. The underlying mechanisms of plasmid-mediated ciprofloxacin and ceftriaxone resistance have attracted extensive research interest, but not much is focused on azithromycin resistance in Salmonella.

The balance of microbial species in the intestine must be maintained to prevent inflammation and disease. Healthy bacteria suppress infection by pathogens and prevent disorders such as inflammatory bowel diseases (IBDs). The role of mucus in the relation between pathogens and the intestinal microbiota is poorly understood.

Poultry provides an important protein source consumed globally by human population, and simultaneously, acts as a substantial reservoir of antibiotic resistant bacterial species such as Escherichia coli, Salmonella, Campylobacter, Clostridium perfringens. These bacterial species can include commensal strains with beneficial roles on poultry health and productivity, and pathogenic strains not only to poultry but zoonotically to man.

The aim of this study was to evaluate phenotypic and genotypic AMR characteristics of Salmonella enterica isolates from Australian cattle collected through a structured national survey utilizing 1001 faecal samples collected from healthy cattle at slaughter.

Transboundary and Emerging Diseases, Accepted Article. The primary goals of this cross-sectional study were to screen various food/water, and human samples for the presence of Salmonella species, and to assess the phenotypic and genetic relationship between resistances found in food and human Salmonella isolates to critically important antibiotics.

Journal of Food Safety, EarlyView. Although the intestinal lumen is a significant way to enter pathogens such as Salmonella, they face a dense microbiota in the gut lumen. This community confers health benefits. Probiotics enhance the colonization resistance and direct inhibition of pathogens, which are essential in reducing the incidence and duration of gastroenteritis and histopathological complications of enteric pathogens.

Letters in Applied Microbiology, Accepted Article. This study was conducted to evaluate the performance of a screening protocol to detect and isolate mcr-positive E. coli and Salmonella spp. from animal caecal content and meat samples. We used a multicentre approach involving twelve laboratories from nine European countries.

Many studies have shown the efficacy of phage therapy in reducing intestinal pathogens. However, phage-based probiotic treatment is poorly studied in view of effects on the gut microbiota and intestinal inflammation. In this study, a lytic or a temperate phage (each at 4×108 PFU per day) or a streptomycin solution (40 mg per day) were administered to mice via drinking water for 31 days.

Ragi porridge, commonly consumed in South India is made from finger millet and noiyee (broken rice), and it is one of the excellent sources for probiotic bacteria. In vitro assays provided the proof that the probiotic strains isolated from ragi porridge can survive during the intestinal passage.

Raw milk is a significant vehicle for the transmission of different infections. In the present study, we focused on Salmonella enterica from raw milk and its resistance to various antibacterial drugs. Furthermore, we have investigated the antimicrobial and antibiofilm effects of essential oil (EO) obtained from Salvia officinalis L. leaves that were collected from the Aljouf region, Saudi Arabia, against S. enterica. One-dozen strains of S.

by Helit Cohen, Claire Hoede, Felix Scharte, Charles Coluzzi, Emiliano Cohen, Inna Shomer, Ludovic Mallet, Sébastien Holbert, Remy Felix Serre, Thomas Schiex, Isabelle Virlogeux-Payant, Guntram A.

The present work describes the genome sequencing and characterization of a novel Lactiplantibacillus plantarum strain assigned UTNGt21A isolated from wild Solanum quitoense (L.) fruits. In silico analysis has led to identifying a wide range of biosynthetic gene clusters (BGCs) and metabolic compounds.

Journal of the Science of Food and Agriculture, Accepted Article. Background The purpose of the present study was to examine the inactivation of Salmonella enterica (50 μL; 109 CFU/g), Listeria monocytogenes (50 μL; 109 CFU/g), and murine norovirus (MNV-1; 50 μL; 107 TCID50/mL) on whole and fresh-cut strawberries after 2-min disinfection treatments (water (H2O), chlorine 200 mg/L (NaClO), water-assisted UV-C (WUV), and th

Background Dietary supplementation with a fucoidan-rich Ascophyllum nodosum extract (ANE), possessing an in vitro anti-Salmonella Typhimurium activity could be a promising on-farm strategy to control Salmonella infection in pigs.

Sprout consumption has become more popular due to a new variety of sprouts being introduced to the market. However, sprout seeds are a major source of sprout contamination and have been linked to most sprout-associated foodborne outbreaks.

Journal of Food Safety, EarlyView. Engineered nanoparticles have been explored for their potential to serve as additives to foods, their packages, and contact surfaces because they are stable in a wide range of harsh environments and display inherent antimicrobial properties.

Many outbreaks involving Salmonella enterica in dehydrated coconut have been reported. Little is known about the survival of S. enterica in dehydrated coconut flakes at common retail or domestic storage conditions. This study evaluated the behavior of a S. enterica cocktail (S. Enteritidis PT4, S. Typhimurium PT4, S. Bredeney, S.

We developed a short-time treatment to inactivate Xanthomonas campestris and Salmonella enterica on cabbage, chili pepper, and radish seeds by a combination of chlorine dioxide (ClO2) gas and heat. ClO2 gas was evaporated from a mixed solution which contained hydrochloric acid (1 N) and sodium chlorite (100,000 ppm) (ClO2 solution).

The effects of sumac, cumin, black pepper and red pepper diethyl ether extracts on the growth of eight foodborne pathogens (FBP) and their biogenic amine (BA) production were investigated in histidine decarboxylase broth. The antimicrobial effect was determined by the minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations. Sumac extract showed the highest antimicrobial activity against FBP.

The objective of this study was to clarify the antibacterial activity and mechanism of Chrysanthemum buds' crude extract (CBCE) against Salmonella Typhimurium, and explore the potential application in cooked chicken.

Kefir grains consist of complex symbiotic mixtures of bacteria and yeasts, and are reported to impart numerous health-boosting properties to milk and water kefir beverages. The objective of this work was to investigate the microbial communities in kefir grains, and explore the possibility of deriving useful probiotic strains from them.