Research Publications (Food Safety)

Journal of Food Safety, EarlyView. Occurrences and contamination of Salmonella enterica on produce are a significant food safety issue. The objective of this research was to evaluate the effects of low-dose gamma radiation on survival and reductions of S. enterica on postharvest carrot and tomato. Irradiation treatments of 0–1 kGy were applied on produce, following pathogen inoculations in replicated experiments.

Journal of Food Safety, Volume 41, Issue 6, December 2021. Salmonella detection and isolation rely on different selective enrichment media, which can influence which serovars are detected. The objective of this study was to compare Salmonella recovery from broiler carcass rinses using three different selective enrichment protocols and three differential plating agars. Eight prechill broiler carcasses were collected at a commercial slaughter facility.

Journal of Food Safety, Volume 41, Issue 6, December 2021. Culture-based Salmonella isolation consists of nonselective pre-enrichment, followed by selective enrichment in Rappaport–Vassiliadis (RV) or tetrathionate (TT) broths, and subsequent plating on selective indicator agar. This study aimed to assess the recovery of two strains belonging to serovars Montevideo (strain ATCC-8387) and Typhimurium (strain ATCC-14028) when grown together in different media.

Journal of Food Safety, EarlyView. Salmonella spp. and Escherichia coli are well tolerant of freezing.

Journal of Food Processing and Preservation, Volume 46, Issue 1, January 2022. Salmonella enterica serotype Enteritidis (SE) is a pathogenic bacteria frequently isolated from shell eggs. Among the surface pasteurization methods, infrared heating provides an easy and eco-friendly application. To eliminate SE on the surface of shell eggs, pasteurization was applied via two different approaches.

Letters in Applied Microbiology, Volume 74, Issue 1, Page 53-62, January 2022. The present study was conducted to detect the occurrence of beta-lactamase and biofilm-producing Escherchia coli and Salmonella in apparently healthy broiler birds reared in household and contract poultry farms.

Environmental Microbiology, EarlyView. Antibiotics and heavy metals are used to treat and prevent bacterial infections in humans and food-producing animals (Argudín et al., 2019; WHO, 2021).

Molecular Microbiology, Volume 116, Issue 6, Page 1464-1475, December 2021. Invasion of the intestinal epithelium is an essential but energetically expensive survival strategy and is, therefore, tightly regulated by using specific cues from the environment. The enteric pathogen Salmonella controls its invasion machinery through the elegant coordination of three AraC-type transcription activators, HilD, HilC, and RtsA.

Molecular Microbiology, Volume 116, Issue 6, Page 1533-1551, December 2021. The SGI1-family elements that are specifically mobilized by the IncA- and IncC-family plasmids are important vehicles of antibiotic resistance among enteric bacteria. Although SGI1 exploits many plasmid-derived conjugation and regulatory functions, the basic mobilization module of the island is unrelated to that of IncC plasmids.

Molecular Microbiology, EarlyView. Anti-bacterial autophagy, known as xenophagy, is a host innate immune response that targets invading pathogens for degradation. Some intracellular bacteria, such as the enteric pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), utilize effector proteins to interfere with autophagy.

Molecular Microbiology, EarlyView. Salmonella is a major foodborne pathogen and is responsible for a range of diseases. Not all Salmonella contributes to severe health outcomes as there is a large degree of genetic heterogeneity among the 2,600 serovars within the genus. This variability across Salmonella serovars is linked to numerous genetic elements that dictate virulence.

Journal of Applied Microbiology, EarlyView. Aims Salmonella is extremely diverse, with >2500 serovars that are genetically and phenotypically diverse.

Journal of Applied Microbiology, EarlyView. Aims This study examined the effects of water activity (aw), ammonia and Corynebacterium urealyticum on the survival of Salmonella Typhimurium in sterile poultry litter. Methods and Results Sterile poultry litter inoculated with S.

Journal of Applied Microbiology, Volume 132, Issue 1, Page 279-289, January 2022. Aims Molecular characterization of extended-spectrum β-lactamases (ESBLs) among Salmonella Kentucky and Typhimurium isolates: partial sequence analysis of the types of β-lactamases found in these isolates, clonality, resistance and supposed emergence of ESBL-producing strains. Methods and Results A retrospective study surveyed the ESBLs occurring in a total of 1404 

Journal of Applied Microbiology, Volume 132, Issue 1, Page 290-297, January 2022. Aims In this study, the efficacy of using vacuumed hydrogen peroxide vapour (VHPV) to inactivate foodborne pathogens in whole dried black pepper (Piper nigrum) and powdered dried red pepper (Capsicum annuum) was evaluated. Methods and Results Black and red pepper inoculated with Escherichia coli O157:H7 and Salmonella Typhimurium were su

Journal of Applied Microbiology, EarlyView. Aims To characterize the genetic relatedness, phenotypic and genotypic antimicrobial resistance and plasmid content of 80 Salmonella Infantis strains isolated from food, humans and veterinary sources from 2013 to 2018 in Brazil. Methods and results Pulsed-field gel electrophoresis and single-nucleotide polymorphism analysis showed major clusters containing 50% and 38.8% of the strains studied respectively.

DksA is a conserved RNA polymerase-binding protein known to play a key role in the stringent response of proteobacteria species, including many gastrointestinal pathogens. Here, we used RNA-sequencing of Escherichia coli, Salmonella bongori and Salmonella enterica serovar Typhimurium, together with phenotypic comparison to study changes in the DksA regulon, during Salmonella evolution.

The objectives of this study were to determine the effect of the milling degree (MD) of Oryza sativa L. (Korean rice) on the heating rate, pathogen inactivation (Salmonella Typhimurium and Staphylococcus aureus), and color change resulting from radio-frequency (RF) heating. Rice samples inoculated with pathogens were placed in a polypropylene jar and subjected to RF heating for 0-75 s.

Salmonella contamination in a dry processing facility frequently requires removal methods that are non-aqueous. Removal of pathogens from food processing systems with a purge of uncontaminated dry food materials has been proposed, however, little is known with the respect to efficacy. In this study, survival of Salmonella on inert contact surfaces and transfer of Salmonella from inert contact surfaces to low-moisture foods was evaluated.

Salmonella enterica  has been increasingly implicated in foodborne outbreaks involving low moisture foods (LMF) during the recent decade. This study aimed to investigate the potential for persistence of  Salmonella enterica  in a range of low moisture foods (LMF) during storage at 3 temperatures.

This cross-sectional study determined the occurrence, risk factors and characteristics of Salmonella isolated from imported broiler hatching fertile eggs, hatcheries, and broiler farms in Trinidad and Tobago. Standard methods were used to isolate and characterize Salmonella isolates from two broiler hatcheries and 27 broiler farms in the country.

A series of proof of concept studies were developed to determine if a commercial bacteriophage (phage) cocktail could be utilized for the mitigation of Salmonella in bovine peripheral lymph nodes (LN). The first objective sought to determine if exogenous phage could be isolated from the LN following administration. If successful, the second objective sought to determine if once in the LN, could the phage effectively reduce Salmonella .

Salmonella is the leading cause of bacterial foodborne zoonoses in humans. Thus, the development of strategies to control bacterial pathogens in poultry is essential. Peanut skins, a considerable waste by-product of the peanut industry is discarded and of little economic value. However, peanut skins contain polyphenolic compounds identified that have antimicrobial properties.

Third-generation cephalosporins (3GCs) are preferred treatments for serious human Salmonella enterica infections. Beef cattle are suspected to contribute to human 3GC-resistant Salmonella infections. Commensal 3GC-resistant Escherichia coli are thought to act as reservoirs of 3GC resistance since they are more frequently isolated than 3GC-resistant Salmonella at beef cattle feedyards.

The objective of the present study was to analyze the prevalence of Salmonella in multiple food commodities in China by performing a meta-analysis. Accordingly, we screened studies that examined the prevalence of Salmonella in PubMed, Embase, and Web of Science databases. Methodological quality assessment and heterogeneity analyses were performed for included studies. The prevalence rate with the 95% confidence interval (95% CI) was selected as the effect size.