Research Publications (Food Safety)

While most bacterial species taken up by macrophages are degraded through processing of the bacteria-containing vacuole through the endosomal-lysosomal degradation pathway, intravacuolar pathogens have evolved to evade degradation through the endosomal-lysosomal pathway.

Salmonella enterica serovar Typhimurium (S. enterica ser. Typhimurium) is one of the main causes of human bacterial gastroenteritis. Therefore, there is an urgent need to develop a highly specific and accurate method for S. enterica ser. Typhimurium detection in food. In the study, we constructed a ratiometric electrochemical biosensor based on saltatory rolling circle amplification (SRCA) and dual-signal electrochemical readout.

Author(s): Sudarsan Mukhopadhyay, Dike O. Ukuku, Ocen M. Olanya, Kimberly Sokorai, Xuetong Fan Fresh produce remains the leading cause of foodborne illness outbreaks. Novel nonthermal technologies are needed to reduce the risk of pathogen contamination.

Author(s): Amin N. Olaimat, Murad A. Al-Holy, Mahmoud H. Abu Ghoush, Anas A. Al-Nabulsi, Tareq M. Osaili, Mutamed Ayyah, Yahya S. Al-Degs, Richard A. Holley Recently, the consumption of hummus has become popular in the United States, European countries, and Canada, and unfortunately, several foodborne outbreaks and recalls have been reported due to its contamination with Listeria monocytogenes and Salmonella enterica.

Author(s): Wangwang Shen, Hui Chen, Jiawei Geng, Ricardo A. Wu, Xiang Wang, Tian Ding The epidemiological characteristics of Salmonella spp. in pork have been widely studied in China, but the results remain inconsistent. This study aimed to summarize the epidemiological characteristics of Salmonella spp. isolated from pork, including its prevalence, serovar distribution, and antibiotic resistance rate. We systematically reviewed published studies on Salmonella spp.

Author(s): Grace Dewi, Shijinaraj Manjankattil, Claire Peichel, Shiliang Jia, Divek Nair, Zata Vickers, Timothy J. Johnson, Carol Cardona, Sally Noll, Anup Kollanoor Johny Salmonella Heidelberg (SH) is a highly invasive human pathogen for which turkeys can serve as reservoir hosts. Colonization of turkeys with SH may result in potential contamination and is a greater challenge to prevent in comminuted products.

The genus Salmonella is closely associated with foodborne outbreaks and animal diseases, and reports of antimicrobial resistance in Salmonella species are frequent.

Abstract Extending the shelf-life and ensuring microbiological safety of food products while preserving the nutritional properties are key aspects that must be addressed. Heat processing of food matrices has been the golden standard during the last decades, while certain non-thermal processing options have recently gained ground. In the present study, experimental pulsed light (PL) surface inactivation treatments of Salmonella enteritidis on almonds kernels are performed.

by Julie Haendiges, Gordon R. Davidson, James B. Pettengill, Elizabeth Reed, Padmini Ramachandran, Tyann Blessington, Jesse D. Miller, Nathan Anderson, Sam Myoda, Eric W. Brown, Jie Zheng, Rohan Tikekar, Maria Hoffmann Pistachios have been implicated in two salmonellosis outbreaks and multiple recalls in the U.S.

Author(s): Soo-Hwan Kim, Sunna Jyung, Dong-Hyun Kang We investigated the aspects of S. Typhimurium biofilms and their resistance (thermal and chemical) depending on the different morphotypes at room temperature (rdar; strain 43971 and bdar; strain 19585) and maturation temperature.

Transboundary and Emerging Diseases, Accepted Article. Poultry and poultry-derived products such as meat and eggs are among the main sources of non-typhoidal Salmonella (NTS) transmission to the human.

Enterotoxigenic Escherichia coli (ETEC) is a common enteric pathogen that causes diarrhoea in humans and animals. Lactobacillus rhamnosus LB1 (formerly named Lactobacillus zeae LB1) has been shown to reduce ETEC infection to Caenorhabditis elegans and Salmonella burden in pigs. This study was to evaluate the effect of L. rhamnosus LB1 on the gut health of lactating piglets that were challenged with ETEC.

Author(s): Wattana Pelyuntha, Kitiya Vongkamjan Salmonella contaminated in poultry meat remains an important food safety issue as this pathogen leads to a serious foodborne illness worldwide. A number of poultry meat products have often been recalled or rejected due to Salmonella contamination leading to high economic losses each year.

Our previous study revealed that Salmonella enterica serovar Schwarzengrund-contaminated areas of broiler chickens have expanded from West Japan to East Japan. The present study investigated the antimicrobial resistance and molecular characteristics of 124 S. Schwarzengrund isolates obtained from chicken meat produced in East and West Japan from 2008 to 2019.

Zoonoses and Public Health, EarlyView. Salmonella enterica subsp. enterica serovar Kentucky is frequently isolated from poultry, dairy and beef cattle, the environment and people with clinical salmonellosis globally. However, the sources of this serovar and its diversity and antimicrobial resistance capacities remain poorly described in many regions. To further understand the genetic diversity and antimicrobial sensitivity patterns among S.

Author(s): Peiyong Li, Mingyue Zhang, Guijuan Hao, Shuhong Sun Pullorum disease is characterized by white diarrhea resulting from infection by S. Pullorum, but arthritis associated with S. Pullorum infection has become increasingly frequent recently, especially in Chinese native chicken flocks. In this study, we isolated and identified 4 S. Pullorum strains from the Qingjiaoma chicken breeders with arthritis symptoms.

Author(s): Silvia Guillén, María Marcén, Ester Fau, Pilar Mañas, Guillermo Cebrián The ability of Salmonella to resist and adapt to harsh conditions is one of the major features that have made this microorganism such a relevant health hazard. However, the impact of these resistance responses on other aspects of Salmonella physiology, such as virulence and growth ability, is still not fully understood.

Foodborne pathogens can cause illnesses. Existing tools for detecting foodborne pathogens are typically time-consuming or require complex protocols. Here, we report an assay to directly analyze pathogenic genes based on CRISPR-Cas12. This new test, termed proximal DNA probe-based CRISPR-Cas12 (PPCas12), facilitates the detection of foodborne pathogens without amplification steps.

Salmonella enterica subsp. houtenae (S. houtenae) is a common subspecies in reptiles and has been implicated as a source of serious and life-threatening diseases in humans. Although occurrence and significance of S. houtenae infections have been extensively studied, the genetic features of S. houtenae have remained unknown due to a lack of available high-quality genome sequences. We obtained the complete genome sequence of S.

Journal of Food Safety, EarlyView. Electron beam technology may be integrated into the fresh produce wash water line, as an advanced oxidation process (AOP). However, prior to implementation, effectiveness of the technology must be assessed. Hydroxyl radical scavengers were used to quantify the role of yield of hydroxyl radicals in inactivation of the selected pathogen in aqueous solution.

Author(s): Xiaojuan Yang, Jiahui Huang, Qingping Wu, Jumei Zhang, Shiyuan Yang, Juan Wang, Yu Ding, Moutong Chen, Liang Xue, Shi Wu, Qihui Gu, Youxiong Zhang, Xianhu Wei Ready-to-eat (RTE) food products have been involved in numerous outbreaks of salmonellosis; however, epidemiological studies and data concerning Salmonella spp. contaminants of these foods are lacking in China.

Tetracyclines and sulfonamides are broad-spectrum antibacterial agents which have been used to treat bacterial infections for over half a century. The widespread use of tetracyclines and sulfonamides led to the emergence of resistance in a diverse group of bacteria. This resistance can be studied by searching for resistance genes present in the bacteria responsible for different resistance mechanisms.

by Runa Kuley, Kevin E. Draves, Deborah H. Fuller, Natalia V. Giltiay, Edward A. Clark, Daniela Giordano Mice lacking B cells are more susceptible to S. typhimurium infection. How B cells contribute to protective immunity against Salmonella and what signals drive their activation are still unclear. Neutrophils (Nphs), monocytes (MOs), and dendritic cells (DCs) are involved in early immune responses to control the initial replication of S.

Author(s): Shunshan Jiao, Hangjin Zhang, Meiji Liao, Zvi Hayouka, Pu Jing Many studies demonstrated that radio frequency (RF) was an effective pasteurization method for low-moisture foods (LMFs), and our previous study confirmed RF heating stress generated sublethal injured cells (SICs) of Salmonella enterica serovar Typhimurium (S. Typhimurium) in red pepper powder with initial aw≥0.53.

This cross-sectional study determined the occurrence, risk factors and characteristics of Salmonella isolated from imported broiler hatching fertile eggs, hatcheries, and broiler farms in Trinidad and Tobago. Standard methods were used to isolate and characterize Salmonella isolates from two broiler hatcheries and 27 broiler farms in the country.