Research Publications (Food Safety)

Author(s): Caili Qi, Feifei Sun, Qiling Wei, Jun Xu, Rui Li, Lin Zhang, Fang Lu, Xidi Jiang, Hengfeng Fu, Chunxiao Zhang, Lin Li The BaeSR two-component system and the AcrB efflux pump are closely associated with Salmonella resistance to antibiotics. However, the relationship between the two-component system, efflux pumps and protein phosphorylation of Salmonella is poorly understood. In this study, S.

Bacterial small RNAs (sRNAs) are important regulators of gene expression; however, the impact of natural mutations on sRNA functions has not been studied extensively.

Non-typhoidal Salmonella (NTS) infections result in self limiting gastroenteritis except in rare cases wherein manifestations of chronic infections can occur. Strategies employed by Salmonella to thrive in hostile environments of host during chronic infections are complex and multifaceted. In chronic state, a coordinated action of bacterial effectors allows reprogramming of macrophages to M2 subtype and thereby creating a permissible replicative niche.

by Newton G. Peres, Nancy Wang, Paul Whitney, Sven Engel, Meghanashree M. Shreenivas, Ian Comerford, Dianna M. Hocking, Anna B. Erazo, Irmgard Förster, Andreas Kupz, Thomas Gebhardt, Shaun R. McColl, Stephen J. McSorley, Sammy Bedoui, Richard A. Strugnell

Non-typhoidal Salmonella enterica is an important gastrointestinal pathogen causing a considerable burden of disease. Resistance to third generation cephalosporins poses a serious threat for treatment of severe infections.

Author(s): Yi-An Chen, Hsin-Yun Hsu, Hui-Erh Chai, Joseph Uknalis, Shiowshuh Sheen Impact of papaya extract (PaExt) and high pressure processing (HPP) on survival of Salmonella spp., meat quality and microstructure of chicken breast meat was studied. Samples have been pre-treated with PaExt (0.3%, w/w) in 1, 3 and 5 h, respectively, then followed by 350 and 450 MPa (10 min, 4 °C) HPP treatment.

Chicken Farmers of Canada has been progressively phasing out prophylactic use of antibiotics in broiler chicken production. Consequently, hatcheries, veterinarians, and nutritionists have been mandated to contend with less reliance on the use of preventive antibiotics. A topical concern is the increased risk of proliferation of enteric pathogens leading to poor performance, increased mortality, and compromised welfare.

Author(s): S. Yadav, P.Y. Teng, J. Choi, A.K. Singh, S. Vaddu, H. Thippareddi, W.K. Kim Poultry is the major sources of foodborne salmonellosis. Antibiotic resistance and a surge in zoonotic diseases warrant the use of natural alternatives. Glucosinolates (GLs) are naturally occurring antimicrobial compounds in rapeseed and canola.

Calf diarrhea is one of the most concerning challenges facing both the dairy and beef cattle industry. Maintaining healthy gut microbiota is essential for preventing gastrointestinal disorders. Here, we observed significantly less bacterial richness in the abnormal feces with watery or hemorrhagic morphology compared to the normal solid feces.

by Basel H. Abuaita, Anna-Lisa E. Lawrence, Ryan P. Berger, David R. Hill, Sha Huang, Veda K. Yadagiri, Brooke Bons, Courtney Fields, Christiane E. Wobus, Jason R. Spence, Vincent B. Young, Mary X. O’Riordan Salmonella enterica represents over 2500 serovars associated with a wide-ranging spectrum of disease; from self-limiting gastroenteritis to invasive infections caused by non-typhoidal serovars (NTS) and typhoidal serovars, respectively.

In Latin America, nontyphoidal Salmonella (NTS) is one of the most important etiological agents of foodborne infections; it can survive in soil, water, and food even after processing. Here, we aimed to perform a systematic review by collecting data on the prevalence, serotypes, and antimicrobial resistance (AMR) of NTS isolated from different food products in Latin America, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.

Author(s): Omar Hernández Hernández, Ana L. Gutiérrez-Escolano, Cleo Cancio-Lonches, Montserrat H. Iturriaga, Juan Ramiro Pacheco-Aguilar, Rocío Morales-Rayas, Sofía M. Arvizu-Medrano A multiplex PCR method was developed for the simultaneous detection of murine norovirus (MNV-1) as a surrogate for human norovirus (HuNoV) GI and GII, Salmonella spp., Shigella spp., and Shiga toxin producing Escherichia coli (STEC) in fresh produce.

Salmonella enterica  has been increasingly implicated in foodborne outbreaks involving low moisture foods (LMF) during the recent decade. This study aimed to investigate the potential for persistence of  Salmonella enterica  in a range of low moisture foods (LMF) during storage at 3 temperatures.

Author(s): Daniel Berdejo, Elisa Pagán, Natalia Merino, Laura Botello-Morte, Rafael Pagán, Diego García-Gonzalo The high prevalence of Salmonella enterica in milk poses a risk of considerable concern in the preservation of certain dairy products, mainly those elaborated from raw milk. Essential oils (EOs) have been proposed as a promising food preservative for such products due to their strong antimicrobial properties.

Salmonella infections in eggs with increasing morbidity and mortality exhibit worldwide prevalence. The present study intends to evaluate the efficacy of Lactobacillus reuteri Lb11 (L. reuteri Lb11, isolated from chicken intestinal tract) in inhibiting the growth of multi-drug resistant (MDR) Salmonella Enteritidis SE05 (obtained from egg content). The cell-free cell lysates (CFCL) of L.

Salmonella spp. and Escherichia coli (E. coli) are two of the deadliest foodborne pathogens in the US. Genes involved in antimicrobial resistance, virulence, and stress response, enable these pathogens to increase their pathogenicity. This study aims to examine the genes detected in both outbreak and non-outbreak Salmonella spp. and E. coli by analyzing the data from the National Centre for Biotechnology Information (NCBI) Pathogen Detection Isolates Browser database.

Salmonella spp. is one of the most common foodborne disease-causing pathogens that can cause severe diseases in very low infectious doses. Rapid and sensitive detecting Salmonella spp. is advantageous to the control of its spread. In this study, a conserved short fragment of the Salmonella invA gene was selected and used to design primers and specific crRNA (CRISPR RNA) for establishing a one-tube and two-step reaction system for Salmonella spp.

Author(s): Lili Li, Rikke Heidemann Olsen, Chong Wang, Anhua Song, Jian Xiao, Hecheng Meng, Troels Ronco, Lei Shi Salmonella enterica displaying resistance to extended-spectrum cephalosporins and fluoroquinolone (FQs) has been deemed a high-priority pathogen by the World Health Organization (WHO). While CTX-M type acquired β-lactamases have been detected in S. enterica serovar Bovismorbificans, DHA enzymes have been rarely reported in S. Bovismorbificans.

Salmonella , a major foodborne pathogen, causes severe gastrointestinal disease in people and animals worldwide. Plasmid-borne mcr-1 , which confers colistin resistance in Salmonella, has significant epidemiological interest for public health safety.

  Salmonella enterica   serovar Infantis is the fifth most common   Salmonella   serovar isolated in England and Wales.

Recycled manure solids (RMS) bedding is an alternative bedding option that is growing in popularity on Canadian dairy farms. However, the microbiological characteristics and production of RMS bedding are poorly documented under on-farm conditions in eastern Canada. This bedding could support the presence of pathogens and could have an effect on cow and human health. The aim of this study was to describe the RMS microbiota when used under dairy cows and compare it with straw bedding.

The emergence of multidrug-resistant microorganisms represents a global challenge that has led to a search for new antimicrobial compounds. Essential oils (EOs) from medicinal aromatic plants are a potential alternative for conventional antibiotics. In this study, the antimicrobial and anti-biofilm potential of 15 EOs was evaluated on planktonic and biofilm-associated cells of Salmonella enterica serovar Enteritidis ATCC 13076 (S.

Letters in Applied Microbiology, Accepted Article. The present study was conducted to detect the occurrence of beta-lactamase and biofilm-producing-E. coli and Salmonella in apparently healthy broiler birds reared in household and contract poultry farms. In total, 150 cloacal swabs were collected from apparently healthy broiler birds of various age groups reared in backyard (n=100) and contract farms (n=50) inWest Bengal (India).

The objectives of this study were to determine the effect of the milling degree (MD) of Oryza sativa L. (Korean rice) on the heating rate, pathogen inactivation (Salmonella Typhimurium and Staphylococcus aureus), and color change resulting from radio-frequency (RF) heating. Rice samples inoculated with pathogens were placed in a polypropylene jar and subjected to RF heating for 0-75 s.

Salmonella contamination in a dry processing facility frequently requires removal methods that are non-aqueous. Removal of pathogens from food processing systems with a purge of uncontaminated dry food materials has been proposed, however, little is known with the respect to efficacy. In this study, survival of Salmonella on inert contact surfaces and transfer of Salmonella from inert contact surfaces to low-moisture foods was evaluated.