Research Publications (Food Safety)

The first step in quantitative microbial risk assessment (QMRA) is to determine distribution of pathogen contamination among servings of the food at some point in the farm-to-table chain. In the present study, distribution of Salmonella contamination among servings of chicken liver for use in QMRA was determined at meal preparation.

In the present study, eight strains of Enterococcus spp. were isolated from Turkish and Iranian white cheese test pieces. Enterococcus spp. strains were identified as Enterococcus faecium (6 strains) and E. faecalis (2) with 16S rDNA sequence analysis. All Enterococcus spp.

Shigella flexneri, causative agent of bacillary dysentery (shigellosis), uses a type III secretion system (T3SS) as its primary virulence factor. The T3SS injectisome delivers effector proteins into host cells to promote entry and create an important intracellular niche. The injectisome’s cytoplasmic sorting platform (SP) is a critical assembly that contributes to substrate selection and energizing secretion.

Author(s): Nitin Dhowlaghar, Juming Tang, Mei-Jun Zhu

The outer membrane of Salmonella enterica plays an important role in combating stress encountered in the environment and hosts. The transport and insertion of lipopolysaccharides (LPS) into the outer membrane involves lipopolysaccharide transport proteins (LptA-F) and mutations in the genes encoding for these proteins are often lethal or result in the transport of atypical LPS that can alter stress tolerance in bacteria. During studies of heterogeneity in bile salts tolerance, S.

A continued rise in leafy green-linked outbreaks of disease caused by pathogenic Escherichia coli or Salmonella , particularly strains exhibiting multidrug resistance

This study aimed to investigate the chemical composition and the activity against Staphylococcus aureus (S. aureus) (ATCC 25923), Streptococcus pyogenes (S. pyogenes) (ATCC 19615), Escherichia coli (E. coli) (ATCC 25922), Pseudomonas aeruginosa (P. aeruginosa) (ATCC 27853), Shigella flexneri (S. flexneri) (ATCC 12022), Salmonella typhimurium (S. typhimurium) (ATCC 14028), Haemophillus influenzae (H. influenza) type B (ATCC 10211) and two fungal strains: Candida albicans (C.

Lacticaseibacillus rhamnosus is a recognized probiotic that is widely used in scientific research and clinical applications. This study found that the Lacticaseibacillus rhamnosus GG (LGG) strain can reduce the adhesion of Escherichia coli (E. coli) to primary chicken intestinal epithelial cells by 75.7% and inhibit 41.7% of the E. coli that adhere to intestinal epithelial cells. Additionally, LGG showed strong inhibitory ability on the growth of E.

Author(s): Shamsun Nahar, Ha Lim Jeong, Younsoo Kim, Angela Jie-won Ha, Pantu Kumar Roy, Si Hong Park, Md. Ashrafudoulla, Md. Furkanur Rahman Mizan, Sang-Do Ha

Author(s): Mahmoud F. Gawish, Ashraf M. Ahmed, Helmy A. Torky, Tadashi Shimamoto

The study aimed to examine the prevalence and genetic characteristics of ESBL-production and colistin resistance in Salmonella and Escherichia coli from pigs and pork in the border area among Thailand, Cambodia, Lao PDR, and Myanmar. Salmonella (n = 463) and E. coli (n = 767) isolates were collected from pig rectal swab from slaughterhouses (n = 441) and pork from retail markets (n = 368) during October 2017 and March 2018.

Salmonella is a major foodborne pathogen that causes foodborne disease in humans through consumption of contaminated foods, especially those of animal origin. Multiple Salmonella strains are antibiotic-resistant due to the common use of antibiotics in farm animals, including broiler farms. In this study, an alternative strategy using phage-based treatment was evaluated against Salmonella isolated from the broiler production. The prevalence of Salmonella spp.

ABSTRACT Stachys pilifera Benth is an endemic species of Stachys family found in Iran with a wide application as an herbal tea. The objective of this research was to evaluate the antimicrobial, antioxidant and cytotoxic activity of the essential oil from the aerial parts of S. pilifera.

by Deyanira Pérez-Morales, Jessica Nava-Galeana, Roberto Rosales-Reyes, Paige Teehan, Helen Yakhnin, Erika I. Melchy-Pérez, Yvonne Rosenstein, Miguel A. De la Cruz, Paul Babitzke, Víctor H. Bustamante

Author(s): Erlany Monteiro Ribeiro Pelissari, Karina Vieira Covre, Denes Kaic Alves do Rosario, Jackline Freitas Brilhante de São José

The U.S. is one of the largest catfish producers in the world. Louisiana is the leading producer of wild-caught catfish. Historically, the FDA inspected all seafood products; however, in 2008, congress moved the Siluriformes Order fish inspection to the USDA/FSIS. Full enforcement of the rule began on September 1, 2017.

Salmonella is a common cause of foodborne illness in the U.S. and often is attributed to chicken products. Previous studies have associated Salmonella contamination with meat processing facility characteristics such as the number of establishment employees (i.e., HACCP size). An evaluation of risk factors for Salmonella contamination in U.S. poultry has not been performed since implementation of the New Poultry Inspection System (NPIS) in 2014.

Journal of Food Safety, EarlyView. Salmonella spp. are one of the main causes of foodborne disease in Europe and have been associated with consumption of pig meat. However, Salmonella monitoring programmes in pigs and pig meat are not harmonized between those European countries where they exist. In general, current control programmes in Europe can be split into those aiming for: (a) elimination of infection and (b) control and reduction.

Whole-genome sequencing (WGS)-based outbreak investigation has proven to be a valuable method for the surveillance of bacterial pathogens. Its utility has been successfully demonstrated using both gene-by-gene (cgMLST or wgMLST) and single-nucleotide polymorphism (SNP)-based approaches.

To characterize the formation mechanism and characteristics of two cointegrate plasmids in Salmonella enterica serotype Enteritidis strain S13, plasmids from strain S13 and three corresponding transconjugants were subjected to whole genome sequencing and analyzed using bioinformatics tools. The traits of two fusion plasmids in transconjugants were characterized by stability and conjugation experiments.

Author(s): Damkerng Bundidamorn, Wannakarn Supawasit, Sudsai Trevanich

Salmonella is the primary cause of community-acquired foodborne infections, so its resistance to antimicrobials, such as aminoglycosides, is a public health issue. Of concern, aminoglycoside resistance in Salmonella is increasing rapidly. Here, we performed a retrospective study evaluating the prevalence of Salmonella harboring armA-mediated aminoglycoside resistance in community-acquired infections and in food or environmental sources.

Salmonella enterica subspecies enterica serovar Derby (S. Derby) is one of the most frequent causes for salmonellosis in humans and animals. Understanding the genetic diversity of S. Derby, as well as the nature and origin of its resistance to antimicrobial treatment are thus the key to epidemiological control and surveillance. Here, we report an analysis of 15 S.

Food poisoning associated with microbial pathogens causes millions of foodborne disease cases in the Kingdom of Saudi Arabia. Awareness about foodborne pathogens may play a positive role in the reduction of foodborne illnesses. The present cross-sectional study investigates awareness about five major microbial pathogens (Salmonella, Campylobacter, Staphylococcus, Listeria, and Escherichia coli) among university students () from four major cities in the Kingdom of Saudi Arabia.

Author(s): Anamaria M.P. dos Santos, Pedro Panzenhagen, Rafaela G. Ferrari, Grazielle L. Rodrigues, Carlos A. Conte-Junior