Research Publications (Food Safety)

Pseudomonas fluorescens is a specific spoilage microorganism of refrigerated marine fish, which possesses strong adaptability to low temperature. Cold shock proteins (CSPs) play an important role in bacterial cold adaptation. In this study, the CSP genes were obtained from the genome of P. fluorescens PF08 by search the conserved domain of CSPs through HMMER software, and their physicochemical property, structure and function were analyzed by bioinformatics.

Author(s): Wentao Jiang, Carly Waldman, KaWang Li, Jacek Jaczynski, Cangliang Shen

β-phenylethylamine hydrochloride (PEA-HCl) and ethyl acetoacetate (EAA) are anti-microbials with applications in food processing. As food anti-microbials, the compounds will have to withstand the cooking process without changing to toxic compounds. With this Communication, we address the question of whether PEA and EAA are altered when heated to 73.9 °C or 93.3 °C.

Antibiotic resistant bacteria and antibiotic residues can enter the environment when using animal manure as fertilizer. Twenty-five mixed beef cattle farmyard manure samples and 9 mixed fattening calf slurry samples from different farms across Belgium were investigated for the presence of 69 antibiotic residues, antibiotic resistant Escherichia coli and Salmonella spp.

Foodborne pathogens, microbial recurrent infections, and antibiotic resistance have driven researchers to explore natural compounds as safe alternative antimicrobials. In this study, the chemical profile, antimicrobial, and mutagenic activities of the Elletaria cardamomum essential oil were investigated.

The study aimed to evaluate qualitatively and quantitatively the antimicrobial capacity of 10 potential probiotic Lactobacillus strains against model enteropathogens and spoilage microorganisms. The probiotic strains (live and heat-killed forms) were also assessed for their ability to inhibit adhesion of selected pathogens to Caco-2 cells.

Food-borne outbreak investigation currently relies on the time-consuming and challenging bacterial isolation from food, to be able to link food-derived strains to more easily obtained isolates from infected people. When no food isolate can be obtained, the source of the outbreak cannot be unambiguously determined.

Salmonella enterica remains one of the leading causes of foodborne bacterial disease. Retail meat is a major source of human salmonellosis. However, comparative genomic analyses of S. enterica isolates from retail meat from different sources in China are lacking. A total of 341 S. enterica strains were isolated from retail meat in sixteen districts of Beijing, China, at three different time points (January 1st, May 1st, and October 1st) in 2017.

by Clifford G. Clark, Ashley K. Kearney, Lorelee Tschetter, James Robertson, Frank Pollari, Stephen Parker, Gitanjali Arya, Kim Ziebell, Roger Johnson, John Nash, Celine Nadon

Author(s): Yi-Zhou Xiang, Yan-Mei Zhang, Ying-Yang Liu, Man Zhang, Lian-Bing Lin, Qi-Lin Zhang

Journal of Applied Microbiology, Accepted Article. Aim
The aim of this study was to develop a saltatory rolling circle amplification (SRCA) assay for rapid, simple and visual detection of Salmonella in meat.

Author(s): Cong Shen, Furong Ma, Suiyan Deng, Lan-Lan Zhong, Mohamed Abd El-Gawad El-Sayed Ahmed, Guili Zhang, Bin Yan, Min Dai, Fan Yang, Yong Xia, Guo-Bao Tian

Background
Previous studies reported the prevalence of mcr-1 among clinical infected Salmonella isolates in China. However, the transmission dynamics of mcr-1 in different ecological niches were not well investigated. Our objective is to exhibit the transmission dynamics of mcr-1 in Salmonella.

Author(s): Lin Li, Rui Li, Caili Qi, Haixia Gao, Qiling Wei, Lei Tan, Feifei Sun

Author(s): Shang Wu, Yang Yang, Tingwei Wang, Jiadi Sun, Yinzhi Zhang, Jian Ji, Xiulan Sun

Author(s): Hong Bai, Donggen Zhou, Xiaowei Zhang, Yifang Cao, Xinglong Xiao, Yan Zhang, Yigang Yu

Hydrogen peroxide (H 2 O 2 ) has long been studied as an aqueous sanitizer to enhance microbial safety of fresh produce. Recently, we demonstrated that cold plasma-activated H 2 O 2 aerosols, hereafter referred to as ionized hydrogen peroxide (iHP), reduced populations of Salmonella, Listeria, and E. coli by up to 5.5 logs on surfaces of various produce items. However, the amount and fate of H 2 O 2 residue left on fresh produce after treatments have not been evaluated.

Here, a Bacillus strain, UTK D1-0055, is described that was isolated from a laboratory environment. It was of interest as it demonstrated antilisterial activity. The genome was sequenced, which identified the strain as Bacillus altitudinis, and a high-quality complete annotated genome was produced. Additionally, the taxonomy of this and related species was evaluated, including B. aerophilus, B. pumilus, B. safensis, B. stratosphericus, and B.

Dried parsley is regularly contaminated with foodborne pathogens, especially Salmonella (S.) spp. Application of contaminated ingredients in ready-to-eat dishes without further thermal treatment represents a considerable health risk. This study examines the suitability of pulsed light as a novel decontamination method of Salmonella spp.

Recently, ready-to-eat vegetable salads have gained popularity worldwide. However, the microbial safety of ready-to-eat salads is a health concern, primarily due to Salmonella enteritidis contamination during the growing, harvesting, processing, and handling of produce. This study aimed to develop a bacteriophage-based strategy to control S. enteritidis growth in mixed-ingredient salads.

Author(s): Xiaoming Wang, Zhuoren Ling, Naiyan Sun, Yongshi Liu, Jinhu Huang, Liping Wang

by Daniel F. M. Monte, Fábio P. Sellera, Ralf Lopes, Shivaramu Keelara, Mariza Landgraf, Shermalyn Greene, Paula J. Fedorka-Cray, Siddhartha Thakur

Zoonoses and Public Health, EarlyView. Salmonella infections are a major human health concern. In the elderly and immunocompromised, infections can be life‐threatening and may require antibiotic therapy. Where antibiotic therapy is required, antimicrobials of choice include fluoroquinolones and extended‐spectrum cephalosporins (ESC).

The CRISPR-Cas cluster is found in many prokaryotic genomes including those of the Enterobacteriaceae family. Salmonella enterica serovar Typhi (S. Typhi) harbors a Type I-E CRISPR-Cas locus composed of cas3, cse1, cse2, cas7, cas5, cas6e, cas1, cas2, and a CRISPR1 array.

Background

Author(s): Trond Møretrø, Christophe Nguyen-The, Pierrine Didier, Isabelle Maître, Tekla Izsó, Gyula Kasza, Silje E. Skuland, Maria João Cardoso, Vania B. Ferreira, Paula Teixeira, Daniela Borda, Loredana Dumitrascu, Corina Neagu, Anca Ioana Nicolau, Eduard Anfruns-Estrada, Mike Foden, Phil Voysey, Solveig Langsrud