Research Publications (Food Safety)

Zoonoses and Public Health, EarlyView. Reptiles are carriers of Salmonella and can intermittently shed bacteria in their faeces. Contact with snakes and lizards is a source of human salmonellosis. Here, two populations of reptiles, wild and captive were surveyed for Salmonella. One hundred thirty wild‐caught reptiles were sampled for Salmonella including 2 turtle, 9 snake and 31 lizard species.

Salmonella enterica serovar Enteritidis ODA 99-30581-13 is a relatively heat-resistant strain isolated from shell eggs. The strain has a 4,777,965-bp genome sequence (52.1% GC content) that was predicted to encode 4,455 proteins, including heat stress response proteins and stress response regulators; these may be involved in its heat resistance.

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by Susan M. Brewer, Christian Twittenhoff, Jens Kortmann, Sky W. Brubaker, Jared Honeycutt, Liliana Moura Massis, Trung H. M. Pham, Franz Narberhaus, Denise M. Monack

Salmonellosis is one of the most common and widely distributed food-borne diseases, and the presence of antimicrobial-resistant Salmonella in poultry and poultry products is a global public health problem.

Author(s): Cindy Shuan Ju Teh, Min Yi Lau, Chun Wie Chong, Soo Tein Ngoi, Kek Heng Chua, Way Seah Lee, Kwai Lin Thong

Author(s): Erica Tirloni, Simone Stella, Cristian Bernardi, Per Sand Rosshaug

Letters in Applied Microbiology, Accepted Article. In the present study, Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis were transferred into Luria‐Bertani medium without NaCl (LBWS) and adjusted to various pHs (4, 5, 6, and 7) with lactic acid containing 0.75%, 5%, 10%, and 30% NaCl, and stored at 25°C until the bacterial populations reached below detectable levels on tryptic soy agar (TSA). Although E. coli O157:H7 and S.

Journal of Food Processing and Preservation, Accepted Article. In this study, the effect of cholesterol on antibiotic resistance of Salmonella Typhimurium was investigated. Antibiotic resistance patterns revealed that the bacterium was susceptible to ampicillin, streptomycin, sulfamethoxazole/trimethoprim, ciprofloxacin, tetracycline, chloramphenicol, gentamicin and resistant to penicillin, erythromycin, and vancomycin.

Over the last decade, an increasing number of reports presented Galleria mellonella larvae as an important model to study host-pathogen interactions. Coherently, increasing information became available about molecular mechanisms used by this host to cope with microbial infections but few of them dealt with oxidative stress. In this work, we addressed the role of reactive oxygen species (ROS) produced by the immune system of G.

Author(s): Qixing Lin, Kapil K. Chousalkar, Andrea R. McWhorter, Samiullah Khan

Antimicrobial-resistant pathogens display significant public health threats by causing difficulties in clinical treatment of bacterial infection. Antimicrobial resistance (AMR) is transmissible between bacteria, significantly increasing the appearance of antimicrobial-resistant pathogens and aggravating the AMR problem.

Freshwater can support the survival of the enteric pathogen Salmonella, though temporal Salmonella diversity in a large watershed has not been assessed. At 28 locations within the Susquehanna River basin, 10-liter samples were assessed in spring and summer over 2 years. Salmonella prevalence was 49%, and increased river discharge was the main driver of Salmonella presence.

Inoculation studies are important when assessing microbial survival and growth in food products. These studies typically involve the pregrowth of multiple strains of a target pathogen under a single condition; this emphasizes strain diversity.

Foods of animal origin, such as poultry, eggs, and pork, are recognized sources of infection with Salmonella , but determining the proportion of foodborne infections resulting from different food sources has been challenging. In the current study, 141 Salmonella strains isolated from poultry products (n=1322) purchased over a period of one year from retail stores across Seattle, Washington, were subtyped by Pulsed Field Gel Electrophoresis (PFGE) using restriction enzyme Xba I.

The number of farmers markets registered by the U. S. Department of Agriculture (USDA) has seen a significant increase, jumping from 1,755 in 1994 to 8,771 in 2019. Microbial studies have found evidence that produce sold at farmers can yield higher microbial counts than their retail counterparts; however, no previous literature explored the efficacy of microbial (bacteria and virus) persistence on a variety of different farmers market fomites over a 2-month period.

by Iyll-Joon Doh, Huisung Kim, Jennifer Sturgis, Bartek Rajwa, J. Paul Robinson, Euiwon Bae

Introduction

Commensal and pathogenic strains of multidrug-resistant (MDR) Escherichia coli and non-typhoid strains of Salmonella represent a growing foodborne threat from foods of poultry origin. MDR strains of Salmonella Infantis and E. coli are frequently isolated from broiler chicks and the simultaneous presence of these two enteric bacterial species would potentially allow the exchange of mobile resistance determinants.

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Genes annotated as ygfE and yiiU in the genome of Salmonella enterica serovar Typhimurium encode proteins homologous to Escherichia coli cell division factors ZapA and ZapB, respectively. ZapA− and ZapB− mutants of S. enterica are bile-sensitive. The amount of zapB mRNA increases in the presence of a sublethal concentration of sodium deoxycholate (DOC) while zapA mRNA remains unaffected.

Salmonella spp. is among the main foodborne pathogens which cause serious foodborne diseases. An isothermal real-time recombinase polymerase amplification (RPA) and lateral flow strip detection (LFS RPA) were used to detect Salmonella spp. targeting the conserved sequence of invasion protein A (invA). The Real-time RPA was performed in a portable florescence scanner at 39°C for 20 min.

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Here, we report the whole-genome sequence of multidrug-resistant Salmonella enterica serovar Cannstatt harboring mcr-1.1, isolated from a fatal sepsis case. Genomic analysis revealed that the isolate was sequence type 2390 carrying mcr-1.1, blaCTX-M-14, aac(3)IId, aac(6')Iaa, floR, qnrS1, sul2, tetA, and tetM. Three Inc plasmids were observed, including the IncX4 plasmid containing mcr-1.1.

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Author(s): Xinyao Wei, Sabrina Vasquez, Harshavardhan Thippareddi, Jeyamkondan Subbiah