Research Publications (Food Safety)

ABSTRACT

Salmonella enterica serovar Livingstone 1236H was isolated originally from peanut butter and represents a health risk in low-moisture foods. The current work presents the strain’s genome sequencing results, which show a 4,824,729-bp genome sequence and 4,435 protein coding sequences, including some that are involved in adaptation to low-moisture environments.

Here, we report the complete genome sequences for 36 Canadian isolates of Salmonella enterica subsp. enterica serovar Typhimurium and its monophasic variant I 1,4,[5]:12:i:– from both clinical and animal sources. These genome sequences will provide useful references for understanding the genetic variation within this prominent serotype.

ABSTRACT

Author(s): Mario González-Angulo, Vinicio Serment-Moreno, Laura Clemente-García, Carole Tonello, Isabel Jaime, Jordi Rovira

Author(s): Xin Wang, Yiluo Cheng, Wenting Zhang, Qin Lu, Guoyuan Wen, Qingping Luo, Huabin Shao, Zishu Pan, Tengfei Zhang

Author(s): Xin Yin, Nkuchia M. M’ikanatha, Epiphanie Nyirabahizi, Patrick F. McDermott, Heather Tate

Author(s): Tania Veltman, David Jordan, Christopher A. McDevitt, Jan Bell, Benjamin P. Howden, Mary Valcanis, Mark O’Dea, Sam Abraham, Peter Scott, Jessica H. Kovac, Raymond Chia, Barry Combs, Kapil Chousalkar, Timothy Wilson, Darren J. Trott

The study evaluates the effect of Artemisia dracunculus essential oil (EO) on two pathogenic bacteria Salmonella enterica serovar Typhimurium and Staphylococcus aureus and Vero cell line. To evaluating the anti-biofilm potential of the EO, a microtiter-plate test (MtP) and scanning electron microscopy (SEM) were performed.

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ABSTRACT

The initial steps of Salmonella pathogenesis involve adhesion to and invasion into host epithelial cells. While well-studied for Salmonella enterica serovar Typhimurium, the factors contributing to this process in other, host-adapted serovars remains unexplored. Here, we screened clinical isolates of serovars Gallinarum, Dublin, Choleraesuis, Typhimurium, and Enteritidis for adhesion to and invasion into intestinal epithelial cell lines of human, porcine, and chicken origins.

In the present study, changes in the quality characteristics of the albumin and yolk parts of the eggs obtained from different poultry systems for four seasons were investigated. The highest egg weight, shell thickness, dry shell weight, shell deformation, shell fracture and vitellin membrane strength values were determined to be in cage systems with 70.92g, 0.45 mm, 9.46g, 0.79 mm, 71.80 N and 0.13 N, respectively.

This present work shows the potential of dimethyl dicarbonate (DMDC) as a promising approach to ensure quality and safety of coconut water in term of microbial inactivation and minimal changes in quality parameters during cold storage (4 °C). Pathogenic strains including Escherichia coli, Staphylococcus aureus, Salmonella Enteritidis and Listeria monocytogenes were selected for evaluation of inactivation kinetic with DMDC concentration between 0 – 300 ppm.

Gluten-free biscuits were developed with the addition of chia seeds (Salvia hispanica L.) and turmeric powder. An experimental design 22 were employed in the formulation optimization that promotes better sensory acceptance through acceptance test with a hedonic scale of 9 points. For characterization purposes, the centesimal composition from chia seeds and the turmeric powder were determined.

This work describes the characterization and genome annotation of Salmonella phage vB_SalM_8-19 (referred to as 8-19) isolated from sewage samples collected in a pig farm in Jilin, China. This phage was capable of infecting 60% Salmonella strains in our lab stock. The genome of phage 8-19 is composed of linear double-stranded DNA that is 52,648 bp in length with a G + C content of 46.02%; containing 74 ORFs and no tRNA genes.

Author(s): J. Ortiz-Solà, I. Abadias, P. Colàs-Medà, M. Anguera, I. Viñas

Author(s): Jessie Usaga, Óscar Acosta, John J. Churey, Olga I. Padilla-Zakour, Randy W. Worobo

Author(s): Yinka M. Somorin, Olumide A. Odeyemi, Collins N. Ateba

Foodborne pathogens have been associated with severe and complicated diseases. Therefore, these types of infections are a concern for public health officials and food and dairy industries. Regarding the wide-spread multidrug resistant (MDR) and extensively drug resistant (XDR) foodborne pathogens such as Salmonella Enteritidis (S. Enteritidis), new and alternative therapeutic approaches are urgently needed.

Author(s): Kye-Hwan Byun, Sang Ha Han, Jang-won Yoon, Si Hong Park, Sang-Do Ha

Avocado Hass (Persea americana Mill) peel extract (APE) has the potential as a natural ingredient to substitute for chemical preservatives.

We conducted a pilot study to assess microbiological safety of chicken litter, an affordable organic and main fertilizer used in Cameroon and worldwide. A convenience sampling of 26 farms was done and a questionnaire was administered. Samples of litter were aseptically collected. E. coli and Salmonella spp. were isolated using CLSI standards. Antibiotic susceptibility testing was performed using the disc diffusion method and a micro broth dilution method for colistin.

Author(s): Yaonan Wang, Guifeng Liu, Jian Zhang, Dan Gu, Maozhi Hu, Yunzheng Zhang, Zhiming Pan, Shizhong Geng, Xin’an Jiao