Research Publications (Food Safety)

Author(s): Gulten Izmirlioglu, Beining Ouyang, Ali Demirci

Aims Characterization of quinolone‐resistant Salmonella Kentucky and Typhimurium isolates in Tunisia from various sources, detection of some PMQR genes, and the genetic relatedness. Methods A total of 1,404 isolates of S. Kentucky (n = 1,059) / S. Typhimurium (n = 345) from various sources from all over Tunisia were tested for quinolone resistance by disk diffusion method. Minimum inhibitory concentrations of nalidixic acid, ciprofloxacin, and ofloxacin were determined.

Antimicrobial resistance (AMR) is one of the most prominent public health threats. AMR genes localized on plasmids can be easily transferred between bacterial isolates by horizontal gene transfer, thereby contributing to the spread of AMR. Next-generation sequencing (NGS) technologies are ideal for the detection of AMR genes; however, reliable reconstruction of plasmids is still a challenge due to large repetitive regions.

Isochoric freezing, different from isobaric (conventional) freezing, has a superior capability to reduce foodborne pathogens (a) photo and (b) schematic of a prototypical isochoric chamber. Abstract Isochoric freezing, different from isobaric (conventional) freezing, allows for storage below freezing temperatures without significant damage from ice formation.

ABSTRACT The emergence of plasmid-mediated colistin resistance genes (mcr), which is occurring in numerous countries, is a worldwide concern, primarily because colistin is a last-resort antibiotic. Compared to E. coli, prevalence of mcr genes in Salmonella is unclear in Japan. Here we screened for mcr-1–5 genes in our collection of Salmonella strains isolated from retail meat products collected in Japan from 2012 through 2016.

Author(s): Dayna M. Harhay, Margaret D. Weinroth, James L. Bono, Gregory P. Harhay, Joseph M. Bosilevac

Author(s): Jennifer C. Acuff, Jian Wu, Claire Marik, Kim Waterman, Daniel Gallagher, Haibo Huang, Robert C. Williams, Monica A. Ponder

Author(s): Esben Bragason, Tesfemariam Berhe, Dakalo Dashe, Kim Ib Sørensen, Mituku E. Guya, Egon Bech Hansen

Author(s): Mi-Ju Kim, Hyun-Joong Kim, Hae-Yeong Kim

Author(s): Mashael R. Aljumaah, Gamaleldein M. Suliman, Abdulaziz Alabdullatif, Alaeldein, M. Abudabos

Author(s): Dongryeoul Bae, Dong-Hyeon Kim, Jung-Whan Chon, Kwang-Young Song, Kun-Ho Seo

Salmonella enterica serovar Typhimurium is a pathogen harbored by livestock and shed in their feces, which serves as an acquisition source for adult house flies. This study used a green fluorescent protein (GFP) expressing strain of Salmonella Typhimurium to assess its acquisition by and survival within house flies, and transmission from and between flies in the presence or absence of cantaloupe.

Author(s): Ítalo Henrique Rodrigues Marques Ferreira, Geany Targino de Souza Pedrosa, Jiin Jung, Adma Nadja Ferreira de Melo, Fernanda Bovo Campagnollo, Donald W. Schaffner, Marciane Magnani

Salmonella is a leading human pathogen and a significant public health concern worldwide. Massive food production and distribution have contributed to this pathogen dissemination, which, combined with antimicrobial resistance (AMR), creates new control challenges in food safety. The development of AMR is a natural phenomenon and can occur in the bacterial evolutionary process.

Litsea cubeba (LC) is widely used as a food flavoring. In this study, 30 components of the essential oil of Litsea cubeba (LCEO) were identified, of which α‐citral (33.6534%) and β‐citral (27.8042%) were identified as the main components. The diameter of the inhibition zone, minimum inhibitory concentration, and minimum bactericidal concentration of LCEO to several foodborne bacteria were evaluated. The results indicated that gram‐negative bacteria (Salmonella, E. coli O157, E. coli DH5α, E.

Applications of whey protein concentrate (WPC) based films have been limited in the food industry due to their poor mechanical properties. This research aims to evaluate the effect of silver nanoparticles (AgNPs) synthesized by Aspergillus niger on the mechanical and antimicrobial properties of WPC based films. The biosynthesized AgNPs solution was added into the WPC film formula at the concentration of 0, 0.25 and 1.25 mM.

The genomes of three clinical isolates of Salmonella enterica subsp. houtenae were sequenced using an Illumina MiSeq instrument. These isolates came from the urine and cerebrospinal fluid of a dog treated for hind-limb paresis with immunosuppressive drugs. S. enterica subsp. houtenae has also been implicated in brain infections in humans.

Author(s): Paula de Paula Menezes Barbosa, Amanda Roggia Ruviaro, Isabela Mateus Martins, Juliana Alves Macedo, Gisèle LaPointe, Gabriela Alves Macedo

Author(s): Yin Wang, Xinyi Gou, Tianli Yue, Run Ren, Haoan Zhao, Liangliang He, Caiyun Liu, Wei Cao

Author(s): Tofa Begum, Peter A. Follett, Farah Hossain, Larry Christopher, Stephane Salmieri, Monique Lacroix

This study aimed to explore the stability of allyl isothiocyanate (AITC) after microencapsulation and its effect on the preservation of chilled fresh pork for 24 days. The single‐factor experiment and the orthogonal experiment were used to study the best experimental conditions (homogenizing time 4 min, stirring rate 900 rpm, Tween 80: core material volume ratio 1:20, and olive oil: AITC volume ratio 25:75).

Author(s): DanielF.M. Monte, Matthew A. Nethery, Rodolphe Barrangou, Mariza Landgraf, Paula J. Fedorka-Cray

Author(s): Bina Gautam, Byju N. Govindan, Michael Gӓnzle, M.S. Roopesh

Two monoclonal antibodies (mAbs) specific to Salmonella were produced from a mouse immunized with a combination of heat‐killed whole‐cell and formalin‐fixed Salmonella enterica serovar Enteritidis (S. Enteritidis) as the antigens. The mAb SE‐13D bound specifically only to S. Enteritidis, while mAb Sal‐06G bound to various members of the Salmonella serogroups B, C, D, E, and I. Neither mAb demonstrated any cross‐reactivity to other bacteria.

Poultry products such as eggs provide essential nutrients to the human body and thus play vital roles in the human food network. Salmonella is one of the most notorious foodborne pathogens and has been found to be prevalent in eggs. To better understand the characteristics of Salmonella in eggs, we investigated the prevalence of Salmonella spp. in 814 fresh eggs collected from poultry farms and retail marketplaces in Yangling, Shaanxi Province, China.