Research Publications (Food Safety)

The objective of this study was to explore the genetic and biological features of the tet(M)-harboring plasmid pTS14 in Salmonella enterica strain S14 isolated from a chicken fecal sample. Plasmid pTS14 was identified by conjugation, S1-pulsed-field gel electrophoresis (PFGE), Southern hybridization, and plasmid sequencing. The biological characteristics of pTS14 were assessed via stability, growth kinetics, and starvation survival experiments.

The aim of this research was to investigate the efficacy of the duty ratio and applied voltage in the inactivation of pathogens in soybean curd by pulsed ohmic heating (POH). The heating rate of soybean curd increased rapidly as the applied voltage increased, although the duty ratio did not affect the temperature profile. We supported this result by verifying that electrical conductivity increased with the applied voltage.

by Zhao Chen, Dai Kuang, Xuebin Xu, Narjol González-Escalona, David L. Erickson, Eric Brown, Jianghong Meng

by Csaba Varga, Patience John, Martin Cooke, Shannon E. Majowicz

Author(s): Xiao Fei, Qiuchun Li, John Elmerdahl Olsen, Xinan Jiao

Author(s): John A. Porter, Amit Morey, Emefa A. Monu

Pet food has been identified as a source of pathogenic bacteria, including Salmonella and Escherichia coli. A recent outbreak linked to Salmonella -contaminated pet treats infected over 150 people in the United States. The mechanism by which contaminated pet food leads to human illness has not been explicated. Pet owners’ food safety knowledge and their pet food handling practices have not been reported.

Food poisoning from consumption of food contaminated with non-typhoidal Salmonella spp. is a global problem. A modified high resolution DNA melting curve analysis (m-HRMa) was introduced to provide effective discrimination among closely related HRM curves of amplicons generated from selected Salmonella genome sequences enabled Salmonella spp. to be classified into discrete clusters.

Eudragit RS100 nanoparticles containing Baccharis dracunculifolia DC essential oil (N‐EO) was developed by the nanoprecipitation method. N‐EO presented an average diameter of 151.6 nm, zeta potential (ζ) of +51.7 mV, and encapsulation efficiency of 99.4%. The antibacterial activity of N‐EO and free essential oil (EO) was evaluated in BHI broth against Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, and Salmonella Enteritidis.

Author(s): Marianna M. Furtado, Beatriz S. Silva, César Faviero, Verônica O. Alvarenga, Anderson S. Sant’Ana

Antimicrobial resistance is one of today’s major public health challenges. Infections caused by multidrug-resistant bacteria have been responsible for an increasing number of deaths in recent decades. These resistant bacteria are also a concern in the food chain, as bacteria can resist common biocides used in the food industry and reach consumers. As a consequence, the search for alternatives to common antimicrobials by the scientific community has intensified.

One complication of malaria is increased susceptibility to invasive bacterial infections. Plasmodium infections impair host immunity to non-Typhoid Salmonella (NTS) through heme-oxygenase I (HO-I)-induced release of immature granulocytes and myeloid cell-derived IL-10. Yet, it is not known if these mechanisms are specific to NTS.

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ABSTRACT

Biofilms exist in complex environments including the intestinal tract as a part of the gastrointestinal microbiota. The interaction of planktonic bacteria with biofilms can be influenced by material properties of the biofilm. During previous confocal studies, we observed amyloid curli-containing Salmonella Typhimurium and Escherichia coli biofilms appeared rigid. In these studies, Enterococcus faecalis, which lacks curli-like protein, showed more fluid movement.

Hydrogen sulfide (H2S) detection is a screening method for distinguishing and identifying Salmonella strains from other bacteria in the intestine. Incidences of H2S-negative Salmonella have recently been reported in different countries.

The continuous collection and analysis of updated data on the antimicrobic resistance among bacterial strains represent the essential core for the surveillance of this problem. The present work aimed to investigate the occurrence of antimicrobial resistance among Salmonella serovars isolated in foods in 2015–2019. A total of 178 Salmonella strains belonging to 39 serovars were tested against 10 antimicrobials.

Author(s): Ghazaleh Salimi, Zeinab E. Mousavi, Hossein Kiani

Author(s): Ki-Hoon Lee, Ji-Young Lee, Pantu Kumar Roy, Furkanur Rahaman Mizan, Iqbal Hossain, Si Hong Park, Sang-Do Ha

Author(s): Tomi Obe, Rama Nannapaneni, Wes Schilling, Li Zhang, Chris McDaniel, Aaron Kiess

Author(s): Angela Parry-Hanson Kunadu, Richard Yaw Otwey, Lydia Mosi

Author(s): Anna Townsend, Shaoting Li, David A. Mann, Xiangyu Deng

Rat‐associated zoonoses transmitted through faeces or urine are of particular concern for public health because environmental exposure in homes and businesses may be frequent and undetected. To identify times and locations with greater public health risks from rats, we investigated whether rat characteristics, environmental features, socioeconomic factors, or season could predict rat infection risk across diverse urban neighbourhoods.

Author(s): Line Nielsen, Susanne Knøchel