Research Publications (Food Safety)

Author(s): Hye-Jin Seo, Seok-Seong Kang

Author(s): Mark Anthony B. Pelaez, Gerieka R. Anapi, Donna V. Bautista, Ma. Dulce P. Dallo, Joseph Conrad M. Libunao, Alonzo A. Gabriel

Aims The objective of this study was to evaluate the antimicrobial effects of Radio frequency (RF) heating and the combination treatment of RF heating with ultraviolet (UV) radiation against foodborne pathogens in roasted grain powder (RGP). Methods and Results Foodborne pathogens inoculated on RGP were subjected to RF heating or RF‐UV combination treatments. After 120 s of RF heating, 4.68, 3.89, and 4.54 log reductions were observed for E. coli, S. Typhimurium, and B.

Functional flagella formation is a widespread virulence factor that plays a critical role in survival and host colonization. Flagellar synthesis is a complex and highly coordinated process. The assembly of the axial structure beyond the cell membrane is mediated by export chaperone proteins that transport their cognate substrates to the export gate complex. The export chaperone FliS interacts with flagellin, the basic component used to construct the filament.

Author(s): Duc Kieu Minh, Yaovi M. Gildas Hounmanou, Huong Bui Thi Mai, John E. Olsen, Anders Dalsgaard

The ydcI gene has previously been shown to encode a DNA-binding protein involved with acid stress resistance and induced biofilm formation in a strain of Salmonella enterica serovar Typhimurium. In addition, characterisation of the ydcI gene in Escherichia coli and other bacteria demonstrated strikingly different tolerance for induced ydcI expression across Gram negative species.

Author(s): Qing Liu, Huali Su, Xiaoping Bian, Shifeng Wang, Qingke Kong

Author(s): Ke-Chuan Wang, Chih-Hung Huang, Pei-Ru Chang, Ming-Te Huang, Shiuh-Bin Fang

Author(s): Mary Muckey, Anne R. Huss, Ashton Yoder, Cassandra Jones

The food industry is considered to be one of the greatest sources of environmental contamination produced by dyes. Moreover, a large number of commercial food dyes and their by-products have been shown to be toxic, having chronic effects on human health. The search for efficient processes with which to treat these compounds is, therefore, necessary.

Salmonella enterica is the leading foodborne pathogen associated with outbreaks involving low-moisture foods (LMFs). However, the genes involved in Salmonella’s long-term survival on LMFs remain poorly characterized. In this study, in-shell pistachios were inoculated with Tn5-based mutant libraries of S. Enteritidis P125109, S. Typhimurium 14028s, and S. Newport C4.2 at approximate 108 CFU/g and stored at 25°C.

Salmonella enterica cause significant illnesses worldwide. There has been a marked increase in resistance to fluoroquinolones and β-lactams/cephalosporins, antibiotics commonly used to treat salmonellosis. However, S. enterica serovars vary in their resistance to these and other antibiotics. The systemic virulence of some Salmonella serovars is due to a low copy number, IncF plasmid (65–100 kb) that contains the ADP-ribosylating toxin, SpvB.

Salmonella Enteritidis is the most prevalent food-borne pathogen associated with egg-related outbreaks in the European Union. During egg colonization, S. Enteritidis must resist the powerful anti-bacterial activities of egg white (EW) and overcome ovotransferrin-imposed iron-restriction (the most important anti-bacterial mechanism of EW).

In this study, we investigated the pattern of antimicrobial resistance in Salmonella enterica serotype Enteritidis isolates in Shanghai, China from 2005 to 2014. We found the first isolates with resistance to the fourth-generation cephalosporin cefepime starting in 2010. Furthermore, we analyzed the epidemic characteristics and mechanisms of underlying cefepime resistance in S. Enteritidis isolates found from 2010.

The antibiotics generally used in farm animals are rapidly losing their effectiveness all over the world as bacteria develop antibiotic resistance. Like some other pathogenic bacteria multidrug-resistant strai...

ABSTRACT Aims This study measured the survival of Listeria monocytogenes and Salmonella enterica subsp. enterica serovar Javiana over a 10–day period on four soil‐free cultivation matrix (SFCM) types in the absence of microgreens and fertilizers. Methods and Results Coco coir (CC), a Sphagnum peat/vermiculite mix, Biostrate®, and hemp mat samples were inoculated with 3 x 106 CFU mL‐1 bacteria, incubated at room temperature, and analyzed on day 0, 1, 3, 6, and 10.

Salmonella enterica subsp. enterica serovar Derby is one of the most frequent causes of gastroenteritis in humans. In Europe, this pathogen is one of the top five most commonly reported serovars in human cases. In France, S. Derby has been among the ten most frequently isolated serovars in humans since the year 2000. The main animal hosts of this serovar are pigs and poultry, and white meat is the main source of human contamination.

Author(s): Mattika Abhisingha, Jureeporn Dumnil, Chetsadaporn Pitaksutheepong

L. Castellanos et al. Salmonella enterica serovar Paratyphi B variant Java sequence type 28 is prevalent in poultry and poultry meat. We investigated the evolutionary relatedness between sequence type 28 strains from Europe and Latin America using time-resolved phylogeny and principal component analysis. We sequenced isolates from Colombia, Guatemala, Costa Rica, and the Netherlands and complemented them with publicly available genomes from Europe, Africa, and the Middle East.

Author(s): Hong Qin, Xiuquan Shi, Liang Yu, Ke Li, Jianwu Wang, Jihua Chen, Fei Yang, Haiyan Xu, Huilan Xu

The main compounds of LC‐EO were citral and nerol. In the present study, gram‐negative bacteria had greater sensitivity to LC‐EO than gram‐positive bacteria. LC‐EO could lead to damage to the cell wall and membrane, resulting in the leakage of cell contents, such as electrolytes and nucleic acids, and eventual cell death. Abstract Litsea cubeba essential oil (LC‐EO) has been reported as antibacterial agents, but there are few studies about its possible antibacterial mechanism.

The emergence of antibiotic-resistant Salmonella through mutations led to mismatch repair (MMR) deficiency that represents a potential hazard to public health. Here, four representative MMR-deficient Salmonella hypermutator strains and Salmonella Typhimurium LT2 were used to comprehensively reveal the influence of MMR deficiency on antibiotic resistance among Salmonella. Our results indicated that the mutation frequency ranged from 3.39 × 10–4 to 5.46 × 10–2 in the hypermutator.

PDT is an emerging new antimicrobial technology, which uses a combination of photosensitizers and light. This study, for the first time, demonstrated that ERY (Red No. 3)‐mediated PDT can be an alternative antimicrobial treatment strategy to the conventional treatments used in tomato juice. Abstract The objectives of this study were to evaluate the efficacy of erythrosine B (ERY, Red No.

Author(s): Mariana Cap, Dante Rojas, Mariano Fernandez, Micaela Fulco, Anabel Rodriguez, Trinidad Soteras, Diego Cristos, Marina Mozgovoj