Research Publications (Food Safety)

Maize kernels contaminated with deoxynivalenol (DON) reduced their nutritional and economic values and were exposed to greater risks under high moisture conditions. In this study, an antifungal combination of bacillomycin D (BD) with calcium propionate (CP) was used in maize polluted by microorganisms and DON. During the storage of maize kernels, BD combined with calcium propionate (BD+CP) treatment inhibited the growth of fungi and bacteria.

The contamination of mycotoxin in edible oil has always been a major threat to human health. In this study, magnetic soybean dreg-based biochar SDB-6-K-9@Fe3O4 was prepared via co-precipitation and used to remove aflatoxin B1 (AFB1) from contaminated oil. The adsorbent characterization results revealed that the Fe3O4 was successfully loaded to the SDB-6-K-9.

The kinetics and thermodynamics of the enzymatic degradation of zearalenone (ZEN) in degummed corn oil were investigated by analyzing the impacts of temperature, pH, ZEN hydrolase dosage and ZEN concentration on the initial reaction rate. The kinetic study found that the maximum reaction rate was 0.97 μmol × kg−1 min−1, the Michaelis constant (Km) was 11,476 μmol × kg−1 and the Michaelis equation was V = 0.97[S]/(11,476 + [S]).

Aflatoxin B1 (AFB1) is widely prevalent in foods and animal feeds and is one of the most toxic and carcinogenic aflatoxin subtypes. Existing studies have proved that the intestine is targeted by AFB1, and adverse organic effects have been observed. This study aimed to investigate the relationship between AFB1-induced intestinal toxicity and N6-methyladenosine (m6A) RNA methylation, which involves the post-transcriptional regulation of mRNA expression.

Aflatoxin B1 (AFB1) that is prone to contaminate corns brings a serious threat to human health. Therefore, it is of great significance to construct novel detection methods for AFB1 tracing. Here, methylamine perovskite quantum dots (MP QDs) encapsulated by ZIF-8 metal-organic frameworks (MP QDs@ZIF-8) were prepared and then ultra-stable electrochemiluminescence (ECL) sensors were developed.

Herein, we conducted a comparative study on the embryotoxicity of ochratoxin A (OTA) and its diastereomer 2’R-ochratoxin A (2’R-OTA) under in ovo conditions, as well as assess the in vitro embryotoxicity of these substances together with ochratoxin B and α-ochratoxin, using chicken (Gallus gallus domesticus) embryo cell lines.

Fusarium trichothecenes are among the mycotoxins of most concern to food and feed safety. Production of these mycotoxins and presence of the trichothecene biosynthetic gene (TRI) cluster have been confirmed in only two multispecies lineages of Fusarium: the Fusarium incarnatum-equiseti (Incarnatum) and F. sambucinum (Sambucinum) species complexes. Here, we identified and characterized a TRI cluster in a species that has not been formally described and is represented by Fusarium sp.

A method was first developed based on MIL-101(Cr) dispersive solid-phase extraction for determining fumonisins in edible vegetable oil. Fumonisins contain hydroxyl and carboxyl groups and were easily adsorbed by MIL-101(Cr) with intermolecular force.

Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus spp. and Penicillium spp. that causes a threat to food safety and human health. Fungal biodegradation might be a promising strategy for reducing the OTA contamination in the future. In this study, the ability of Trichoderma koningii strains to degrade OTA produced by Aspergillus niger T2 (MW513392.1) isolated from tomato seeds was investigated. Among T.

A simple nanocomposite photocatalyst HT-rGO/TiO2 for deoxynivalenol (DON) degradation was synthesized by hydrothermal method to maintain the quality of cereal grains and byproducts. The characterization of HT-rGO/TiO2 was analyzed by XRD, FTIR, Raman spectroscopy, and XPS. Moreover, according to UV–vis DRS analysis, HT-rGO/TiO2 had a smaller band gap, indicating a wider response range to light and a higher utilized rate of quantum photons.

Aflatoxin, a carcinogenic secondary metabolite produced by Aspergillus flavus, is a significant threat to human health and agricultural production. Histone 2-hydroxyisobutyrylation is a novel post-translational modification that regulates various biological processes, including secondary metabolism. In this study, we identified the novel histone 2-hydroxyisobutyryltransferase Afngg1 in A. flavus, and explored its role in cell growth, development and aflatoxin biosynthesis.

Journal of the Science of Food and Agriculture, Accepted Article. Background Deoxynivalenol (DON) produced during the onset of fusarium head blight affects not only the quality and safety of wheat but also causes serious harm to human and livestock health. However, due to the high stability of DON, it is difficult to disappear or reduce naturally after production.

Background Exposure to zearalenone (ZEN, a widespread Fusarium mycotoxin) causes reproductive toxicity and immunotoxicity in farm animals, and it then poses potential threats to human health through the food chain. A systematic understanding of underlying mechanisms on mycotoxin-induced toxicity is necessary for overcoming potential threats to farm animals and humans.

The objective of this study was to investigate the effect of pulsed light (PL) treatment on the degradation of patulin in apple juice and its quality. The impact of different processing parameters on the degradation effect of patulin was explored. The degradation rate of patulin (200 μg/L) reached 96.27% in apple juice (12 oBrix) by applying the highest fluence (40.50 J/cm2, total processing time: 6 min 30 s) at 3.5 cm from the quartz glass.

Aflatoxin, a naturally occurring toxin produced by the fungus Aspergillus flavus, is the most economically important mycotoxin in the world, with harmful effects on human and animal health. Preventive measures such as irrigation and planting dates can minimize aflatoxin contamination most years. However, no control strategy is completely effective when environmental conditions are extremely favorable for growth of the fungus.

Patulin is a mycotoxin whose presence in apple-derived products and fruit juices is legally regulated, being its maximum limits established in the legislation of multiple countries. However, the management of contaminated batches is still an issue for producers. This investigation aims to evaluate ultraviolet light (254 nm, UV-C254nm) irradiation to find solutions that can be applied at different stages of the apple juice production chain.

Aflatoxin is a carcinogenic secondary metabolite that poses a serious threat to human and animal health. Some C2H2 transcription factors are associated with fungal growth and secondary metabolic regulation. In this study, we characterized the role of AflZKS3, a putative C2H2 transcription factor based on genome annotation, in the growth and aflatoxin biosynthesis of A. flavus and explored its possible mechanisms of action.

Type B trichothecenes commonly contaminate cereal grains and include five structurally related congeners: deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), fusarenon X (FX), and nivalenol (NIV). These toxins are known to have negative effects on human and animal health, particularly affecting food intake. However, the pathophysiological basis for anorexic effect is not fully clarified.

In this present study, 195 cow milk, 100 goat milk, 50 buffalo milk, 50 camel milk, and 50 yak milk samples were collected in China in May and October 2016. The presence of aflatoxin M1 (AFM1) was determined using enzyme-linked immunosorbent assay method.

A multicolor electrochemiluminescence (ECL) apparatus based on a closed bipolar electrode (BPE) system was proposed for the visual testing of Ochratoxin A (OTA). Since the oxidation potentials of the accompanying luminophores ([Ru(bpy)3]2+ and [Ir(ppy)3]) are different, a bidirectional color change from dark orange to yellow to forest green can be discovered at the anode of BPE by adjusting the current of the BPE.

Ochratoxin A (OTA) with high toxicity represents a serious threat to the agriculture and food chain, consequently to human health. Herein, a simple electrochemiluminescence (ECL) biosensor was constructed for ultrasensitive detection of OTA based on mercaptopropionic acid templated Au nanoclusters (Au NCs) as intensive signal probe and a non-enzymatic 2D DNA walking machine as the effective amplification strategy.

The reaction efficiency of surface-based DNA walker can directly affect the properties of a biosensor. Herein, three-dimensional (3D) DNAzyme walker were first fixed on the top of DNA tetrahedral scaffold to improve the immobilization efficiency. Ferrocene (Fc) that labeled at substrate strand ends effectively quenched the electrochemiluminescence (ECL) signal of Ru(bpy)2(cpaphen)2+, yielding the sensor in a “signal-off” state.

Among a few hundred mycotoxins, aflatoxins had always posed a major threat to the world. Apart from A. flavus, A. parasiticus, and A. nomius of Aspergillus genus, which are most toxin-producing strains, several fungal bodies including Fusarium, Penicillium, and Alternaria that can biosynthesis aflatoxins. Basically, there are four different types of aflatoxins (Aflatoxin B1 (AFB1), Aflatoxin B2 (AFB2), Aflatoxin G1 (AFG1), Aflatoxin G2 (AFG2)) are produced as secondary metabolites.

Inspired by the super-binding capacity of pyrophosphate (ppi) and copper ion (Cu2+), an alkaline phosphatase (ALP) triggered ratiometric fluorescence immunoassay was established for detection of zearalenone (ZEN). In the assay, ALP-antibody acted as a bridge connecting ZEN concentration and ratiometric fluorescence signals. Due to the super-binding ability of ppi and Cu2+, Cu2+ was immobilized to form ppi-Cu2+ complex and lost its oxidation activity.

Aflatoxin B1 (AFB1) is a mycotoxin synthesised as a secondary metabolite by members of the Aspergillus species contaminating agricultural produce. Aspergillus species thrive in tropical climes, endemic to malaria. Artemisinin-based combination therapies (ACTs) effectively treat and prevent malaria recrudescence; Coartem (COA) is an ACT whose toxicity is evident.