Research Publications (Food Safety)

Author(s): Panpan Hu, Na Sun, Ajab khan, Xinyue Zhang, Panpan Sun, Yaogui Sun, Jianzhong Guo, Xiaozhong Zheng, Wei Yin, Kuohai Fan, Jianzhong Wang, Huizhen Yang, Hongquan Li

Author(s): Kun Ma, Enjie Diao, Hui Zhang, Shiquan Qian, Peng Xie, Ruifeng Mao, Huwei Song, Liming Zhang

A specialized method for ochratoxin A (OTA) determination on fermented teas was developed and validated using ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Methodology results showed that recovery, relative standard deviation, accuracy, and precision were qualified. The limits of detection and quantification were 0.32 and 0.96 μg/kg, respectively. Two of 158 collected samples were screened for OTA contamination.

The contamination of mycotoxin zearalenone (ZEN) in foods has been reported worldwide, resulting in potential risks to food safety. However, the toxic mechanism of ZEN on neurodegenerative diseases has not been fully elucidated. Therefore, this study conducted in vivo ZEN neurotoxicity assessment on Parkinson’s disease (PD)-related dopaminergic neurodegeneration and mitochondrial dysfunction using Caenorhabditis elegans.

Zearalenone (ZEA) is a mycotoxin that contaminates cereals and grains. Atmospheric cold plasma (ACP) is a novel non-thermal technology that has the potential to degrade mycotoxins. This study evaluated the potential of ACP and the influence of some of the important product and process factors on the degradation of ZEA. The efficacy of 30 s dielectric barrier discharge (DBD) ACP treatment to degrade ZEA in solution and dry condition was 100% and 66.8%, respectively.

The consumption of pistachios (Pistacia vera L.) has been increasing, given their important benefit to human health. In addition to being an excellent nutritional source, they have been associated with chemical hazards, such as mycotoxins, resulting in fungal contamination and its secondary metabolism. Aflatoxins (AFs) are the most common mycotoxins in pistachio and the most toxic to humans, with hepatotoxic effects.

Climate change will increase the co-occurrence of Fusarium verticillioides and Aspergillus flavus, along with their mycotoxins, in European maize. In this study, the expression profiles of two pathogenesis-related (PR) genes and four mycotoxin biosynthetic genes, FUM1 and FUM13, fumonisin pathway, and aflR and aflD, aflatoxin pathway, as well as mycotoxin production, were examined in kernels and in artificial medium after a single inoculation with F. verticillioides or A.

Author(s): Jalila Ben Salah-Abbès, Marwa Mannai, Hela Belgacem, Abdella Zinedine, Samir Abbès

Ochratoxins are mycotoxins that have been extensively studied lately due to the multiple toxic effects such as nephrotoxicity, hepatotoxicity, and carcinogenicity. These toxins contaminate plant and animal foods and after ingestion they reach into body fluids. The method of competitive direct enzyme immunoassay, in the solid phase, was validated through the determination of specific parameters (performance, linearity, recovery percentage, limit of detection, limit of quantification).

Author(s): Hong-Yun Zhang, Ya-Li Wang, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Lu Zhang, Hai-Feng Mi, Jun Jiang, Sheng-Yao Kuang, Ling Tang, Lin Feng

Our previous study showed that ochratoxin A (OTA), one of the most common mycotoxins in feed, could induce immunosuppression with long-time exposure but immunostimulation with short-time exposure. However, limited studies for the control of OTA-induced two-way immune toxicity were carried out.

Journal of Separation Science, Accepted Article. Zearalenone is a fungal contaminant that is widely present in grains. Here, a novel molecularly imprinted membrane based on SOM-ZIF-8 was developed for the rapid and highly selective identification of zearalenone in grain samples. The molecularly imprinted membrane was prepared using polyvinylidene fluoride, cyclododecyl 2,4-dihydroxybenzoate as a template and SOM-ZIF-8 as a carrier.

A novel multiplex mycotoxin surface-enhanced Raman spectroscopy (SERS) immunoassay was established for the first time on different artificial antigen-modified silica photonic crystal microspheres (SPCMs), which can be integrated into a biochip array to achieve multiplex detection using corresponding antibody-functionalized gold nanoparticles (AuNPs) as the SERS nanotag.

New information is needed regarding the types and concentrations of mycotoxins in swine feed. We hypothesized that (1) the mycotoxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), nivalenol (NIV), and zearalenone (ZEN) vary among swine ingredient and feed types, and (2) the inclusion of specific ingredients is associated with mycotoxin contamination in complete feed.

The current study aimed to evaluate the impact of the crop season, cultivation region, and traditional pre- and post-harvest agricultural practices on mycotoxin contamination in the Mekong Delta rice chain of Vietnam. The results showed that aflatoxins (AFs) and fumonisins (FBs) were predominantly detected in both paddy (n = 91/184, 50%) and white rice (n = 9/46, 20%). Aflatoxin B1 (AFB1)-contaminated paddy samples (n = 3) exceeded the regulatory threshold (5 µg·kg−1).

Author(s): Mi-Na Nan, Yang Bi, Yao Qiang, Hua-Li Xue, Lan Yang, Li-Dan Feng, Lu-Mei Pu, Hai-Tao Long, Dov Prusky

Author(s): Yuanliang Li, Jiangnan Yi, Qiwen Zeng, Yingwei Liu, Bijing Yang, Bingxian Liu, Yangwei Li, Khalid Mehmood, Riaz Hussain, Zhaoxin Tang, Hui Zhang, Ying Li

Author(s): Yuhan Sun, Yan Lv, Shuo Qi, Yin Zhang, Zhouping Wang

The thermal stability and degradation kinetics of patulin (PAT, 10 μmol/L) in pH 3.5 of phosphoric-citric acid buffer solutions in the absence and presence of cysteine (CYS, 30 μmol/L) were investigated at temperatures ranging from 90 to 150 °C. The zero-, first-, and second-order models and the Weibull model were used to fit the degradation process of patulin.

Latin America with its considerable North-South extent is subject to climate that varies from tropical, subtropical and warm temperate to temperate. Different agricultural products are produced in the area including cereals, oilseeds, beans, fruits and nuts together with animal production including cattle for beef and milk, pigs, poultry and fish. The heterogeneity of agriculture in Latin America is reflected in the diversity of the region’s farm structures.

Two simple and low-cost QuEChERS approaches were optimized and validated for multimycotoxin determination in grains by UPLC–MS/MS and applied to assess effectiveness of controlled atmosphere (CA) storage in preventing mycotoxin contamination. Common bean, soybean, and maize samples were stored for 6 months. CA treatments were conducted varying O2 and CO2 partial pressures, temperatures, and moisture contents of the chambers.

Author(s): Yifang Zeng, María Belén Camarada, Xinyu Lu, Kaijie Tang, Weiqiang Li, Daoyang Qiu, Yangping Wen, Guoping Wu, Qiushui Luo, Ling Bai

The occurrence of mycotoxins on grapes poses a high risk for food safety; thus, it is necessary to implement effective prevention methods. In this work, a metagenomic approach revealed the presence of important mycotoxigenic fungi in grape berries, including Aspergillus flavus, Aspergillus niger aggregate species, or Aspergillus section Circumdati. However, A. carbonarius was not detected in any sample.

The purpose of this study was to investigate the combined effects of aflatoxin B1 and ochratoxin A on protein expression and catalytic activities of CYP1A2, CYP2E1, CYP3A29 and GSTA1 and the preventive effect of dietary byproduct antioxidants administration against these mycotoxin damage. Three experimental groups (E1, E2, E3) and one control group (C) of piglets after weaning (TOPIGS-40 hybrid) were fed with experimental diets for 30 days.

This study characterized the health risks due to the consumption of mycotoxin-contaminated foods and assessed the consumer awareness level of mycotoxins in households in two north-central Nigerian states during the harvest and storage seasons of 2018. Twenty-six mycotoxins and 121 other microbial and plant metabolites were quantified by LC-MS/MS in 250 samples of cereals, nuts and legumes. Aflatoxins were detected in all food types (cowpea, maize, peanut and sorghum) except in millet.