Research Publications (Food Safety)

Ergot alkaloids are important specialized fungal metabolites that are used to make potent pharmaceuticals for neurological diseases and disorders. Lysergic acid (LA) and dihydrolysergic acid (DHLA) are desirable lead compounds for pharmaceutical semisynthesis but are typically transient intermediates in the ergot alkaloid and dihydroergot alkaloid pathways.

Biphenyl dioxygenase (BPDO), which is a Rieske-type oxygenase (RO), catalyzes the initial dioxygenation of biphenyl and some polychlorinated biphenyls (PCBs). In order to enhance the degradation ability of BPDO in terms of a broader substrate range, the BphAES283M, BphAEp4-S283M, and BphAERR41-S283M variants were created from the parent enzymes BphAELB400, BphAEp4, and BphAERR41, respectively, by a substitution at one residue, Ser283Met.

Author(s): Vijakhana Pilaisangsuree, Piyanuch Anuwan, Kanitha Supdensong, Pimpimon Lumpa, Anupan Kongbangkerd, Apinun Limmongkon

Author(s): Antonio León-Vaz, Luis C. Romero, Cecilia Gotor, Rosa León, Javier Vigara

Author(s): Pengwei Yao, Hanjun Zhou, Xueli Li, Lin Wei, Jing Wang, Sheng Zhang, Xiefeng Ye

In this study, total RNA was extracted from young leaves of maize. Reverse transcription‐PCR (RT‐PCR) method was used to obtain full‐length TGase gene. The amplified fragment was sequenced to have 1,605 bp. The gene consisted of 535 amino acid residues with a calculated molecular mass of 60.9 kD. It was identical to the published TGase gene (GenBank NO.AJ421525). The TGase fragment was cloned into pET‐28a prokaryotic expression vector.

Cadmium (Cd) is a common toxic heavy metal in the environment, and bacteria have evolved different strategies against Cd‐toxicity. Here we found that marine bacterium Bacillus sp. 98 could significantly alleviate Cd‐toxicity by recruiting calcium (Ca) for reducing excessive intracellular nitric oxide (NO) and enhancing iron acquisition. To investigate the underlying mechanisms, mass spectrometry‐based proteomic analysis was applied to Bacillus sp.

Bisphenol A (BPA), an imperative environmental contaminant used in polycarbonate plastics. Due to limited information concerning the effect of BPA on lungs, this study design to assess whether BPA cause alterations in histopathology and trace metal content in lungs of rats. They were divided into five groups with five rats per group. Group I was named as control group. Group L6 and L12 were received BPA (10 mg/kg body weight/day) for 6 weeks and 12 weeks respectively.

Fungi have increased tolerance to environmental stress (also related to the access of pollutants, e.g., trace elements and polycyclic aromatic hydrocarbons PAHs). The aim of the study was to evaluate the mycobiome and functional diversity of fungi in long-term crude-oil contaminated soils as the potential bioremediators of oil contaminated sites.

Salicornia ramosissima is a C3 halophyte that grows naturally in South Western Spain salt marshes, under soil salinity and heavy metal pollution (mostly Cu, Zn, As, and Pb) caused by both natural and anthropogenic pressure. However, very few works have reported the phytoremediation potential of S. ramosissima. In this work, we studied a microbe-assisted phytoremediation strategy under greenhouse conditions.

Author(s): Xiao-long Hou, Hang Han, Mulualem Tigabu, Qi-yan Li, Zong-xun Li, Chen-lu Zhu, Si-qi Huang, Li-ping Cai, Ai-qin Liu

The distribution and composition of polychlorinated biphenyls (PCBs) within soil–plant systems around a notorious e-waste recycling site were investigated. The average total PCB concentrations in rhizospheric soils (RSs) and nonrhizospheric soils (NRSs) were 2160 and 1270 pg g–1 dry weight (DW), respectively. PCBs were more enriched in RS than NRS for most vegetable species.

Contamination of food and feed items with mycotoxins causes extensive economic damage. It is therefore important to explore environmentally friendly approaches to manage these toxins with less drawbacks. Phytochemicals can provide a safe alternative to synthetic chemicals. This study was designed to investigate the detoxification potential of water-based extracts of Acacia nilotica against aflatoxins B1 and B2.

Pesticide and veterinary drug residues in food and environment pose a threat to human health, and a rapid, super-sensitive, accurate and cost-effective analysis technique is therefore highly required to overcome the disadvantages of conventional techniques based on mass spectrometry.

The mechanism of aluminum toxicity was studied in the model cells of Saccharomyces cerevisiae. Cell growth of yeast was inhibited by aluminum. The spot assay showed that the mechanism of aluminum detoxification in yeast cells was different from that of heavy metal cadmium. After treatment with aluminum, intracellular levels of reactive oxygen species, protein carbonyl, and thiobarbituric acid reactive substances were dramatically increased.

Lentinula edodes, also known as Xiang’gu, is commonly eaten in cultures around the world. However, L. edodes is particularly susceptible to enrichment with heavy metals, particularly cadmium (Cd), which is toxic to human health. Understanding the molecular mechanism and mining key genes involved in Cd enrichment will facilitate genetic modification of L. edodes strains. Two L.

Apilactobacillus kunkeei FF30-6 isolated from healthy honey bees synthesizes the bacteriocin, which exhibits antimicrobial activity against Melissococcus plutonius. The bacteriocin, kunkecin A, was purified through three-step chromatography, and mass spectrometry revealed that its relative molecular mass was 4218.3. Edman degradation of purified kunkecin A showed only the N-terminal residue, isoleucine.

Inorganic arsenic (iAs) is a recognized environment-related factor for bladder cancer (BCa). Arsenic (+3 oxidation state) methyltransferase (AS3MT) gene might influence BCa by regulating iAs metabolism. The aim of the present study was to explore whether AS3MT polymorphisms could affect BCa susceptibility.

Polychlorinated biphenyls (PCBs) are ubiquitously detected and have been linked to metabolic diseases. Gut microbiome is recognized as a critical regulator of disease susceptibility; however, little is known how PCBs and gut microbiome interact to modulate hepatic xenobiotic and intermediary metabolism. We hypothesized the gut microbiome regulates PCB-mediated changes in the metabolic fingerprints and hepatic transcriptome.

Drug-induced liver injury is a major reason for drug candidate attrition from development, denied commercialization, market withdrawal, and restricted prescribing of pharmaceuticals. The metabolic bioactivation of drugs to chemically reactive metabolites (CRMs) contribute to liver-associated adverse drug reactions in humans that often goes undetected in conventional animal toxicology studies.

Toxicological Sciences, 164(2), 2018, 527–538

Cadmium (Cd) is a heavy metal and an environmental pollutant. However, the full spectrum of its neurotoxicity and the underlying mechanisms are not completely understood. Our previous studies demonstrated that Cd exposure impairs adult hippocampal neurogenesis and hippocampus-dependent memory in mice. This study aims to determine if these adverse effects of Cd exposure can be mitigated by genetically and conditionally enhancing adult neurogenesis.

Heavy metals are typical cumulative pollutants that can enter and poison the human body through the food chain. However, the molecular mechanism of heavy metal-induced oxidative stress is unclear. In this study, we characterize PvKelch-like-1 from P. vannamei and explore its antioxidant roles in immune regulation of crustaceans.

Lead–zinc (Pb–Zn) mine tailings pose a great risk to the natural environment and human health because of their high toxicity. In this study, the responses of photosynthesis, chlorophyll fluorescence, and antioxidative enzyme of Melia azedarach and Ligustrum lucidum in the soil contaminated by Pb–Zn mine tailings were investigated.

In this work, we report the isolation and detailed functional characterization for the new non-ribosomally synthesized antibiotic 5812-A/C, which was derived from metabolites of Streptomyces roseoflavus INA-Ac-5812. According to its chemical structure, the studied 5812-A/C preliminary is composed of a cyclic peptide part covalently bounded with an arabinose residue.