Research Publications (Food Safety)

Abstract Aims Effects of H2O2 producing electrochemical‐bandages (e‐bandages) on methicillin‐resistant Staphylococcus aureus colonization and biofilm removal were assessed using a porcine explant biofilm model. Transport of H2O2 produced from the e‐bandage into explant tissue and associated potential toxicity were evaluated. Methods and Results Viable prokaryotic cells from infected explants were quantified after 48 h treatment with e‐bandages in three ex vivo S.

Journal of Applied Microbiology, Accepted Article. Aims The aim of this study was to develop a novel approach using lateral flow recombinase polymerase amplification (RPA-LF) combined with immunomagnetic separation (IMS) for the rapid detection of Staphylococcus aureus in milk. Methods and results Under optimum conditions, the average capture efficiency values (CEs) for S.

Journal of Applied Microbiology, Accepted Article. The molecular ecology of Staphylococcus aureus, Staphylococcus pseudintermedius, and their methicillin-resistant strains in healthy dogs and cats could serve as good models to understand the concept of bacterial zoonosis due to animal companionship. This study aims to provide insights into pooled prevalence, genetic lineages, virulence, and antimicrobial resistance (AMR) among healthy dogs and cats.

Journal of Applied Microbiology, Accepted Article. Aim To evaluate the antimicrobial capability of sclareol and its derivatives against Staphylococcus aureus and its Methicillin-resistant strain (MRSA). Methods and Results A new series of Boron-Heck-coupled sclareol analogs were prepared by structural modifications at C-15 terminal double bond of sclareol using ultrasonication.

Journal of Applied Microbiology, Accepted Article. Aims Antimicrobial resistance is one of the highest priorities in global public health with Staphylococcus aureus among the most important microorganisms due to its rapidly evolving antimicrobial resistance. Despite all the efforts of antimicrobial stewardship, research and development of new antimicrobials are still imperative.

Journal of Applied Microbiology, Accepted Article. Aims Bacterial response to temperature changes can influence their pathogenicity to plants and humans. Changes in temperature can affect cellular and physiological responses in bacteria that can in turn affect the evolution and prevalence of antibiotic-resistance genes.

Journal of Applied Microbiology, Accepted Article. Aims Staphylococcus aureus has emerged as a serious threat to food safety owing to biofilm formation. The study aimed to examine antibiofilm mechanism of a novel milk-derived antimicrobial peptide BCp12 against it. Methods and results Anti-biofilm activity of BCp12 was studied by crystal violet staining, MTT assay, motility, SEM and CLSM.

Aims

Dry surface biofilms (DSB) survive on environmental surfaces throughout hospitals, able to resist cleaning and disinfection interventions. This study aimed to produce a dual species DSB and explore the ability of commercially available wipe products to eliminate pathogens within a dual species DSB and prevent their transfer.

Methods and Results

Journal of Applied Microbiology, Accepted Article. Aims To evaluate the role of starter inoculums to reduce total fermentation time as well as improve safety and quality of Sheedal, a fermented fish-product of India. Methods and Results Lactiplantibacillus plantarum and Staphylococcus piscifermentans were isolated from the Sheedal and used as starter inoculums.

Journal of Applied Microbiology, Accepted Article.  Aim To assess the biofilm-producing capacities of S. aureus strains isolated from hospitalized patients in Israel. Methods and Results A total of 163 S. aureus (80 MRSA and 83 MSSA) screening (nasal swab) and clinical samples (blood and wounds) were characterized.

Journal of Applied Microbiology, Accepted Article. Aim The emergence of vancomycin-resistant Staphylococcus aureus (VRSA) has been identified as one of the most challenging problems in healthcare settings worldwide. Specific conjugation inhibitors development are critical in the fight against the spread of emerging VRSA.

Journal of Applied Microbiology, Volume 132, Issue 3, Page 2331-2341, March 2022. Aim This study aimed to identify genotype enterotoxigenic antimicrobial-resistant Staphylococcus aureus species, mainly methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus (VRSA) among commensal rodents. Methods and results A total of 280 samples were collected from nasal and mouth swabs

Journal of Applied Microbiology, Volume 132, Issue 3, Page 1840-1855, March 2022. Aims Because the Staphylococcus aureus is one of the most well-known pathogens associated with medical devices and nosocomial infections, the aim of the study was to examine antibiofilm potential of emodin against it. Methods and Results Antibacterial activity was examined through microdilution assay.

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1422-1434, February 2022. Aims Staphylococcus aureus is an opportunistic pathogen of humans. No commercial vaccine is available to combat S. aureus infections. In this study, we have investigated the protective immune response generated by S.

Journal of Applied Microbiology, Accepted Article. Aim Bacteria naturally produce membrane vesicles (MVs), which have been shown to contribute to the spread of multi-drug resistant bacteria (MDR) by delivering antibiotic-resistant substances to antibiotic-susceptible bacteria.

Journal of Applied Microbiology, Volume 132, Issue 1, Page 140-154, January 2022.

Journal of Applied Microbiology, Volume 132, Issue 1, Page 209-220, January 2022.

Journal of Applied Microbiology, Accepted Article.

Journal of Applied Microbiology, Accepted Article. Aims
To phytosynthesize silver nanoparticles (AgNPs) and determine their antibacterial and antibiofilm capacity against gram-positive and negative bacterial strains.

Journal of Applied Microbiology, Accepted Article. The purpose of this study was to detect growth enhancing or inhibiting activity between bacterial populations from raw milk under different conditions (temperature, medium).

Aims To perform the molecular characterization of 23 Staphylococcus aureus isolates from pigs with signs of infections recovered in Spanish farms during 2018‐2019. Methods and Results The antimicrobial resistance pattern and virulence profile were determined. The molecular typing was performed by different molecular techniques. The transferability of the cfr gene was assessed by conjugation and its genetic environment was determined by PCR‐mapping.

Staphylococcus aureus, an opportunistic pathogen, causes diverse community and nosocomial acquired human infections, including folliculitis, impetigo, sepsis, septic arthritis, endocarditis, osteomyelitis, implant associated biofilm infections and contagious mastitis in cattle. In recent days both methicillin‐sensitive and methicillin resistant S. aureus infections have increased. Highly effective anti‐staphylococcal agents are urgently required.

Aims To evaluate the applicability of the Mimosa tenuiflora and Eucalyptus urograndis pyroligneous acids (PAs) as alternative antiseptics in dairy goats. Methods and Results Cytotoxicity was evaluated in vitro using bacteria, as well as in vivo using goats, and the influence of PAs on the physicochemical parameters of fresh milk were examined. The cytotoxicity of PAs was evaluated in terms of morphology, cell viability, and metabolic activity of goat tegumentary cells. The PA of M.

Aims In previous work, following a pressure treatment of S. aureus wild type, we obtained piezotolerant isolates showing altered phenotypic characteristics. This work focuses on understanding the genetic background of their altered phenotype. Methods and Results AK23, a representative piezotolerant isolate was subjected to DNA microarrays, corroborated by PCR product sequencing and revealed 10‐gene deletion.

Aim Bacteriocins with antimicrobial activity are considered as potential natural bio‐preservatives to control the growth of food spoilage bacteria. The aim of this work is to characterize a novel bacteriocin BM1029 discovered from Lactobacillus crustorum MN047 and evaluate its antibacterial mechanism. Methods and Results Bacteriocin BM1029 was purified by cation‐exchange chromatography and reversed‐phase chromatography.