Research Publications (Food Safety)

A method for the rapid screening and confirmation of 60 food‐borne stimulants in animal‐derived foods was developed using the ultra‐high‐performance liquid chromatography‐quadrupole/orbitrap high‐resolution mass spectrometry (UPLC‐Q/Orbitrap HRMS). After enzymatic hydrolysis at 37°C for 16 h, the samples were extracted with acetonitrile solution. The extraction solution was purified by PRiME HLB pass‐through solid‐phase extraction cartridge and redissolved by nitrogen blowing.

The impact of soil fertilization with animal manure on the spread and persistence of antibiotic resistance in the environment is far from being fully understood. To add knowledge about persistence and correlations between antibiotic residues and antibiotic resistance genes (ARGs) in fertilized soil, a longitudinal soil mesocosm study was conducted.

by Daniel Ciccarone, Nicole Holm, Jeff Ondocsin, Allison Schlosser, Jason Fessel, Amanda Cowan, Sarah G. Mars

Background

Illicitly manufactured fentanyls and stimulants are implicated in the escalating US mortality from drug overdose. San Francisco, California (SF) has seen declining fentanyl injection while smoking has increased. Beliefs and behaviors surrounding this development are not well understood.

Methods

Probiotic feed additive use in the swine industry has become increasingly popular as a substitute for antibiotic feed additives. This is due to the overuse of antibiotics in swine diets that has led to antibiotic residues in animal products and bacterial resistance. For this reason, we decided to feed a probiotic to prevent illnesses associated with the transition from the nursery to finisher phase.

Sulfonamide antibiotics with superior performance are widely used in aquaculture. However, the residues of sulfonamide antibiotics in aquatic products may pose a threat to human health. In this work, a novel type of magnetic molecularly imprinted polymer (mMIP) was synthesized and coupled with ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) for the selective separation and determination of 4 sulfonamide antibiotics residues in tilapia.

From a public health point of view, antimicrobial residues pose various problems, including potential risks for consumers. The use of some of these antibiotics may affect the form and quality of animal products or affect the quality or output of food processing. Consequently, there is a pressing need for further investigation into the effects of antibiotics on animal products.

IntroductionControl of Campylobacter from farm to fork is challenging due to the frequent emergence of antimicrobial-resistant isolates. Furthermore, poultry production systems are known reservoirs of Campylobacter. The twin-arginine translocation (Tat) pathway is a crucial bacterial secretion system that allows Campylobacter to colonize the host intestinal tract by using formate as the main source of energy.

An HPLC-MS/MS multi-class method for quantitation of 15 different classes of veterinary drug residues (>140 analytes) in milk and poultry feed was developed and validated. Accuracy criteria for routine laboratories were met for the majority of analytes, > 83 % in milk and between 50 and 60 % in chicken feed, with an apparent recovery of 60–140 %.

Abstract

Abstract The importance of food quality and safety lies in ensuring the best product quality to meet consumer demands and public health. Advanced technologies play a crucial role in minimizing the risk of foodborne illnesses, contamination, drug residue, and other potential hazards in food. Significant materials and technological advancements have been made throughout the food supply chain.

β-Lactam is one of the widely used veterinary drugs, but simultaneous analytical methods for β-lactam on various animal foods have not been established. In this study, we aimed to detect 34 β-lactam antibiotics simultaneously in livestock samples (beef, pork, chicken, egg, and milk) by liquid chromatography-tandem mass spectrometry (LC–MS/MS).

Aristolochic acid nephropathy (AAN) has drawn increasing public attention. Organic anion transporters (OATs) are considered to be responsible for mediating nephrotoxicity of aristolochic acids (AAs), as AAs are typical OAT1 substrates that exhibit anionic properties and contain one hydrophobic domain.

Background The consumption of foods containing amphenicols, a type of antibiotic, is a major concern for human health. A stable and accurate detection method can provide technical support for food-safety monitoring. Objective An effective and efficient method was established for determining amphenicols in animal-derived foods through the simultaneous use of solid-phase extraction (SPE) cleanup and ultrahigh-performance liquid chromatography/mass spectrometry (UPLC-MS/MS).

The excessive and uncontrolled application of antibiotics in the fish farming industry, coupled with a lack of health monitoring and medication practices, is a driving force behind the escalating development of antimicrobial resistance. The present study assessed and compared qualitative field diffusion (QFD) and disk diffusion (DD) assays for the detection of antimicrobial residues (ARs) in diverse freshwater aquaculture fish.

Although filtration is one of the most common steps in sample preparation for chemical analysis, filter membrane materials can leach contaminants and/or retain some analytes in the filtered solutions. In multiclass, multiresidue analysis of veterinary drugs, it is challenging to find one type of filter membrane that does not retain at least some of the analytes before injection in ultrahigh‐performance liquid chromatography‐tandem mass spectrometry (UHPLC‐MS/MS).

The aim of this study was to compare the results of the presence of antibiotic (AB) residues in the milk of dairy cows treated with ABs – during the withdrawal period (WP) and after the WP obtained by various screening tests (Delvotest T, Twinsensor BT020, and Eclipse 50), to evaluate the presence of AB residues with regard to selected factors (indication for AB administration, AB group, and a number of antimicrobial substances) and to assess selected parameters of milk quality (fat, crude pr

Pigeon is an essential poultry with tasty meat and high protein content, and its breeding and consumption of meat have been increasing annually. Nevertheless, technical bottlenecks still exist in detecting veterinary drug residues in pigeon muscle. A rapid and sensitive method for determining 67 veterinary drugs belonging to six major groups (quinolones, sulfonamides, macrolides, hormones, tetracyclines, and insecticides) was developed in pigeon muscle.

Antibiotic resistance genes (ARGs) are emerging pollutants that enter the farm and surrounding environment via the manure of antibiotic-treated animals. Pretreatment of livestock manure by composting decreases ARGs abundance, but how antibiotic residues affect ARGs removal efficiency remains poorly understood.

Gastrointestinal nematodes (GINs) can be a major constraint and global challenge to the sheep industry. These nematodes infect the small intestine and abomasum of grazing sheep, causing symptoms such as weight loss, diarrhea, hypoproteinemia, and anemia, which can lead to death. The use of anthelmintics to treat infected animals has led to GIN resistance, and excessive use of these drugs has resulted in residue traced in food and the environment.

The indiscriminate use of antibiotics in agriculture has raised concerns about antibiotic residues in food products, necessitating robust analytical methods for detection and quantification. In this study, our primary aim was to develop a robust and advanced liquid chromatography-tandem mass spectrometry (LC-MS/MS) methodology specifically designed for the accurate quantification of ticarcillin degradation products in tomato leaves.

This study investigated antibiotic utilization in artisanal dairies and residue occurrence throughout the raw milk cheese production chain using commercial testing (Charm KIS and Eclipse Farm3G) and UHPLC-QqQ-MS/MS and LC-QqQ-MS/MS. The cross-sectional survey results revealed gaps in the producers’ knowledge of antibiotic use.

The symbiotic community of microorganisms in the gut plays an important role in the health of the host. While many previous studies have been performed on the interactions between the gut microbiome and the host in mammals, studies in fish are still lacking. In this study, we investigated changes in the intestinal microbiome and pathogen susceptibility of zebrafish (Danio rerio) following chronic antibiotics exposure.

Background An LC-MS/MS method was developed for determination and confirmation of tilmicosin in bovine, swine, chicken, and turkey tissues (liver, kidney, muscle, and skin/fat) and bovine milk. Objective The method was subjected to single-laboratory validation to establish method performance parameters.

In Ecuador, more than 50% of the milk produced was informally marketed, consequently, the mean of this study was to assess the quality of this milk specifically within the province of Pichincha, the largest milk producer. A total of 650 samples were gathered from the eight cantons within the province.