Research Publications (Food Safety)

Aquaculture is a crucial industry that can help meet the increasing demand for aquatic protein products and provide employment opportunities in coastal areas and beyond. If incorrectly manage, traditional aquaculture methods can have negative impacts on the environment and natural resources, including water pollution and overuse of wild fish stocks as aquafeed ingredients.

This study investigated the effects of varying doses of dietary aflatoxin B1 (AFB1) on the growth, intestinal health, and muscle quality of hybrid grouper. Four diets with varying AFB1 concentrations (0, 30, 445, and 2,230 μg kg−1) were used. Elevating AFB1 concentrations led to a decline in growth indexes, specifically the weight gain rate and the specific growth rate, although the survival rate remained unchanged. Morphological indicators showed a dose‐dependent decline with AFB1 exposure.

Ocimum basilicum has antioxidant, anti‐inflammatory, and antimicrobial effects. The present study was conducted to evaluate the effects of O. basilicum extract on growth yield, safety, and marinating physiologic functions of the rainbow trout. The fish were fed with food rations containing 0%, 1%, 2%, and 3% of basil extract. Basil extract, especially at 1% concentration food ration, significantly increased the growth parameters compared with the control group (p < 0.05).

Infection with the pathogenic bacterium Vibrio parahaemolyticus typically causes severe hepatopancreatic damage in Penaeus vannamei, often resulting in acute shrimp mortality. Therefore, protecting the shrimp’s hepatopancreas is crucial for enhancing their disease resistance. Previous research has demonstrated that the probiotic strain Lactobacillus plantarum Ep‐M17 inhibits the growth of V. parahaemolyticus E1 in vitro.

Selenoprotein is a feed additive that can overcome oxidative stress in intensive Pacific white shrimp (Litopenaeus vannamei) culture. This study evaluated the effects of selenoprotein supplementation at various doses on Pacific white shrimp’s digestibility, growth, and health performance.

The present study investigated the growth performance, feed utilization, intestinal morphology, and microbiota communities of juvenile large yellow croaker (Larimichthys crocea) fed diets containing different proportions of soy protein concentrate (SPC) (0, 15%, 30%, and 45%, namely FM, SPC15, SPC30, and SPC45) as a substitute for fish meal (FM) for 8 weeks.

The current research investigated the effects of pot marigold (Calendula officinalis) powder on growth, biochemical parameters, digestive enzymes, serum and mucus immune responses, antioxidant defense, and resistance of rainbow trout (Oncorhynchus mykiss) against Yersinia ruckeri. Fish (No. 450, 15.06 ± 0.11 g; mean ± SE) were randomly distributed to five groups fed with a diet containing 0 (control group), 0.5%, 1%, 1.5%, and 2% of pot marigold powder (MP) for 60 days.

Efforts have been made to find alternatives to fish meal (FM), as the sustainability of aquaculture depends on it. Insect meal (IM) is a potential candidate to partially replace FM, being more sustainable and economically viable. In this experimental trial, three diets were tested with different yellow mealworm incorporation: a control diet with no IM, a diet with an inclusion of 10% IM (Ins10), and a diet with an incorporation of 20% IM (Ins20).

This study investigated tea polyphenols (TP), α‐lipoic acid (ALA) and their joint use on the antioxidant and lipid metabolic performance of hybrid grouper (♀Epinephelus fuscoguttatus × ♂E. lanceolatu) took food with high‐fat diets. Six high‐lipid diets with isonitrogen (50% of dry matter) and isolipid (17% of dry value) were designed, in which a total content of 1,000 mg/kg additives were added to each group except for the control group (FL).

The effects of dietary supplementation with mannan oligosaccharide (MOS) on growth performance, haematological parameters, abundance of intestinal Vibrio spp., immune response, and tolerance to low salinity stress and Streptococcus iniae challenge were evaluated in pompano (Trachinotus ovatus). Pompano (3.24 ± 0.45 g) were fed six diets including a basal diet as control, and the basal diets supplemented with 0.1%, 0.2%, 0.4%, 0.6%, or 0.8% MOSs for 8 weeks.

Abstract Hybrid grouper (E. lanceolatu × E. fuscoguttatus) is a high economic value cultured species in Southeast Asia. There is a dangerous stage during its body length from 7 cm to 15 cm, and rotten fins and enteritis often occur in this period.

Abstract A 100‐day feeding trial was conducted to investigate the influences of dietary lipid levels on the immunity and mechanism in abalone Haliotis discus hannai Ino. Abalones (initial weight: 10.98 ± 0.05 g) were fed with graded levels of dietary lipid, which were 15.73, 23.41, 31.72, 38.25, 46.35, 55.63, 61.70 and 67.19 g/kg.

Abstract An 8‐week trial was performed to evaluate tributyrin effects on growth, disease resistance, lipid metabolizing, distal intestinal inflammation‐, tight junctions‐ and apoptosis‐related gene expressions caused by high‐level cottonseed protein concentrate (CPC) substitution of fishmeal. Fish were fed one fishmeal (50% fishmeal) and six CPC diets (27.5% fishmeal and 25.2% CPC).

Abstract The present study was conducted to investigate the effects of yeast culture on the growth, hepatic antioxidant capacity, intestinal barrier and microflora of fish fed a high‐cottonseed protein diet.

Abstract A 60‐day feeding trial was carried out to investigate the effects of fish protein hydrolysate (FPH) in high lupin meal (LM) diets on growth, health and immunity of barramundi. Fish were fed a fishmeal‐based control diet (FM) and three test diets to replace 75% fishmeal protein in the control diet by lupin meal alone (LM), lupin meal with FPH (LM+FPH) and fermented lupin meal with FPH supplementation (FLM+FPH).

Aquaculture Nutrition, Volume 27, Issue 6, Page 2587-2599, December 2021.

Aquaculture Nutrition, Volume 27, Issue 6, Page 2195-2204, December 2021.

Aquaculture Nutrition, EarlyView. To investigate the potential alleviative effect of taurine supplementation on growth and oxidant stress in freshwater prawn Macrobrachium nipponense (De Haan) exposed to dietary lead, pelleted feeds were prepared with two concentrations of lead (0 and 10 mg/kg diet) and two levels of taurine (0 and 15 g/kg diet). Healthy prawns were randomly distributed between the four treatment groups, with three tanks per group and 50 prawns per tank.

Aquaculture Nutrition, EarlyView. Abstract

Aquaculture Nutrition, EarlyView. A 56-day feeding try was conducted to evaluate the effects of processed garlic known as black garlic (BG) fed at different inclusion levels (0 (BG0, control), 5 (BG0.5), 10 (BG1), 20 (BG2), 40 (BG4), and 80 (BG8) g/kg feed) on the growth, whole body composition, immune and antioxidant enzyme activities, gut microbiota and resistance to Vibrio parahaemolyticus infections in Litopenaeus vannamei (initial weight = 0.60 ± 0.001 g).

Aquaculture Nutrition, EarlyView. The present study was conducted to evaluate the supplemental effects of fermented noni juice on growth performance, immune response, antioxidant status, intestinal microflora and disease resistance of yellow catfish that fed with high‐fat diet. Triplicate groups of yellow catfish (1.00 ± 0.05 g) were fed two levels of lipid (80.00 and 160.00 g/kg dry matter) and two levels of fermented noni juice (0.00 and 20.00 g/kg dry matter) for 56 days.

Aquaculture Nutrition, EarlyView. In the present study, Indian shrimp, Penaeus indicus, were fed on diets supplemented with basil (Ocimum basilicum) oil (BO) to observe the growth performance, antioxidant and innate immune responses, and the resistance against Vibrio parahaemolyticus infection. The animals (5.0–5.5 g) were fed on diets supplemented with 0.0 (control), 1.0, 2.5 and 5 g BO/kg diet over 90 days. After the feeding trial, animals were intramuscularly injected by V.

Aquaculture Nutrition, EarlyView. The present experiment containing two dietary cadmium (Cd) levels (0 and 5 mg/kg) and two lipoic acid (LA) levels (0 and 600 mg/kg) was carried out to investigate the effects of dietary Cd on the growth, antioxidant capacity, immunity and intestine morphology of Macrobrachium nipponense and the alleviation effects of LA. We found that dietary Cd and LA had no significant effects on the growth of prawns.

Fish were fed diets containing dehydrated ginger powder (GgP) to determine its effects on growth, immune response, antioxidant capacity and resistance to Aeromonas hydrophila infection. 540 Nile tilapia were distributed in 36 250 L aquaria and fed six isonitrogenous and isoenergic diets supplemented with six graded levels of GpP: 0, 2.5, 5, 7.5, 10 and 15 g/kg, during 30 days. After that, growth, hemato‐immunological parameters and antioxidant enzymes activities were determined.

A study was conducted to evaluate the effects of various microbial‐derived additives on the growth performance, non‐specific immunity and disease resistance of Litopenaeus vannamei. Shrimp were fed diets supplemented with 5 g/kg multi‐species probiotic (MSP), 5 g/kg dried lactic acid bacteria fermentate (LF), 5 g/kg dried yeast fermentate (YF), 7.5 g/kg cell wall‐broken yeast extract (BY), 5 g/kg multi‐enzyme preparation (MEP), or 50 g/kg complex probiotics and enzyme (CPE).