Research Publications (Food Safety)

LpxD, acyl-ACP-dependent N-acyltransferase, is the third enzyme of lipid A biosynthesis in Gram-negative bacteria. A recent probe-based screen identified several compounds, including 6359-0284 (compound 1), that inhibit the enzymatic activity of Escherichia coli (E. coli) LpxD. Here, we use these inhibitors to chemically validate LpxD as an attractive antibacterial target.

Author(s): Yunkun Li, Puzhen Xia, Jiayu Wu, Anqi Huang, Guixian Bu, Fengyan Meng, Fanli Kong, Xiaohan Cao, Xingfa Han, Guozhi Yu, Xiaofu Pan, Shiyong Yang, Xianyin Zeng, Xiaogang Du

The simultaneous effects of microwave processing variables affecting the microbial quality and preservation of milk bioactive proteins were evaluated.

Author(s): Weili Liu, Hong Zhao, Zhigang Qiu, Min Jin, Dong Yang, Qunying Xu, Hua Feng, Junwen Li, Zhiqiang Shen

Abstract

Background

E.coli is an important foodborne pathogen. Rapid and robust tracking of the source of E. coli is the key step to control foodborne infections.

Author(s): Youngkee Jung, Yoojung Heo, Jaejoong Lee, Amanda Deering, Euiwon Bae

Author(s): Johanne Ellis-Iversen, Anne Mette Seyfarth, Helle Korsgaard, Valeria Bortolaia, Nanna Munck, Anders Dalsgaard

by Sam Abraham, Mark O’Dea, Shafi Sahibzada, Kylie Hewson, Anthony Pavic, Tania Veltman, Rebecca Abraham, Taha Harris, Darren J. Trott, David Jordan

Background: Mycobacterium tuberculosis nicotinamidase/pyrazinamidase (PZAse) is a metalloenzyme that catalyzes conversion of nicotinamide/pyrazinamide to nicotinic acid/pyrazinoic acid. This study investigated whether a metallochaperone is required for optimal PZAse activity.

Cronobacter infections of infants are commonly regarded as due to the ingestion of contaminated feed. The aim of this study was to determine the occurrence of Cronobacter, total coliforms and Escherichia coli in different brands of natural mineral waters as sold in 20L returnable bottles in Rio de Janeiro, Brazil. The quantification of total coliforms and E. coli was performed by Most Probable Number.

Besides the major nutrients, phosphorus (P) is an essential mineral for both the host animal and the porcine gut microbiota. Different strategies including phytase supplementation and more recently lactic acid (LA) are used to enhance the P availability from cereals in pig diets; however, their impact on the gut microbiota has been rarely related to fecal shedding of opportunistic pathogens.

Author(s): Esin Nagihan Kasap, Üzeyir Doğan, Ferah Çoğun, Ender Yıldırım, İsmail Hakkı Boyacı, Demet Çetin, Zekiye Suludere, Uğur Tamer, Nusret Ertaş

In the chicken slices and canned beef B.bacteriovorax induced a E.coli reduction equal to 4.3 and 2.1 logs, respectively, at 6 hours from contamination.♦ E. coli control; ● E. coli test; ▲ Bb control; ■ Bb test. Summary Bdellovibrio bacteriovorus is a predator micro‐organism towards other Gram‐negative bacteria. We tested B. bacteriovorus to control Escherichia coli growth on chicken slices and canned beef.

Recent emergence of mcr‐1‐positive Escherichia coli (MCRPEC) is causing serious concern around the world. Due to poultry‐origin E. coli holding zoonotic potential, the improved understandings of MCRPEC population structure and antimicrobial resistance are critical to public health purposes. This study provided novel insights into the molecular epidemiology of avian‐origin MCRPEC.

The occurrence of multidrug‐resistant zoonotic bacteria in animals has been increasing worldwide. Working in close contact with livestock increases the risk of carriage of these bacteria. We investigated the occurrence of extended‐spectrum beta‐lactamase (ESBL) and plasmidic AmpC beta‐lactamase producing Enterobacteriaceae (ESBL/pAmpC‐PE) and livestock‐associated methicillin‐resistant Staphylococcus aureus (LA‐MRSA) in Finnish veterinarians (n = 320).

Objectives This study investigates the frequency and characteristics of carbapenemase‐producing Escherichia coli/Klebsiella pneumoniae (CPE/K) and extended‐spectrum cephalosporinase‐producing E. coli/K. pneumoniae (ESCE/K) in healthy humans and livestock in rural Cambodia. Additionally, household practices as risk factors for faecal carriage of ESCE/K are identified.

The bacteriological quality and safety of ready‐to‐eat (RTE) foods in Barbados were assessed in two separate studies conducted between 2014 and 2016. In Study 1, 206 food samples were processed for Salmonella spp. by conventional bacteriological methods. Polymerase chain reaction (PCR) and serotyping were used for confirmation. In Study 2, 120 food samples were processed for the total aerobic plate count (TAPC), coliform count, and E. coli count using conventional bacteriological methods.

Summary

Publication date: Available online 12 August 2019

Source: LWT

Author(s): Mahmut Kilicli, Mehmet Baslar, M. Zeki Durak, Osman Sagdic

Sunflower soapstock inclusion in the diet of fattening lambs modifies meat quality traits and food‐safety.

Abstract

Publication date: September 2019

, Volume 123

Author(s): Myrsini Kakagianni, Konstantinos P. Koutsoumanis

Available online 4 May 2019

Author(s): Aixia Xu, Shihyu Chuang, O. Joseph Scullen, Lihan Huang, Shiowshuh Sheen, Lee-Yan Sheen, James R. Johnson, Christopher H. Sommers

Abstract

Available online 21 March 2019

Author(s): Shan Zhou, Shengyong Zhang, Mingshu Wang, Anchun Cheng, Dekang Zhu, Shun Chen, Mafeng Liu, Xinxin Zhao, Renyong Jia, Qiao Yang, Ying Wu, Shaqiu Zhang, Yunya Liu, Yanling Yu, Ling Zhang, Xiaoyue Chen

Significance and Impact of the Study: PCR screening revealed the significant presence of the genetic markers of Shiga toxin‐producing Escherichia coli (STEC) (stx

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