Research Publications (Food Safety)

Mycotoxins are a class of important pollutants that seriously endanger food security, causing massive food waste and substantial economic losses. Zearalenone (ZEN) has become one of the representative type of mycotoxins owing to its wide range of contamination and high detection rate. With increasing ZEN contamination of food and increased biodegradation and targeting of ZEN, the food production industry has sought biodegradation techniques with enhanced safety and adaptability.

Zearalenone (ZON), zearalanone (ZAN) and their phase I metabolites: α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), α-zearalalanol (α-ZAL) and β-zearalalanol (β-ZAL) are compounds with estrogenic activity that are metabolized and distributed by the circulatory system in animals and can access the food chain through meat products from livestock.

Whole-crop wheat silage (WCWS) is an excellent feed material for ruminants. However, microbial fermentation during silage production consumes valuable nutrients, decreasing the quality of silage.

Nivalenol (NIV) is a trichothecene mycotoxin that is more toxic than deoxynivalenol. It accumulates in grains due to infection with Fusarium species, which are the causative agents of scab or Fusarium head blight. An immunoassay, which is a rapid and easy analytical method, is necessary for monitoring NIV in grains. However, a specific antibody against NIV has not been prepared previously. To establish an immunoassay, we prepared NIV, introduced a linker, and generated antibodies against it.

Fusarium (FER) and Gibberella ear rots (GER) are the two most devastating diseases of maize (Zea mays L.) which reduce yield and affect grain quality worldwide, especially by contamination with mycotoxins. Genetic improvement of host resistance to effectively tackle FER and GER diseases requires the identification of stable quantitative trait loci (QTL) to facilitate the application of genomics-assisted breeding for improving selection efficiency in breeding programs.

Mycotoxins can occur naturally in a variety of agriculture products, including cereals, feeds, and Chinese herbal medicines (TCMs), via pre- and post-harvest contamination and are regulated worldwide. However, risk mitigation by monitoring for multiple mycotoxins remains a challenge using existing methods due to their complex matrices.

In the present study, CotA laccase was cloned from Bacillus licheniformis ZOM-1 and expressed in Escherichia coli. In addition to the oxidative degradation of zearalenone (ZEN) and aflatoxin B1 (AFB1), CotA laccase also has the ability to degrade the Alternaria toxin alternariol (AOH). This is the first report of an Alternaria toxin-degrading enzyme.

Mycotoxins are secondary metabolites produced by various kinds of fungi that can induce disease in humans. The fungal species Penicillium expansum produces patulin (C7H6O4), a polyketide lactone mycotoxin found in fruits. Patulin is classified as noncarcinogen; however, recently, it has been associated with harmful effects on the central nervous system.

Mycotoxin exposure in humans is primarily assessed through its occurrence in external sources, such as food commodities. Herein, we have developed a direct competitive ELISA to facilitate the detection of aflatoxin B1 (AFB1), deoxynivalenol (DON), fumonisin (FUM B1/B2), ochratoxin A (OTA), and zearalenone (ZEA) in human serum.

Grain cleaning is the most effective non-destructive post-harvest mitigation strategy to reduce high levels of mycotoxins on account of the removal of mold-infected grains and grain fractions with high mycotoxin content.

We analysed the dynamics of Fusarium spp. and mycotoxin contamination in Swedish cereals during 2004–2018. More than 1400 cereal samples from field trials were included, collected in a monitoring programme run by the Swedish Board of Agriculture. Five Fusarium mycotoxins were quantified with LC-MS/MS and fungal DNA from four species was quantified using quantitative real-time PCR.

A simple, rapid and sensitive vortex-assisted magnetic solid phase extraction (MSPE) method using core–shell structured magnetic covalent organic frameworks (Fe3O4/COF-TpBD) as adsorbents was established for simultaneous determination of aflatoxins, ochratoxins and enniatins in maize before high-performance chromatography tandem spectrometry detection. Fe3O4/COF-TpBD was facilely fabricated, characterized, and applied for simultaneous extraction of ten mycotoxins from maize.

Pathogens and mycotoxins are serious public health risks for humans and food safety in milk. This study concentrated on detecting Staphylococcus aureus and Ochratoxin A (OTA) in 210 pasteurized milk from ten urban Beijing districts to suggest the co-occurrence of S. aureus with toxin-producing genes and OTA in milk and the possible risk. S. aureus was identified by physiological and biochemical experiments and molecular biology experiments, and enterotoxin genes were identified by PCR.

Ochratoxin A (OTA), one of the best-known mycotoxins, causes problems concerning food safety with potential toxic effects in humans and animals. So, it is crucial to develop simple and sensitive methods for the detection of OTA.

A corn flour certified reference material (KRISS CRM 108-01-011) was developed to ensure accurate and reliable measurements of type B trichothecenes (deoxynivalenol, nivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol), and deoxynivalenol-3-glucoside. The material was freeze-dried, ground, sieved, and well-mixed. The final produced CRM was packaged at 14 g per unit and stored at –70 °C.

Journal of the Science of Food and Agriculture, Accepted Article. Aflatoxins are hepatocarcinogenic and immunosuppressive mycotoxins mainly synthesized by Aspergillus flavus, A. parasiticus, and A. nomius in food systems causing negative health impacts to humans and other organisms.

Mycotoxins are generated by a series of fungal pathogens in postharvest fruit, resulting in serious health threat to consumers and great economic loss to the fruit storage industry. The microbial differences between rotten and healthy fruit during storage and their relationship with mycotoxin production have not been fully studied.

Patulin (PAT) is one of mycotoxins that usually contaminates apple juice, and it is not easily detoxified by cysteine (CYS) at room temperature due to the highly acidic conditions based on the Michael addition reaction. However, it could be effectively degraded by a heating treatment at 120 °C for 30 min in the presence of cysteine.

Traditional dry-cured hams are easily contaminated by toxigenic fungi during the fermentation and ripening stages. The detection and positive rates of ochratoxin A (OTA) are the highest among mycotoxins detected in traditional dry-cured hams, indicating that OTA in hams is a potential safety hazard to human health.

Medicinal plants are important in the South African traditional healthcare system, the growth in the consumption has led to increase in trade through muthi shops and street vendors. Medicinal plants are prone to contamination with fungi and their mycotoxins.

Flour samples spiked with ochratoxin A (OTA) (3,10 and 20 μg kg–1), zearalenone (ZEA) (75, 100, and 200 μg kg–1), and deoxynivalenol (DON) (500, 750, and 1000 μg kg–1), and combined mycotoxins (OTA, ZEA and DON at 20,

Sensitive detection for deoxynivalenol (DON) should be developed due to DON as a kind of harmful mycotoxins which can poses health risk to human health even at low concentrations. In this work, an electrochemiluminescence (ECL) DON aptasensor was proposed based on Ti3C2 dots/Ti3C2 nanosheet (TDTN). Compared with Ti3C2 dots and Ti3C2 nanosheet, the ECL intensity of TDTN was 4 times of Ti3C2 dots and 2 times of Ti3C2 nanosheet as emitters.

Due to the technical challenges of small binding aptamer development, reliable computational simulation studies can be considered as effective tools to design novel and high functional mycotoxin aptameric probes. Here, two novel aflatoxin B1(AFB1) binding aptamers were successfully exploited as recognition elements in the lateral flow aptasensors and the reflective phantom interface (RPI) platform.

Deoxynivalenol is one the of most common mycotoxins in cereals and grains and causes a serious health threat to poultry and farm animals. Our previous study found that DON decreased the production performance of laying hens. It has been reported that DON could exert significant toxic effects on the intestinal barrier and microbiota.

Mycotoxins are toxic secondary fungal metabolites that contaminate feeds, and their levels remain stable during feed processing. The economic impact of mycotoxins on animal production is mainly due to losses related to direct effects on animal health and trade losses related to grain rejection. Deoxynivalenol (DON) is a trichothecene mycotoxin with a worldwide prevalence of about 80%, which induces many toxic effects on human and animal health.