Research Publications (Food Safety)

Mechanism of how anti‐microbial resistance is acquired through food and food residues. Summary Residue and resistance in food animals pose significant challenges to Southeast Asia's public health, animal welfare, agricultural productivity, and sustainability. This article emphasises the need for policy interventions to address these issues effectively. Residue, the presence of harmful substances in animal products, is a concern due to the misuse of veterinary drugs and pesticides.

Tetracyclines (TCs) are among the most widely used antibiotics worldwide for treating bacterial infections (both Gram-positive and Gram-negative) in humans and animals. In Brazil, chicken is one of the most produced and traded meats, with TCs being the primary antibiotics used in its production.

Milk obtained from Yaks, yak hybrid (Dzomo) and hill cattle (Gauri) is major source of nutrients for people inhabiting Himalayas. In present study, QuEChERS approach together with HPLC-UV was used for detection and quantification of tetracycline residues in 170 raw milk samples of hill cattle (60), dzomo (58) and yaks (52) collected from high altitude regions of Himachal Pradesh, a Western Himalayan state of India.

Ultraprocessed foods represent a severe concern to human health due to their direct link with metabolic diseases. Among these foods, mechanically separated meat‐based products are of particular interest due to the use of preservatives and the possible presence of antibiotic residues free or bound to animals’ bone fragments.

This work used Oreochromis sp. residues to obtain collagen. The collagen obtained was blended with chitosan to obtain monolayers and assembled with polylactic acid and polycaprolactone monolayers to obtain bilayers (with promising properties for food packaging). Furthermore, the barrier and mechanical properties of the monolayers can be complemented by assembling bilayers.

Abstract Milk, as a widely consumed nutrient‐rich food, is crucial for bone health, growth, and overall nutrition. The persistent application of veterinary drugs for controlling diseases and heightening milk yield has imparted substantial repercussions on human health and environmental ecosystems. Due to the high demand, fresh consumption, complex composition of milk, and the potential adverse impacts of drug residues, advanced greener analytical methods are necessitated.

The global antibiotic resistance crisis, driven by overuse and misuse of antibiotics, is multifaceted. This study aimed to assess the microbiological and genetic characteristics of raw retail pork meat through various methods, including the isolation, antibiotic susceptibility testing (AST), whole-genome sequencing (WGS) of selected indicator bacteria, antibiotic residue testing, and metagenomic sequencing. Samples were purchased from 10 pre-selected retail stores in Gauteng, South Africa.

Abstract In the evolving field of food safety, rapid and precise detection of antibiotic residues is crucial. This study aimed to tackle this challenge by integrating advanced inkjet printing technology with sophisticated microfluidic paper‐based analytical devices (µPADs). The µPAD design utilized “green” quantum dots synthesized via an eco‐friendly hydrothermal method using green white mulberry leaves as the carbon source, serving as the key fluorescent detection material.

Background Antibiotic residues in milk are a well-known hazard in the dairy food chain. Detection methods for these residues, such as nonspecific microbiological inhibitor tests or group-specific receptor tests, are relatively inexpensive, easy to use, and widely applied to ensure food safety.

Background The presence of veterinary drug residues in food-producing animals and animal products is regulated through the enforcement of maximum residue limits (MRLs). To answer the need of the food sector to monitor these substances in a wide range of food commodities, stakeholders at AOAC INTERNATIONAL identified the need for a reliable confirmatory screening method. Such a qualitative approach is required for compliance checking and to support product release in manufacturing.

Antibiotic residue in food products and the resulting antibiotic-resistant bacteria represent a significant global public health threat. The misuse of antibiotics is a primary contributor to this issue. This study investigated the knowledge, attitudes, and practices (KAP) regarding antibiotic use among cage fish farmers on Ghana’s Volta Lake. Method: We conducted a cross-sectional survey with 91 cage fish farmers across three scales: small, medium, and large.

Abstract Cultured meat, which involves growing meat in a laboratory rather than breeding animals, offers potential benefits in terms of sustainability, health, and animal welfare compared to conventional meat production. However, the cultured meat production process involves several stages, each with potential hazards requiring careful monitoring and control. Microbial contamination risks exist in the initial cell collection from source animals and the surrounding environment.

Tetracyclines (TCs) residues in animal-based foods may cause consumer poisoning or allergic reactions, as well as resistance of pathogenic microorganisms. Therefore, a method to accurately monitor TCs is urgently needed. Here, a sensitive and greenificated molecularly imprinted fluorescent sensor (CDs@MIP) for detecting TCs residues using non-covalent imprinting binding method has been synthesized in a simple and safe manner.

Ivermectin (IVM) is one of the most widely used antiparasitic drugs worldwide and has become the drug of choice for anthelmintic and tick treatment in beef cattle production. Drugs used in production animals requires a withdrawal period after treatment to avoid residual concentrations above the defined maximun residue level (MRL).

A method for the rapid screening and confirmation of 60 food‐borne stimulants in animal‐derived foods was developed using the ultra‐high‐performance liquid chromatography‐quadrupole/orbitrap high‐resolution mass spectrometry (UPLC‐Q/Orbitrap HRMS). After enzymatic hydrolysis at 37°C for 16 h, the samples were extracted with acetonitrile solution. The extraction solution was purified by PRiME HLB pass‐through solid‐phase extraction cartridge and redissolved by nitrogen blowing.

The impact of soil fertilization with animal manure on the spread and persistence of antibiotic resistance in the environment is far from being fully understood. To add knowledge about persistence and correlations between antibiotic residues and antibiotic resistance genes (ARGs) in fertilized soil, a longitudinal soil mesocosm study was conducted.

This study developed and validated an analytical method for the simultaneous identification and quantification of 41 veterinary antibiotic drugs from 9 different classes, including sulfonamides, quinolones, tetracyclines, penicillins, macrolides, nitroimidazoles, cephalosporins, diaminopyrimidines, and amphenicol, in animal-derived food products.

by Daniel Ciccarone, Nicole Holm, Jeff Ondocsin, Allison Schlosser, Jason Fessel, Amanda Cowan, Sarah G. Mars

Background

Illicitly manufactured fentanyls and stimulants are implicated in the escalating US mortality from drug overdose. San Francisco, California (SF) has seen declining fentanyl injection while smoking has increased. Beliefs and behaviors surrounding this development are not well understood.

Methods

Probiotic feed additive use in the swine industry has become increasingly popular as a substitute for antibiotic feed additives. This is due to the overuse of antibiotics in swine diets that has led to antibiotic residues in animal products and bacterial resistance. For this reason, we decided to feed a probiotic to prevent illnesses associated with the transition from the nursery to finisher phase.

Sulfonamide antibiotics with superior performance are widely used in aquaculture. However, the residues of sulfonamide antibiotics in aquatic products may pose a threat to human health. In this work, a novel type of magnetic molecularly imprinted polymer (mMIP) was synthesized and coupled with ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) for the selective separation and determination of 4 sulfonamide antibiotics residues in tilapia.

From a public health point of view, antimicrobial residues pose various problems, including potential risks for consumers. The use of some of these antibiotics may affect the form and quality of animal products or affect the quality or output of food processing. Consequently, there is a pressing need for further investigation into the effects of antibiotics on animal products.

IntroductionControl of Campylobacter from farm to fork is challenging due to the frequent emergence of antimicrobial-resistant isolates. Furthermore, poultry production systems are known reservoirs of Campylobacter. The twin-arginine translocation (Tat) pathway is a crucial bacterial secretion system that allows Campylobacter to colonize the host intestinal tract by using formate as the main source of energy.

An HPLC-MS/MS multi-class method for quantitation of 15 different classes of veterinary drug residues (>140 analytes) in milk and poultry feed was developed and validated. Accuracy criteria for routine laboratories were met for the majority of analytes, > 83 % in milk and between 50 and 60 % in chicken feed, with an apparent recovery of 60–140 %.

Abstract