Research Publications (Food Safety)

This study aimed to determine the prevalence of Staphylococcus aureus subclinical mastitis and to genotype the S. aureus isolates using the 16S-23S rRNA intergenic spacer (RS-PCR) method. In addition, the genes responsible for adherence, biofilm formation, host evasion, tissue necrosis, methicillin resistance, and enterotoxin production of S. aureus were investigated. The overall prevalence of S.

Our study was undertaken to select determine the best samples and selective-differential plating media to be used for Salmonella spp. isolation. We also compared among hematological and serum biochemical values, Salmonella biovar Gallinarum (SG) detection (isolation and serological test), and inflammatory intestinal response (fecal leukocyte) in laying hens with naturally occurring fowl typhoid outbreaks.

Genotyping of Staphylococcus aureus isolated from mastitis has become a fundamental tool to understand its complex epidemiology and to evaluate spillover events. The aim of this study was to describe the frequency of genotypes of the S. aureus strains isolated from intramammary infections by spa typing technique, and to evaluate the association between genotypes and the ability to form biofilm under in vitro conditions.

Toxoplasma gondii is an obligate intracellular parasite belonging to the phylum Apicomplexa, which causes the zoonotic disease toxoplasmosis. T. gondii infects almost all warm blood animals. Generally, infected animals are asymptomatic and remain infected for life. Infection of humans occurs by consumption of infected undercooked meat or contaminated vegetables, fruit and water.

This study aimed to determine the frequency and characterization of Salmonella isolates from food-producing animals and human diarrheal samples in Shiraz, Iran. Overall, 105 Salmonella isolates were obtained from chicken meat (70/100, 70.0%), beef (19/100, 19.0%), lamb (11/100, 11.0%), and human stool (5/295, 1.7%). S. Infantis (40.9%), S. Enteritidis (29.5%), and S.

Toxoplasma gondii is an intracellular protozoan parasite that infects most warm-blooded animals, including humans, and causes toxoplasmosis. In this study, the pathogenicity of RH strain of T. gondii in broiler chicks was investigated and the susceptible tissues of chicks were clarified. Fourteen-day-old broiler chicks were injected intraperitoneally with 1×107, 1×106, and 1×105 tachyzoites of T. gondii, respectively.

The present study aimed to molecularly characterize Giardia duodenalis from stool samples of humans, dogs, and cats. Molecular analyses were performed on 59 samples that tested positive for G. duodenalis on coproparasitological examinations.

To determine the antibiotypes and frequency of toxin genes in methicillin-resistant Staphylococcus pseudintermedius (MRSP), 281 nasal swab samples were collected from dogs and dog guardians in Abakaliki, Southeastern Nigeria. Antimicrobial susceptibility testing was determined by disc diffusion technique while detection of toxin genes was carried out by PCR. Exactly 41 (28.7%) and 6 (4.3%) MRSP were obtained from dogs and dog guardians respectively.

The aim of this study was to identify three parasitic major abortion agents in all cattle aborted fetus samples and N. caninum and T. gondii in sheep and goat aborted fetus sent to Elazig Veterinary Control Institute during two years. Total genomic DNAs were obtained using a commercial kit. Real-time PCR analysis was performed separately for each agent. Conventional PCR was set up for confirmation of positive samples.

This study aimed to identify molecular markers associated with antimicrobial resistance and genotype in isolates of Campylobacter spp. isolated from broiler and swine flocks due to its importance to one-health. C. jejuni (n=27) and C.

Campylobacter (C.) spp. represent one of the most important causes for food-borne bacterial pathogen in humans worldwide. The aim of this study was to investigate metabolic requirements of two Campylobacter strains of different species based on substrate utilisation (in vitro).

Livestock are commonly affected by gastrointestinal protozoan parasites, including Giardia duodenalis. In small ruminants, G. duodenalis infection may decrease carcass weight and dressing percentage. Current study was done to determine the prevalence, assemblage distribution, and zoonotic significance of Giardia infection in sheep and goats in Shiraz, southwestern Iran.

Hepatitis E virus (HEV) infection as a zoonotic disease virus has been thoroughly investigated in pigs around the world, but there have been few studies in pet animals. Although molecula and serological evidence suggests that numerous other animal species may act as HEV hosts in industrialized countries, domestic pigs and wild boars are the main reservoirs of HEV genotypes 3 and 4 for human infections.

Giardia duodenalis is a well-known flagellated parasite and the causative agent of protozoal diarrhea in animals and humans worldwide. Current study was aimed at determination of G. duodenalis prevalence, genetic variation and zoonotic significance in various species of rodents in Shiraz, southwestern Iran.

On January 2020, an outbreak of acute mortality was detected in the capybara (Hydrochoerus hydrochaeris) population kept in the Cordoba Zoo Conservation Center (CZCC) in Spain. Morbidity and mortality rates were 71%, and the fatality rate was 100%. Macroscopic examination revealed multifocal nodular lesions with fibrinonecrotic exudate in lungs and mesenteric lymph nodes.

Stray cat populations can represent a significant threat of the transmission of zoonotic diseases such as salmonellosis.

Toxoplasma gondii (T. gondii) is a facultative heterogeneous parasite that belongs to Apicomplexa and can infect almost all warm-blooded animals, including ruminants, birds and humans. To date, no information is available about the molecular investigation of T. gondii in large ruminants from Pakistan.

Pigs are one of several host species for Toxoplasma gondii, and consumption of infected pork may lead to toxoplasmosis in humans. The aim of this study was to develop an indirect enzyme-linked immunosorbent assay (ELISA) using surface antigen protein 1 (SAG1) of T. gondii for the diagnosis of porcine toxoplasmosis and to perform a serological investigation of pigs in Shandong province, China.

Introduction and purpose: Yersinia enterocolitica belongs to the family of Enterobacteriaceae and is a psychrophilic pathogen that is associated with foodborne infections. It usually causes gastroenteritis, mesenteric lymphadenitis, and septicemia. This study aimed to molecular detection, biotyping, and serotyping of Yersinia enterocolitica isolated from chicken livers in Tabriz.

As a part of risk analysis for consumption of meat from wild animals, the prevalence of Campylobacter spp. in wild deer and boar in Japan was investigated. C. hyointestinalis subsp. hyointestinalis (C. hyointestinalis) was isolated from 2.8% (7/253) of the wild deer and 22.1% (71/321) of the wild boar examined.

Bovine mastitis is a major infectious disease affecting dairy animals resulting in enormous economic losses, prolonged antibiotic treatment, reduced milk yield and death of livestock.

A retrospective antimicrobial resistance study of nontyphoidal Salmonella enterica isolates from India during 1990–2017 was conducted to study the microbial susceptibility to antibiotics.

Toxoplasma gondii, a globally important food borne zoonotic parasite, infects most of the warm-blooded animals as well as people. Veterinarians and para vets are considered at risk of T. gondii exposure.

Toxoplasma gondii is an obligate protozoan parasite that can infect mammals and birds. Cats are the definitive host of T.