Research Publications (Food Safety)

Abstract Aims Effects of H2O2 producing electrochemical‐bandages (e‐bandages) on methicillin‐resistant Staphylococcus aureus colonization and biofilm removal were assessed using a porcine explant biofilm model. Transport of H2O2 produced from the e‐bandage into explant tissue and associated potential toxicity were evaluated. Methods and Results Viable prokaryotic cells from infected explants were quantified after 48 h treatment with e‐bandages in three ex vivo S.

Journal of Applied Microbiology, Accepted Article. Aims The aim of this study was to develop a novel approach using lateral flow recombinase polymerase amplification (RPA-LF) combined with immunomagnetic separation (IMS) for the rapid detection of Staphylococcus aureus in milk. Methods and results Under optimum conditions, the average capture efficiency values (CEs) for S.

Journal of Applied Microbiology, Accepted Article. The molecular ecology of Staphylococcus aureus, Staphylococcus pseudintermedius, and their methicillin-resistant strains in healthy dogs and cats could serve as good models to understand the concept of bacterial zoonosis due to animal companionship. This study aims to provide insights into pooled prevalence, genetic lineages, virulence, and antimicrobial resistance (AMR) among healthy dogs and cats.

Journal of Applied Microbiology, Accepted Article. Aim To evaluate the antimicrobial capability of sclareol and its derivatives against Staphylococcus aureus and its Methicillin-resistant strain (MRSA). Methods and Results A new series of Boron-Heck-coupled sclareol analogs were prepared by structural modifications at C-15 terminal double bond of sclareol using ultrasonication.

Journal of Applied Microbiology, Volume 133, Issue 3, Page 1146-1155, September 2022. Aims Despite the recent prosperity of shrimp cultivation in China, very little is known about how different shrimp farming models influence the dynamics of Vibrio parahaemolyticus populations and the antibiotic resistance of this bacterium. Methods and Results To this end, we conducted continuous surveillance of V. parahaemolyticus on fo

Journal of Applied Microbiology, Accepted Article. Aims Antimicrobial resistance is one of the highest priorities in global public health with Staphylococcus aureus among the most important microorganisms due to its rapidly evolving antimicrobial resistance. Despite all the efforts of antimicrobial stewardship, research and development of new antimicrobials are still imperative.

Journal of Applied Microbiology, Accepted Article. Aims The purpose of the study was to evaluate the occurrence of Yersinia enterocolitica in different types of wastewater and to characterize the isolates by biotyping, serotyping, and antimicrobial susceptibility testing. In addition, cultivation protocols were evaluated. Methods and Results The occurrence of Y.

Journal of Applied Microbiology, Accepted Article. Aims Norovirus remains the most significant virological risk that is transmitted via food and the environment to cause acute gastroenteritis.

Journal of Applied Microbiology, Accepted Article. Aim This study aimed to examine the inactivation efficacy of plasma-activated acetic acid (PAAA) against Salmonella Typhimurium cells and biofilm and elucidate the underlying the chemical inactivation pathway. Methods and Results PAAA was prepared by discharging plasma to 20 ml of 0.2% (v/v) acetic acid (AA) for 20 min (2.2 kHz and 8.4 kVpp).

Journal of Applied Microbiology, Accepted Article. Aims Bacterial response to temperature changes can influence their pathogenicity to plants and humans. Changes in temperature can affect cellular and physiological responses in bacteria that can in turn affect the evolution and prevalence of antibiotic-resistance genes.

Journal of Applied Microbiology, Accepted Article. Aims The aim was to exploit WGS to assess genomic diversity, identify virulence genes and deduce the proportion of Campylobacter colonised broilers that directly contaminate their carcasses. Methods and Results C.

Journal of Applied Microbiology, Accepted Article. Aims To reduce Salmonella contamination of animal feeds, Salmonella contamination in a feed materials storage facility was investigated, and hazard analysis was performed to acquire the basic information necessary to establish a control method for Salmonella.

Journal of Applied Microbiology, Accepted Article. Aim This study was done to determine the effects of temperature, pH, and sodium chloride (NaCl) on antimicrobial activity of magnesium oxide (MgO) nanoparticles (NPs) against Escherichia coli O157:H7. Methods and Results Culture conditions were established by varying the pH (5.0, 7.2, and 9.0), NaCl concentration (0.5, 2.0, 3.5, and 5.0%, w/v), and incubation temperatures (4, 12, 22, and 37°C).

Journal of Applied Microbiology, Accepted Article. Aims To compare the antimicrobial resistance (AMR) genes in a genetically diverse group of Salmonella enterica recovered from foods imported into England between 2014 and 2018. Methods and Results Whole genome sequence was used to detect AMR resistance genes or chromosomal mutations associated with AMR in Salmonella recovered from edible leaves imported from Asia (n =115) as compared t

Journal of Applied Microbiology, Accepted Article. Aims to characterize a collection of Cronobacter sakazakii isolates collected from various origins in Jordan. Methods and Results the isolates were characterized using 16S rRNA sequencing, DNA microarray, multi-locus sequence typing (MLST), O-serotyping, virulence gene identification, and antibiotic susceptibility testing. The identities and phylogenetic relatedness revealed that C.

Journal of Applied Microbiology, Accepted Article. Vibrio spp. are associated with water and seafood-related outbreaks worldwide. They are naturally present in aquatic environments such as seawater, brackish water and freshwater environments.

Journal of Applied Microbiology, Accepted Article. Aims In the study, seven Plant Growth Promoting Rhizobacteria (PGPR) Azotobacter species were screened against three strains of Fusarium verticillioides to test its antifungal activity. Azotobacter strains were tested for the degradation of fumonisin produced by F.

Journal of Applied Microbiology, Accepted Article. Aims Our objective was to determine how injectable antimicrobials affected populations of Salmonella, Escherichia coli, and Enterococcus spp.

Journal of Applied Microbiology, Accepted Article. Aims The aim of the current study was to investigate the effect of plasma-mediated oxidative stress on the post-treatment viability of Listeria monocytogenes at the physiological and molecular levels. Methods and Results 107 CFU/mL L. monocytogenes in 10 mL phosphate-buffered saline (PBS) were treated with atmospheric nonthermal plasma for 0, 30, 60, 90 and 120 s, respectively.

Journal of Applied Microbiology, Accepted Article. Aims Staphylococcus aureus has emerged as a serious threat to food safety owing to biofilm formation. The study aimed to examine antibiofilm mechanism of a novel milk-derived antimicrobial peptide BCp12 against it. Methods and results Anti-biofilm activity of BCp12 was studied by crystal violet staining, MTT assay, motility, SEM and CLSM.

Journal of Applied Microbiology, Accepted Article. Aims Bacillus cereus is often responsible for foodborne diseases and both local and systemic infections in humans. Cases of infection in other mammals are rather rare. In this study, we report a B.

Journal of Applied Microbiology, Accepted Article. Aims This study aims at immobilization of fungal mutants on nanosilica-carriers for designing efficient biosorbents as a significant new technology for decontamination practices and maximizing their heavy metal (HM) sorption proficiency through the experimental design methodology. Materials and Results Endophytic fungal mutant strains, Chaetomium globosum El26 mutant and Alternaria alternata

Aims

Dry surface biofilms (DSB) survive on environmental surfaces throughout hospitals, able to resist cleaning and disinfection interventions. This study aimed to produce a dual species DSB and explore the ability of commercially available wipe products to eliminate pathogens within a dual species DSB and prevent their transfer.

Methods and Results

Journal of Applied Microbiology, Accepted Article. Aims To evaluate the role of starter inoculums to reduce total fermentation time as well as improve safety and quality of Sheedal, a fermented fish-product of India. Methods and Results Lactiplantibacillus plantarum and Staphylococcus piscifermentans were isolated from the Sheedal and used as starter inoculums.

Journal of Applied Microbiology, Accepted Article.  Aim To assess the biofilm-producing capacities of S. aureus strains isolated from hospitalized patients in Israel. Methods and Results A total of 163 S. aureus (80 MRSA and 83 MSSA) screening (nasal swab) and clinical samples (blood and wounds) were characterized.