Research Publications (Food Safety)

P. hydropiper ethanolic extract: Chlorogenic acid, ferulic acid, rutin, myricetin andquercetin. P. hydropiper essential oil: dodecanal, caryophyllene, caryophyllene oxide, decanal, α‐caryophyllene, citronellol, heptadecanal, linalool and phytol. Ethanolic extract and essential oil of P. hydropiper exhibited antimicrobial activity against three bacteria Staphylococcus aureus 6538P, Escherichia coli ATCC 11229 and Salmonella Typhimurium ATCC 14028 using time‐kill kinetics assay.

Co‐microencapsulation of lactobacilli cell free and propolis extract inhibited bacterial growth considerably. Abstract Cell free extracts (CFE) obtained from Lactobacillus plantarum FI 8595 and Lactobacillus reuteri ATCC 55730 alone or in combination with propolis ethanolic or water extracts (1%) were microencapsulated with maltodextrin (25%) before the subsequent spray drying process. They were morphologically characterized by scanning electron microscope.

Botrytis rot is a serious disease of fruit and vegetables around the globe. We tested and found that frequencies above 5 kHz and mechanical vibrations and acoustic frequencies from cold‐storage devices increase the growth rate of botrytis rot, suggesting that acoustic output from storage units could promote the mold during transport, storage, or within grocery stores.

Antimicrobial peptides (AMPs) are powerful microbial inhibitors with high potential in food preservation, despite this, their use as preservatives is very limited and one of the main reasons is the concern about their stability to food processing conditions. This manuscript offers a different view of the application of AMPs in food preservation, addressing the relationship between food processing parameters such as pH, temperature and proteases, and their influence on antimicrobial activity.

PEF treatment of sesame seeds provides significant reduction on aflatoxin producing fungi, Aspergillus paraciticus, as well as decomposition of aflatoxins with reduced mutagenity with preservation of physical properties and reduced acid number and peroxide value. Abstract Seed processing technologies are essential for seed safety and functionality through protection of physicochemical quality, pathogen inactivation, aflatoxin detoxification and alleviation of mutagenicity.

In this study, the rainbow trout was stored for 18 days. Changes in the resistance levels in head, meat and eyes, changes of color and odor in the head and meat, changes of liquid levels in the skin and meat, were measured with Arduino. Study showed that the resistance value on the meat decreased as the storage period increased; and that the color, odor and liquid level measurement sensors could be used in identifying the food quality.

Coronaviruses, enveloped non‐segmented positive‐sense RNA viruses, distinguished in the mid‐60s can infect humans and a variety of birds and mammals.

• Salmonella enterica subsp.

Escherichia coli O157:H7 entered into the Viable but Non Culturable (VBNC) state under salinity stress of 10 and 30% during production of salted silver carp. VBNC E. coli O157:H7 induced by NaCl stress during salted fish production continued to express its pathogenic genes of stx1, stx2, eae and hly. Abstract Many bacteria including Escherichia coli can enter into the viable but non‐culturable (VBNC) state under unfavorable condition. This study investigated the fate of E.

A microfluidic device was applied to enumerate Escherichia coli O157:H7 cells in lettuce and beef samples. E. coli O157:H7 inoculated in lettuce and beef were collected with high efficiency by using filtration or two‐step centrifugation. This method could quantify target microbial cells within 1 hr. Abstract Quality assurance is one of the fundamental ways of preventing infections from foodborne pathogens such as Escherichia coli O157:H7, which produces a deadly toxin.

Vibrio parahaemolyticus is one of the most hazardous pathogens causing seafood‐borne diseases in the southern Fujian coast, China. From June to October 2016, a total of 250 samples were collected from retail markets in the Xiamen, Quanzhou, and Zhangzhou regions. Seventy‐seven V. parahaemolyticus isolates were identified using polymerase chain reaction (PCR). Then, molecular typing was performed using repetitive extragenic palindromic‐PCR (REP‐PCR).

Aspergillus, Penicillium, Fusarium, and Alternaria species which produce toxic metabolites, create a big problem in terms of the production of reliable human food and animal feeds. This study was conducted to determine the mycotoxin contents of foodstuff collected uniformly in each year of 2017, 2018, and 2019 in Şebinkarahisar, Turkey by enzyme‐linked immunosorbent assay.

In this study, the effectiveness of ACP in inactivating Aspergillus flavus inoculated to military rations snack using response surface methodology (RSM) was investigated. Additionally, the effect of this treatment on the total count and yeast–mold count, as well as some quality properties of military rations snack, was examined.

A new selective broth, SSEL, was developed for simultaneous enrichment of S. enterica, S. aureus, E. coli O157: H7, and L. monocytogenes, optimizing by single factor experiments and a L9 (34) orthogonal test.

Isochoric freezing, different from isobaric (conventional) freezing, has a superior capability to reduce foodborne pathogens (a) photo and (b) schematic of a prototypical isochoric chamber. Abstract Isochoric freezing, different from isobaric (conventional) freezing, allows for storage below freezing temperatures without significant damage from ice formation.

We have developed a visual on‐site detection of African swine fever by the denaturation bubble‐mediated strand exchange amplification with colorimetric detection strategy. Combined with rapid extraction, the entire detection process takes only 50 min and only requires a portable metal bath. African swine fever virus was successfully detected in eight different tissues and seven types of pig feed by our method, so our method showed strong anti‐interference ability in complex sample matrix.

Freshly harvested Bambara groundnut seed obtained from rural framers in the Ehlanzeni District Municipality of the Mpumalanga province, South Africa does not have detectable levels of mycotoxins. Freshly harvested Bambara groundnut seed were naturally contaminated with mycotoxin producing isolates from the genera Aspergillus and Fusarium. Abstract Freshly harvested Bambara groundnut (BGN) is occasionally consumed raw and can potentially become infected with mycotoxingenic field fungi.

Antifungal effects of smoldering plants are studied in vitro and pistachio nuts. The smoke of the cinnamon plant had an in vitro fungicidal effect in the PDA medium. No aflatoxin was observed in pistachios treated by cinnamon bark smoke. A reliable method is introduced for pistachio farmers to provide safe products. Abstract Aflatoxin (AF) contamination of the Iranian exporting pistachio has become a major problem in the last decades.

The image of a probe thermometer as a nudge tool significantly improved compliance with and control of the critical control points. Citrus scent with a short inscription significantly improved the hygiene behavior of the food handlers at all critical stages of food preparation. Pictures and short inscriptions turned out to be the most effective nudge tools to improve the food handlers' hygiene behavior.

Two monoclonal antibodies (mAbs) specific to Salmonella were produced from a mouse immunized with a combination of heat‐killed whole‐cell and formalin‐fixed Salmonella enterica serovar Enteritidis (S. Enteritidis) as the antigens. The mAb SE‐13D bound specifically only to S. Enteritidis, while mAb Sal‐06G bound to various members of the Salmonella serogroups B, C, D, E, and I. Neither mAb demonstrated any cross‐reactivity to other bacteria.

This study investigated antimicrobial efficacy of trans‐cinnamaldehyde (TC) and chitosan (CH) along with high pressure processing (HPP) against Cronobacter sakazakii in infant formula. All treatments exhibited varying degrees of C. sakazakii reductions up to undetectable levels. Remarkable deformation and damage in C. sakazakii cells were observed by transmission electron microscopy after the appliation of HPP and bioacive cmpounds.

The developed miniaturized test (s) rapidly detects Escherichia coli/coliforms colorimetrically utilizing specific enzyme substrates and selective agents incorporated in media. High sensitivity and selectivity for target bacteria. A total of 139 milk samples were tested for presence of E.coli/coliforms using developed test (s). Abstract Current investigation was aimed to develop colorimetric tests for rapid detection of Escherichia coli/coliforms. These test (s) for E.

Lateral‐flow immunochromatographic assay (LFIA) was performed on pastry samples incubated for different time‐periods and the degree of coliform contamination evaluated. Pastries contaminated with ~5.0 log10 (cfu/g) coliforms showed LFIA positivity by 3 hr culture, whereas pastries contaminated with 2.0 log10 (cfu/g) coliforms showed LFIA positivity by 9 hr culture.

NaHB4, Te powder, CdCl2 and mercaptopropionic acid under a dark place were synthesized to get CdTe quantum dots (QDs) precursors, the precursors were furtherly reacted with zinc acetate and sulfur acetamide to obtain CdTe/ZnS QDs. The CdTe/ZnS QDs were covalently coupled with streptavidin by EDC•HCL and succinimide in the next step.