AGRICULTURAL BIOSECURITY: NOVEL STRATEGIES TO PREVENT AND DIAGNOSE CLASSICAL SWINE FEVER VIRUS

The overarching goals of this proposal are to develop and establish novel virus-vectored vaccine candidates and diagnostics for CSFV that are DIVA compatible.The Specific Aims are to:Develop virus-vectored vaccine candidates for CSFV using recombinant vesicular stomatitis virus (rVSV) and recombinant orf virus (rORFV) as vector platforms; andEstablish DIVA diagnostics for the CSFV subunit vaccines including indirect and blocking ELISAs.The proposed work includes experimental studies in pigs that will evaluate (i) the immunogenicity; and (ii) the efficacy of the rVSV- and rORFV-vectored vaccine candidates, individually and in combination, for CSFV. Clinical samples from CSFV-vaccinated and -challenged pigs will be used for the establishment of DIVA diagnostic assays.