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<oL> <LI>Optimize agronomic systems and environmental practices, including fertilization and
rotation that minimize inoculum potential of Aspergillus flavus and other mycotoxin-
producing fungi while maximizing corn yield and profits in the Mid-south USA. <LI>Gain
an understanding of the role of crop management practices on the ecology of
Aspergillus flavus and aflatoxin contamination in order to optimize the application
of competitive exclusion products. <LI>Develop economical biologically-based strategies,
including antagonists from soil fungi and bacteria, microbial competitors and
antagonists, and natural compounds from biological sources, to minimize mycotoxins
and their respective fungi in corn, and continue ecological studies on Aspergillus
populations under various management strategies, e.g., rotations, tillage, and cover
crops, and herbicide-resistant crops. <LI>Evaluate insect-resistant and susceptible
maize lines for insect damage and aflatoxin and fumonisin contamination. <li>Evaluate corn accession for aflatoxin resistance. Develop isolines with stable resistance and susceptibility. Cooperate with industry in using molecular markers to develop resistent inbreds/hybrids.
Evaluate potassium fertility as a means to decrease mycotoxins. Evaluate corn
maturity as a management practice to avoid heat and/or drought stress and mycotoxins.
Evaluate double-cropped corn for mycotoxin contamination. Evaluate corn-soybean
rotation to reduce fungal inoculum. Characterize population dynamics of Aspergillus
propagules in soil, air, insects and corn at various ontogeny in BT and conventional
corn. Compare efficacy of non-toxigenic A. flavus strains as biocontrol agents. Optimize surfactant concentration and formulation for improvement of efficacy of non-
toxigenic strains. Determine efficacy of Pichia anomala to control aflatoxin and
fumonisin. Identify and characterize bacteria from corn field soils as biocontrol
agents of Aspergillus and Fusarium. Isolate and identify factors in corn-earn-worm-
resistant corn silks for control of insects and mycotoxins. Cooperate with breeders to develop isolines and inbreds/hybrids.