Analysis of GI Community Shifts in Response to Dietary Fiber

In this one year study we will establish through the use of available sequence data, and experimental manipulations of diets, the major bacterial members of the porcine cecum and colon increasing in response to cellulose in the diet and compare these to bacterial 16S rRNS gene sequences found in the human gut.
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Thorough literature review to evaluate know factors influencing gut microflora, generate community patterns based on 16SrRNA gene T-RFLP analysis and establish key members of the porcine microbial community increasing in abundance in repsonse to fiber in the diet and design DNA microarray for the purpose of efficient analysis of the GO microbial community.