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ASSESSING THE ROLE OF HEMOLYSIN BL IN CYTOTOXICITY AND FITNESS OF B. CEREUS IN FOOD SYSTEMS

Objective

The goal of this project is two-fold: (i) to evaluate the effect of polymorphisms in Bacillus cereus gene encoding for an enterotoxin hemolysin BL on transcription and cytotoxicity, and (ii) to assess how the expression of Hemolysin BL affects isolates' ability to colonize surfaces and biofilms. These goals will be completed using the following two objectives:Evaluate the effects of single nucleotide polymorphisms in the hbl genes and corresponding regulatory sequence PlcR-box on the transcription of Hemolysin BL and cytotoxicity towards human colon cells CaCo-2.Examine the effect of Hemolysin BL on surface colonization and biofilm formation.

Investigators
Chandross-Cohen, T.
Institution
PENNSYLVANIA STATE UNIVERSITY
Start date
2024
End date
2027
Project number
PENW-2023-11510
Accession number
1032558